Prevalence and Identity of Sarcocystis Infections in Armadillos (Dasypus novemcinctus)

Lindsay, David S.; McKown, Richard D.; Upton, Steve J.; McAllister, Chris T.; Toivio-Kinnucan, M.; Veatch, Johna K.; Blagburn, Byron L.

doi:10.2307/3284100

Cited by 10 publications

(6 citation statements)

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“…It is probable that the mild lymphocytic inflammation observed in the tongue and the myocardium of nine and 11 animals, respectively, was caused by rupture of protozoan cysts, although cysts were only noted in the tongue of an adult female and in the heart muscle of an adult male. Several Sarcocystis species have been identified in the skeletal muscle and tongue of North and South American armadillos (Howells et al, 1975;Lindsay et al, 1996;Tanhauser et al, 2001), and the prevalence can be as high as 100% (Lindsay et al, 1996). The nine-banded armadillo has been identified as a naturally infected intermediate host of Sarcocystis neurona Tanhauser et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…There have been unconfirmed reports of disease outbreaks of unknown etiology severely affecting wild populations in some areas of Mendoza, but the potential role of diseases in these population reductions remains undefined. Although wild specimens of other armadillo species have been screened for specific pathogens and parasites, such as Leptospira (Carillo et al, 1972), Salmonella (Quevedo et al, 1978), or Sarcocystis (Lindsay et al, 1996), only one study (Mazza et al, 1935) included pichis. The purpose of this study was to describe the lesions affecting wild and captive pichis of Mendoza Province, Argentina.…”

Section: Introductionmentioning

confidence: 99%

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Health Evaluation of Free-Ranging and Captive Pichis (Zaedyus Pichiy; Mammalia, Dasypodidae), in Mendoza Province, Argentina

Superina

Garner²,

Aguilar

2009

Journal of Wildlife Diseases

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ABSTRACT:The health of free-ranging and captive pichis (Zaedyus pichiy) was assessed in Mendoza Province, Argentina, between November 2001 and December 2006. Postmortem examinations of 150 confiscated and vehicle-killed pichis and clinical examinations of 139 wild-caught individuals suggest that the wild populations are currently in good health. Lesions and scars were observed in a large proportion of wild-caught pichis. The most common lesions were associated with parasitism or parasite larva migration. Sarcocystis cysts were relatively common in the skeletal muscle, and Besnoitia cysts were observed in the lungs of 24 evaluated animals. Elevated ambient humidity levels often caused moist dermatitis with epidermal detachment in captive pichis. This report constitutes the first health evaluation of free-ranging and captive Z. pichiy. It will be a starting point for future health studies and will be beneficial for the captive management of this species.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Health Evaluation of Free-Ranging and Captive Pichis (Zaedyus Pichiy; Mammalia, Dasypodidae), in Mendoza Province, Argentina

Superina

Garner²,

Aguilar

2009

Journal of Wildlife Diseases

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“…Howells et al (1975) named S. dasypi and S. diminuta for the armadillo from Brazil. Lindsay et al (1996) reported that S. dasypi , S. diminuta -like sarcocysts, and another undetermined species can infect armadillos throughout the USA. These observations were made before the recognition of armadillos as natural and experimental hosts of S. neurona (Cheadle et al, 2001; Tanhauser et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…Because there are no archived specimens of S. dasypi, the true identity of S. dasypi may never be known. Limited TEM studies indicate that S. dasypi and S. neurona are structurally similar (Lindsay et al, 1996; Dubey et al, 2001b). …”

Section: Discussionmentioning

confidence: 99%

Detection of Sarcocystis spp. infection in bobcats ( Lynx rufus )

Verma

Calero‐Bernal

Lovallo³

et al. 2015

Veterinary Parasitology

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The protozoan Sarcocystis neurona is an important cause of severe clinical disease of horses (called equine protozoal myeloencephalitis, EPM), marine mammals, companion animals, and several species of wildlife animals in the Americas. The Virginia opossum (Didelphis virginiana) is its definitive host in the USA and other animals act as intermediate or aberrant hosts. Samples of tongue and heart from 35 bobcats hunted for fur and food from Mississippi State, USA in February, 2014 were used for the present study. Muscles were examined for Sarcocystis infection by microscopic examination of either unfixed muscle squash preparations or pepsin digests, by histopathology of fixed samples, and by molecular methods. Sarcocystis-like bradyzoites were found in digests of 14 hearts and 10 tongues of 35 bobcats. In histological sections, sarcocysts were found in 26 of 35 bobcats; all appeared relatively thin-walled similar to S. felis sarcocysts under light microscope at 1000x magnification. S. neurona-like sarcocysts having thickened villar tips were seen in unstained muscle squash of tongue of two bobcats and PCR-DNA sequencing identified them definitively as S. neurona-like parasite. DNA extracted from bradyzoites obtained from tongue and heart muscle digests was analyzed by PCR-DNA sequencing at the ITS1 locus. Results indicated the presence of S. neurona-like parasite in 26 of 35 samples. ITS1 sequences identical to S. dayspi were identified in 3 bobcats, 2 of which were also co-infected with S. neurona-like parasite. The high prevalence of sarcocysts in bobcat tissues suggested an efficient sylvatic cycle of Sarcocystis spp. in the remote regions of Mississippi State with the bobcat as a relevant intermediate host.

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“…are ubiquitous parasites and have an obligatory two-host life cycle, with sexual reproduction and sporocyst formation occurring in the definitive host and schizogony and sarcocyst formation occurring in the intermediate host (Gardiner et al 1988). S. dasypi and S. diminuta sarcocysts have been detected from the tongues of armadillos in North America (Lindsay et al 1996). Horses are exposed to multiple Sarcocystis spp.…”

Section: Introductionmentioning

confidence: 99%

Purification of Sarcocystis neurona sporocysts from opossum (Didelphis virginiana) using potassium bromide discontinuous density gradient centrifugation

et al. 2003

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This report describes a new, inexpensive procedure for the rapid and efficient purification of Sarcocystis neurona sporocysts from opossum small intestine. S. neurona sporocysts were purified using a discontinuous potassium bromide density gradient. The procedure provides a source of sporocyst wall and sporozoites required for reliable biochemical characterization and for immunological studies directed at characterizing antigens responsible for immunological responses by the host. The examined isolates were identified as S. neurona using random amplified polymorphic DNA primers and restriction endonuclease digestion assays. This method allows the collection of large numbers of highly purified S. neurona sporocysts without loss of sporocyst viability as indicated by propidium iodide permeability and cell culture infectivity assays. In addition, this technique might also be used for sporocyst purification of other Sarcocystis spp.

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Prevalence and Identity of Sarcocystis Infections in Armadillos (Dasypus novemcinctus)

Cited by 10 publications

References 0 publications

Health Evaluation of Free-Ranging and Captive Pichis (Zaedyus Pichiy; Mammalia, Dasypodidae), in Mendoza Province, Argentina

Health Evaluation of Free-Ranging and Captive Pichis (Zaedyus Pichiy; Mammalia, Dasypodidae), in Mendoza Province, Argentina

Detection of Sarcocystis spp. infection in bobcats ( Lynx rufus )

Purification of Sarcocystis neurona sporocysts from opossum (Didelphis virginiana) using potassium bromide discontinuous density gradient centrifugation

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