Prevalence and Intensity of Thelazia spp. (Nematoda: Thelazioidea) in a Musca autumnalis (Diptera: Muscidae) Population from Central Alberta

O’Hara, James E.; Kennedy, Murray J.

doi:10.2307/3283068

Cited by 15 publications

(13 citation statements)

References 4 publications

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“…The presence of Habronema DNA only in female flies agrees with results of other epidemiological surveys carried out on some species of Thelazia , in which all dissected male flies were shown to be either negative for nematodes or nematode prevalence was very low (O’Hara & Kennedy, 1989; Otranto et al , 2003). However, in the case of habronemosis, the peculiar life‐cycle of stomachworms may imply that both sexes have the same potential to harbour Habronema and infect animals.…”

Section: Discussionsupporting

confidence: 88%

“…The presence of positive samples, from either field‐collected or laboratory‐bred flies, throughout the collection period (June–September) demonstrates that H. microstoma and H. muscae are transmitted during the period of greatest fly activity. Although more positive samples derived from late summer specimens (Table 2), it seems that there is no seasonal fluctuation in the temporal pattern of parasitism by Habronema in fly populations, as has also been demonstrated for bovine Thelazia skrjabini transmitted by the face fly M. autumnalis (O’Hara & Kennedy, 1989).…”

Section: Discussionsupporting

confidence: 52%

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Identification of the intermediate hosts of Habronema microstoma and Habronema muscae under field conditions

Traversa

Otranto

Iorio

et al. 2008

Medical Vet Entomology

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A polymerase chain reaction (PCR)-based assay was used for the specific detection of Habronema microstoma and Habronema muscae (Nematoda, Spirurida) in order to identify the intermediate hosts of both nematode species under field conditions. A total of 1087 netted and 165 laboratory-bred flies were tested. Flies were identified as Musca domestica Linnaeus 1758, Musca autumnalis De Geer 1776, Haematobia irritans (Linnaeus 1758), Haematobia titillans (De Geer 1907) and Stomoxys calcitrans (Linnaeus 1758) (Muscidae). Genomic DNA was extracted from pools of fly heads, thoraces and abdomens, and 703 samples were subjected to a duplex two-step semi-nested PCR assay to specifically detect diagnostic regions within the ribosomal ITS2 sequence of both H. microstoma and H. muscae. Stomoxys calcitrans specimens were positive for H. microstoma DNA and M. domestica specimens were positive for H. muscae DNA. In particular, PCR-positive samples derived from both farm-netted and laboratory-bred flies. The present study represents the first evidence of the vectorial competence of different fly species as intermediate hosts of Habronema stomachworms under field conditions. We discuss the roles of S. calcitrans and M. domestica in transmitting H. microstoma and H. muscae.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 52%

Identification of the intermediate hosts of Habronema microstoma and Habronema muscae under field conditions

Traversa

Otranto

Iorio

et al. 2008

Medical Vet Entomology

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“…Surveys of cattle and horses in North America and England demonstrate that Thelazia spp. are widespread and abundant (5,6,35,36,42,54,62,69,73,80,93,95).…”

Section: Bovine and Equine Thelaziasismentioning

confidence: 99%

BIONOMICS OF THE FACE FLY, MUSCA AUTUMNALIS

Krafsur

Moon

1997

Annu. Rev. Entomol.

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The face fly was introduced from the Palearctic region and spread across North America in 20 years after World War II. Adults feed on cattle and horses, and larvae develop in fresh cattle dung. Little genetic differentiation appears between European and North American populations and among regions within North America. After an autumnally initiated diapause, overwintered flies emerge in spring and reproduce through late spring and summer. Generations after the first overlap, and age structure develops toward a stable age distribution. After three to ten generations, depending on weather, facultative diapause interrupts host feeding and oogenesis, and flies with hypertrophied fat body enter overwintering hibernaculae. Life table statistics and factors affecting population growth and diapause are reviewed. Early views on the fly's effects on animal productivity may have been exaggerated. On-farm control by conventional means has not been effective because of the fly's population dynamics and mobility. We suggest that the alternatives of classical biological control and area-wide control with the sterile insect technique should be considered.

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“…Houseflies in Groups 1 and 2 were examined both by dissection and visual inspection, and using the molecular method as described below. Dissection was carried out by opening the abdomen, as previously described (O'Hara & Kennedy, 1989, 1991), to investigate the presence/absence of eyeworms.…”

Section: Methodsmentioning

confidence: 99%

Musca domestica is not a vector of Thelazia callipaeda in experimental or natural conditions

Otranto

Lia

Testini

et al. 2005

Medical Vet Entomology

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Thelazia callipaeda Railliet and Henry (Spirurida: Thelaziidae), commonly called oriental eyeworm for its widespread presence in the Far East, has been recently found to affect dogs, cats and foxes in northern and southern Italy. Although the biology of T. callipaeda in the definitive hosts has been recently investigated, many doubts still remain about its biology in insect vectors. It has been suggested that more than one species of Diptera, namely Musca domestica Linnaeus (Diptera: Muscidae) and Amiota okadai Maca (Diptera: Drosophilidae), may be involved in the transmission of T. callipaeda in China. The aim of the work described here was to verify the role of M. domestica as a vector of T. callipaeda both in experimental and natural conditions. A total of 310 m. domestica (Group 1) were put in a cage and allowed to feed for 14 days around the eyes of a dog naturally infected by T. callipaeda. Ten flies were collected daily for 14 days. A total of 149 houseflies (Group 2) were fed with T. callipaeda first stage larvae (L1) and dissected at 1, 2 and 7 days post-infection. From June to August 2003, flies were netted (Group 3) in two different sites every 10 days both from the environment and directly from the periocular region of dogs affected by thelaziosis. Musca flies were examined for eyeworms by dissection and visual inspection of house flies (Groups 1 and 2) and using a molecular approach (Groups 1-3) via a specific amplification of the ribosomal internal transcribed spacer 1 (ITS1) sequence of T. callipaeda. On the whole, 180 pools of M. domestica flies were processed molecularly and all the experimentally infected flies (Groups 1 and 2) were found to be negative both at the visual dissection and at the molecular assay. Similarly, the 234 M. domestica collected from Group 3 were negative for T. callipaeda. The results clearly suggest that M. domestica is unlikely to act as a vector of T. callipaeda in southern Europe, in contrast with a single previous report.

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Prevalence and Intensity of Thelazia spp. (Nematoda: Thelazioidea) in a Musca autumnalis (Diptera: Muscidae) Population from Central Alberta

Cited by 15 publications

References 4 publications

Identification of the intermediate hosts of Habronema microstoma and Habronema muscae under field conditions

Identification of the intermediate hosts of Habronema microstoma and Habronema muscae under field conditions

BIONOMICS OF THE FACE FLY, MUSCA AUTUMNALIS

Musca domestica is not a vector of Thelazia callipaeda in experimental or natural conditions

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