Prevalence and Genetic Diversity of Bacillus cereus Isolated from Raw Milk and Cattle Farm Environments

Peng, Fei; Yuan, Xiujuan; Zhao, Shengjuan; Yang, Tao; Xiang, Jinle; Chen, Xi; Zhou, Lianxin; Ji, Mengdi

doi:10.1007/s00284-019-01741-5

Cited by 16 publications

(18 citation statements)

References 27 publications

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“…B. cereus isolates were most common in liquid manure samples (100.0%, 8/8), followed by bedding (93.3%, 42/45), feces (78.9%, 15/19), feed (41.2%, 7/17), and raw milk (9.8%, 20/205). Fei et al ( 2019 ) isolated only 2 (4%) B. cereu s strains from 50 raw bovine milk samples and 54 isolates (18%) from 300 environmental samples. In that study, bedding (8 isolates, 40%) and excrement (8 isolates, 40%) had the highest prevalence.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, 47 strains of B. cereus sl were classified into 14 STs. Similarly, Fei et al ( 2019 ) divided 54 B. cereus isolates into 18 STs, Zhuang et al ( 2019 ) clustered 84 isolates into 24 STs, Zhao et al ( 2020 ) divided 54 isolates into 24 STs, and Chang et al ( 2021 ) classified 96 B. cereus isolates into 41 STs, illustrating that B. cereus sl from raw milk and farm environment has a high genetic diversity. We found ST1150 was the main ST of B. cereus sl among samples.…”

Section: Discussionmentioning

confidence: 99%

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Characterization and spoilage potential of Bacillus cereus isolated from farm environment and raw milk

Liu²,

Hu³

et al. 2022

Front. Microbiol.

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Bacillus cereus sensu lato (B. cereus sl) is important spoilage bacteria causing milk structure and flavor changes and is ubiquitous in the environment. This study addresses the biodiversity, toxicity, and proteolytic activity of B. cereus sl from 82 environmental samples and 18 raw bovine milk samples from a dairy farm in the region of Tianjin. In sum, 47 B. cereus sl isolates were characterized through biochemical tests, 16S rRNA gene sequencing, and panC gene analysis. Fourteen sequence types (STs) of B. cereus sl were found in raw bovine milk samples, and five new STs (ST2749, ST2750, ST2751, ST2752, and ST2753) were identified in this study. ST1150 was the dominant ST, associated with fecal, air, drinking water, teat skin, teat cup, and teat dip cup. The results of toxin gene analyses showed that 12.77% and 8.51% of isolates carried hblACD and nheABC operons, respectively. In addition, the detection rate of emetic cesB gene was 21.28%. B. cereus sl demonstrated high spoilage potentials even at 7°C, which has the proteolytic activity of 14.32 ± 1.96 μmol of glycine equivalents per ml. Proteolytic activities were significantly (p < 0.05) decreased after the heat treatment. The residual activity of protease produced at 7°C was significantly higher than that produced at 25°C and 37°C after treatment at 121°C for 10 s and 135°C for 5 s (p < 0.01). Together, the results provide insights into the characteristics of B. cereus sl from farm environment and raw bovine milk and revealed that B. cereus sl contamination should also be monitored in raw milk for ultra-high temperature (UHT) products. This knowledge illustrates that strict cleaning management should be implemented to control B. cereus sl and assure high-quality milk products.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization and spoilage potential of Bacillus cereus isolated from farm environment and raw milk

Liu²,

Hu³

et al. 2022

Front. Microbiol.

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“…For instance, infant formula in the Liaoning province (42%) was contaminated with B. cereus more seriously than that in Yunnan province (12%) ( Table 1 ). The regular rates of B. cereus in other cities or provinces, such as Beijing, Liaoning, Gansu, Yunnan, northeast China, were found to be 30%, 27%, 19%, 10% and 16%, respectively [ 13 , 75 , 76 , 77 ]. Among these data, Yunnan province and the Northeast China indeed had a relatively low prevalence of B. cereus in powdered infant formula (PIF).…”

Section: Prevalence Of Bacillus Cereus From Daimentioning

confidence: 99%

Characterization of Bacillus cereus in Dairy Products in China

Liu

et al. 2020

Toxins

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Bacillus cereus is a common and ubiquitous foodborne pathogen with an increasing prevalence rate in dairy products in China. High and unmet demands for such products, particularly milk, raise the risk of B. cereus associated contamination. The presence of B. cereus and its virulence factors in dairy products may cause food poisoning and other illnesses. Thus, this review first summarizes the epidemiological characteristics and analytical assays of B. cereus from dairy products in China, providing insights into the implementation of intervention strategies. In addition, the recent achievements on the cytotoxicity and mechanisms of B. cereus are also presented to shed light on the therapeutic options for B. cereus associated infections.

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“…Among MLST approaches that rely on multiple housekeeping genes, the PubMLST seven-gene MLST scheme for "B. cereus" (which relies on the glp, gmk, ilv, pta, pur, pyc, and tpi loci) became, and remains, a popular framework for characterizing B. cereus s.l. isolates (Jolley, Bray, and Maiden 2018;Jolley and Maiden 2010;Priest et al 2004), including strains associated with outbreaks, illnesses, and/or infections (Akamatsu et al 2019;Aoyagi et al 2020;Barker, Thakker, and Priest 2005;Carroll et al 2019;Hoffmaster et al 2008;Saikia et al 2019), food and food-associated environments (Cardazzo et al 2008;Fei et al 2019; Thorsen et al 2015;Yang et al 2017;Yu et al 2019;Yu et al 2020;Zhao et al 2020;Zhuang et al 2019), and those with biocontrol properties (Raymond and Federici 2017;Raymond et al 2010). For simplicity, in the sections below, we primarily discuss wellcharacterized B. cereus s.l.…”

Section: Introductionmentioning

confidence: 99%

Keeping up with the Bacillus cereus group: taxonomy through the genomics era and beyond

Carroll¹,

Cheng

Wiedmann

et al. 2021

Critical Reviews in Food Science and Nutrition

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The Bacillus cereus group, also known as B. cereus sensu lato (s.l.), is a species complex that contains numerous closely related lineages, which vary in their ability to cause illness in humans and animals. The classification of B. cereus s.l. isolates into species-level taxonomic units is thus essential for informing public health and food safety efforts. However, taxonomic classification of these organisms is challenging. Numerous-often conflicting-taxonomic changes to the group have been proposed over the past two decades, making it difficult to remain up to date. In this review, we discuss the major nomenclatural changes that have accumulated in the B. cereus s.l. taxonomic space prior to 2020, particularly in the genomic sequencing era, and outline the resulting problems. We discuss several contemporary taxonomic frameworks as applied to B. cereus s.l., including (i) phenotypic, (ii) genomic, and (iii) hybrid nomenclatural frameworks, and we discuss the advantages and disadvantages of each. We offer suggestions as to how readers can avoid B. cereus s.l. taxonomic ambiguities, regardless of the nomenclatural framework(s) they choose to employ. Finally, we discuss future directions and open problems in the B. cereus s.l. taxonomic realm, including those that cannot be solved by genomic approaches alone.

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Prevalence and Genetic Diversity of Bacillus cereus Isolated from Raw Milk and Cattle Farm Environments

Cited by 16 publications

References 27 publications

Characterization and spoilage potential of Bacillus cereus isolated from farm environment and raw milk

Characterization and spoilage potential of Bacillus cereus isolated from farm environment and raw milk

Characterization of Bacillus cereus in Dairy Products in China

Keeping up with the Bacillus cereus group: taxonomy through the genomics era and beyond

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