Prevalence and genotyping of Chlamydia trachomatis in symptomatic male patients from Istanbul, Turkey

Köksal, Muammer Osman; Beka, Hayati; Demırci, Mehmet; Kadıoğlu, Ateş; Ağaçfidan, Ali; Akgül, Baki

doi:10.1186/s40064-016-3370-3

Cited by 5 publications

(5 citation statements)

References 18 publications

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“…15,39,40 In many countries, these three genotypes have also been reported more commonly in urogenital infections. 38,41 However, our findings were slightly different from reports from some countries. Genotypes D, F, and K are the most prevalent among STD subjects in Thailand, while genotypes F predominate in Mexico and Brazil.…”

Section: Discussioncontrasting

confidence: 99%

Prevalence, risk factors and genotyping of chlamydia trachomatis from endocervical specimens of infertile women at a tertiary care hospital, Vietnam

Nguyen,

Do Ngoc,

Nguyen Le

et al. 2024

Int J STD AIDS

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Background To our knowledge, the prevalence, risk factors and distribution of C. trachomatis genotypes are rarely mentioned in Vietnam. This study aimed to find the prevalence, risk factors and distribution of C. trachomatis genotypes in infertile Vietnamese women. Methods Endocervical swabs were collected from infertile women at the National Hospital of Obstetrics and Gynecology, Vietnam, between January 2020 and December 2021. All samples were analyzed for C. trachomatis presence by Cobas 4800 CT/NG Test. Sequencing methods of ompA gene were used to determine the C. trachomatis genotypes. An approximately 1200 bp ompA fragment was aligned with reference sequences from GenBank to identify the corresponding genotype. Results The prevalence of endocervical C. trachomatis infection was 15.6% of 761 participants. Factors independently associated with CT infection among infertile women, obtained by multivariate analysis, included abnormal vaginal discharge, cervicitis, lower abdominal pain, a history of ectopic pregnancy, having more than one sex partner, and age at first intercourse. Among the samples, genotype E (25.93%) was most frequently found, followed by genotypes D/Da (22.23%), F (13.58%), G/Ga (12.35%), J (12.35%), H (6.17%), K (3.70%), B/Ba (2.47%), and I/Ia (1.23%), respectively. Genotype F was related to types of infertility, and genotype H was associated with a history of miscarriage. Conclusions The present study indicated a high prevalence of C. trachomatis in infertile Vietnamese women. The most common genotypes found in this population were E, D, and F. Our findings suggest that routine screening is necessary for early detection and performance of infection control methods.

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Section: Discussioncontrasting

confidence: 99%

Prevalence, risk factors and genotyping of chlamydia trachomatis from endocervical specimens of infertile women at a tertiary care hospital, Vietnam

Nguyen,

Do Ngoc,

Nguyen Le

et al. 2024

Int J STD AIDS

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“…In the study of Köksal et al, conducted in Turkey, genotype E was the most common type, but the prevalence of type F was much lower than that of other types, corresponding to only 1.8% of cases. In this study, types G and H were common after type E in terms of prevalence [ 26 ]. These results showed that the distribution of genotypes in different geographical areas could be different, but the important point is that type E in all studies is one of the most common genotypes of this bacterium, and globally, it seems to be more common than other genotypes of bacteria.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Chlamydia trachomatis Genotypes in Endocervical Specimens by Sequence Analysis of ompA Gene among Women in Tehran

Rajabpour,

Emamie,

Pourmand

2023

Journal of Tropical Medicine

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Tehran’s actual prevalence of Chlamydia trachomatis (CT) and its genotypes are still unclear. Molecular typing of CT strains can provide essential epidemiological knowledge and contribute to improved control measures. In this study, we aimed to determine the prevalence of CT and its genotypes in the endocervical infections of females who attended the gynecology and infertility clinics in Tehran. A total of 291 women were tested for chlamydial infection by in-house PCR using specific primers for the CT cryptic plasmid. Nested PCR for amplification of the ompA gene in positive samples was carried out, genotyping was performed by sequencing this gene, and further phylogenetic analysis was conducted. Sexual infection by CT was observed in 10.3% (30/291) of the subjects, and the mean age of patients was 30.4. The ompA gene was sequenced in 27 samples, revealing E genotypes 40.7%, (n = 11), F 25.9%, (n = 7), G 18.5%, (n = 5), D 11.1%, (n = 3), and K 3.7%, (n = 1). This study emphasizes the importance of the diversity among CT genotypes in our studied population and the need for wide-screening the neglected bacterial infection among women in Tehran.

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“…Briefly, the microorganism genotyping is based on ompA sequencing, which is amplified by using the primers that have been described by Jalal and colleagues [ 73 ] and following the protocol that has been detailed by Bianchi et al [ 67 ], in which the amplicons must be purified by using, for example, NucleoSpin ® Extract II (Macherey-Nagel GmbH, Germany) or the Qiaquick PCR Purification Kit (Qiagen, Hilden, Germany). Finally, the automated DNA sequencing can occur on the ABI PRISM 3100 genetic analyzer (Applied Biosystem, CA, USA) [ 67 , 74 ]. Next, a bioinformatic analysis must be performed in order to align the sequences of these samples with the sequences within the databases (NCBI GenBank) using bioinformatic tools, such as ClustalX 2.1 multiple aligner, in order to classify the Chlamydia trachomatis strain [ 55 , 67 , 75 ].…”

Section: Chlamydia Diagnostic Methodsmentioning

confidence: 99%

Chlamydia trachomatis as a Current Health Problem: Challenges and Opportunities

Rodrigues

Sousa²,

Vale

2022

Diagnostics

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Chlamydia is one of the most common sexually transmitted bacterial infections (STIs) worldwide. It is caused by Chlamydia trachomatis (CT), which is an obligate intracellular bacterium. In some cases, it can occur in coinfection with other parasites, increasing the pathologic potential of the infection. The treatment is based on antibiotic prescription; notwithstanding, the infection is mostly asymptomatic, which increases the risk of transmission. Therefore, some countries have implemented Chlamydia Screening Programs in order to detect undiagnosed infections. However, in Portugal, there is no CT screening plan within the National Health Service. There is no awareness in the general healthcare about the true magnitude of this issue because most of the methods used are not Nucleic Acid Amplification Technology-based and, therefore, lack sensitivity, resulting in underreporting infection cases. CT infections are also associated with possible long-term severe injuries. In detail, persistent infection triggers an inflammatory milieu and can be related to severe sequels, such as infertility. This infection could also trigger gynecologic tumors in women, evidencing the urgent need for cost-effective screening programs worldwide in order to detect and treat these individuals adequately. In this review, we have focused on the success of an implemented screening program that has been reported in the literature, the efforts made concerning the vaccine discovery, and what is known regarding CT infection. This review supports the need for further fundamental studies in this area in order to eradicate this infection and we also suggest the implementation of a Chlamydia Screening Program in Portugal.

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Prevalence and genotyping of Chlamydia trachomatis in symptomatic male patients from Istanbul, Turkey

Cited by 5 publications

References 18 publications

Prevalence, risk factors and genotyping of chlamydia trachomatis from endocervical specimens of infertile women at a tertiary care hospital, Vietnam

Prevalence, risk factors and genotyping of chlamydia trachomatis from endocervical specimens of infertile women at a tertiary care hospital, Vietnam

Evaluation of Chlamydia trachomatis Genotypes in Endocervical Specimens by Sequence Analysis of ompA Gene among Women in Tehran

Chlamydia trachomatis as a Current Health Problem: Challenges and Opportunities

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