Prevalence and genotyping of bovine Cryptosporidium species in the Mediterranean and Central Anatolia Region of Turkey

Yıldırım, Alparslan; Adanir, Ramazan; İncı, Abdullah; Yukari, Bayram Ali; Düzlü, Önder; Önder, Zühal; Çiloğlu, Arif; Şimşek, Emrah

doi:10.1016/j.cimid.2020.101425

Cited by 28 publications

(28 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Of the 11 subtypes detected (IIaA11G2R1, IIaA11G3R1, IIaA12G3R1, IIaA13G2R1, IIaA13G4R1, IIaA14G1R1, IIaA14G3R1, IIaA15G2R1, IIdA16G1, IIdA18G1, IIdA22G1) in 82 cases (70 calves, 10 lambs and 2 goat kids), only one (IIaA15G2R1, identified in 4 calves and 3 lambs) was found in our study. Another recent study from Turkey identified C. parvum in 138 of 550 calves and heifers (73). Gp60 gene sequence analysis reveled only two subtypes (IIaA13G2R1, IIaA14G1R1) in all 138 samples, none of which were identified in our study.…”

Section: Discussioncontrasting

confidence: 44%

Dairy Calves in Uruguay Are Reservoirs of Zoonotic Subtypes of Cryptosporidium parvum and Pose a Potential Risk of Surface Water Contamination

et al. 2020

View full text Add to dashboard Cite

show abstract

Section: Discussioncontrasting

confidence: 44%

Dairy Calves in Uruguay Are Reservoirs of Zoonotic Subtypes of Cryptosporidium parvum and Pose a Potential Risk of Surface Water Contamination

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Moreover, IIaA13G2R1 was found the most common subtype in pre-weaned diarrheic calves in the Central Anatolia region of Turkey [21]. This uncommon zoonotic subtype was also reported in Algerian young calves, lambs, and goat kids [41,42].…”

Section: Discussionmentioning

confidence: 91%

“…Except for one calf which was infected with C. bovis, all of the Cryptosporidium from 90 calves, 13 lambs and 2 goat kids were of C. parvum. In Turkey, there is only one study reporting C. bovis in calves in the Mediterranean and Central Anatolia regions [21]. In an another report C. bovis was detected in environmental water in Turkey [35].…”

Section: Discussionmentioning

confidence: 99%

“…Since then, Cryptosporidium oocysts have been reported in animals with and without diarrhea from Kars [18], Erzurum [19], and Van provinces of Turkey [20]. Recently, a study mentioned C. parvum was the predominant species in pre-weaned calves whereas C. bovis and C. ryanae were mostly found in postweaned calves and heifers in the Mediterranean and Central Anatolia regions of Turkey [21]. Another study reported the distribution of C. parvum in free-range pre-weaned diarrheic calves and goat kids in five provinces of Turkey [22].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular detection of genotypes and subtypes of Cryptosporidium infection in diarrheic calves, lambs, and goat kids from Turkey

Kabir

Ceylan

et al. 2020

Parasitology International

View full text Add to dashboard Cite

Background: Cryptosporidium spp. are enteric protozoan parasites that infect a wide range of animals as well as humans. The studies on Cryptosporidium infections of animals in Turkey are mostly rely on microscopic observation. Few data are available regarding the distribution of Cryptosporidium genotypes and subtypes infection. The aim of this study is to analyse the zoonotic potential of Cryptosporidium oocysts shed from young ruminant livestock. Methods: A total of 415 diarrheic fecal specimens from 333 calves, 67 lambs, and 15 goat kids were examined for the presence of Cryptosporidium oocysts by microscopy. Microscopic positive specimens were then analyzed for Cryptosporidium genotypes and subtypes detection by use of nested PCR of the small subunit ribosomal RNA (SSU rRNA) gene and the highly polymorphic 60 kDa glycoprotein (gp60) gene followed by sequence analyses. Results: The results of this study revealed that 25.6% (106 of 415) of the specimens were positive for Cryptosporidium spp. infection by microscopic examination and molecular analysis. We identified 27.4% (91/333), 19.4% (13/67), and 13.4% (2/15) of positivity in calves, lambs and goat kids, respectively. Genotyping of the SSU rRNA indicated that almost all positive specimens were of C. parvum , except for one calf which was of C. bovis. Sequence analysis of the gp60 gene revealed the most common zoonotic subtypes (IIa and IId) of C. parvum. We detected 11 subtypes (IIaA11G2R1,

show abstract

“…(61.5%). Yildirim et al [ 50 ] investigated the Cryptosporidium species distribution in Turkey which also included the same region that the calves originated from in this study. Results showed that 70.8% isolates were C. parvum .…”

Section: Discussionmentioning

confidence: 99%

Seasonal and Age-Associated Pathogen Distribution in Newborn Calves with Diarrhea Admitted to ICU

Berber

Çanakoğlu

Sözdutmaz

et al. 2021

Veterinary Sciences

Self Cite

View full text Add to dashboard Cite

Calf mortality constitutes a substantial loss for agriculture economy-based countries and is also a significant herd problem in developed countries. However, the occurrence and frequency of responsible gastro-intestinal (GI) pathogens in severe newborn diarrhea is still not well known. We aimed to determine the seasonal and age-associated pathogen distribution of severe diarrhea in newborn calves admitted to the intensive care unit (ICU) of Erciyes University animal hospital over a year. Fecal samples were collected during the ICU admissions, and specimens were subjected to a diarrheal pathogen screening panel that included bovine coronavirus (BCoV), Cryptosporidium spp., ETEC K99+, and bovine rotavirus, using RT-PCR and conventional PCR methods. Further isolation experiments were performed with permissive cell cultures and bacterial enrichment methods to identify the clinical importance of infectious pathogen shedding in the ICU. Among the hospitalized calves aged less than 45 days old, the majority of calves originated from small farms (85.9%). The pathogen that most frequently occurred was Cryptosporidium spp. (61.5%) followed by rotavirus (56.4%). The frequency of animal admission to ICU and GI pathogen identification was higher during the winter season (44.9%) when compared to other seasons. Most calves included in the study were 1–6 days old (44.9%). Lastly, co-infection with rotavirus and Cryptosporidium spp. occurred more frequently than other dual or multi-infection events. This study was the first to define severe diarrhea—causing GI pathogens from ICU admitted newborn calves in Turkey.

show abstract

Prevalence and genotyping of bovine Cryptosporidium species in the Mediterranean and Central Anatolia Region of Turkey

Cited by 28 publications

References 70 publications

Dairy Calves in Uruguay Are Reservoirs of Zoonotic Subtypes of Cryptosporidium parvum and Pose a Potential Risk of Surface Water Contamination

Dairy Calves in Uruguay Are Reservoirs of Zoonotic Subtypes of Cryptosporidium parvum and Pose a Potential Risk of Surface Water Contamination

Molecular detection of genotypes and subtypes of Cryptosporidium infection in diarrheic calves, lambs, and goat kids from Turkey

Seasonal and Age-Associated Pathogen Distribution in Newborn Calves with Diarrhea Admitted to ICU

Contact Info

Product

Resources

About