Prevalence and metronidazole resistance of Trichomonas vaginalis among Japanese women in 2021

Saito‐Nakano, Yumiko; Umeki, Yuko; Shimokawa, Chikako; Kobayashi, Koichi; Hashimoto, Koichi; Takada, Toshio; Makii, Chinami; Hasebe, Rie; Yoshida, Yasuhiko; Nakajima, Riko; Kobayashi, Seiki; Hisaeda, Hajime

doi:10.1016/j.ijregi.2023.02.007

Cited by 3 publications

(1 citation statement)

References 29 publications

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“…However, persistent and recurrent infections have become a great concern due to increasing drug resistance [ 8 ]. MTZ-resistant isolates have been reported worldwide, with the prevalence ranging from 2.2% to 9.6% across different regions [ 9 – 15 ]. The emergence of TDZ resistance has also been observed, with a reported frequency of 2.0% among the general population of South Africa [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

A fluorescence-based assay for Trichomonas vaginalis drug screening

Chen,

Li,

Wang

et al. 2023

Parasites Vectors

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Background The emergence and spread of drug resistance in Trichomonas vaginalis parasites has become an important concern in trichomoniasis treatment. Fast and reliable growth assessment is critical for validating in vitro drug susceptibility and high-throughput screening of newly developed drugs. Methods Modified media without yeast extract were evaluated for their ability to support the growth of T. vaginalis parasites. The potential of the nucleic acid-binding dye SYBR Green I for detecting T. vaginalis drug resistance was characterized, and seeding parasite concentration and incubation time were optimized. The fluorescence assay based on SYBR Green I was further validated in four T. vaginalis isolates with different susceptibilities to the antibiotics metronidazole, tinidazole, ornidazole and secnidazole, and compared with the traditional method that detects minimum lethal concentrations (MLCs). Results A modified medium consisting of RPMI 1640 and Tryptone Plus as replacements for yeast extract and tryptone, respectively, in traditional trypticase-yeast extract-maltose (TYM) medium exhibited similar performance as TYM medium in maintaining T. vaginalis growth, while it showed much lower background fluorescent signals. The T. vaginalis SYBR Green I-based fluorescence (TSF) drug assay was found to have to satisfy one of two conditions to demonstrate the 50% inhibitory concentration of metronidazole for the sensitive isolate TV-334: (i) a seeding density of 3 × 104 parasites/ml and an incubation time of 48 h; or (ii) a seeding density of 1 × 104 parasites/ml and an incubation time of 72 h. Subsequent validation experiments revealed that the 48-h incubation/3 × 104 parasites/ml seeding density condition had a greater sensitivity to detect drug resistance than the 72-h condition. The TSF assay also exhibited high efficiency in identifying parasite drug resistance, as evidenced by its strong correlation with the standard MLC assay results (P = 0.003). Conclusions This study presents a robust TSF assay that has the potential to facilitate high-throughput, automated in vitro anti-trichomoniasis susceptibility testing for drug resistance monitoring and drug development. In comparison to the standard MLC method, this assay offers the advantages of reduced labor and elimination of subjective examination. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

A fluorescence-based assay for Trichomonas vaginalis drug screening

Chen,

Li,

Wang

et al. 2023

Parasites Vectors

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Characterization of Actin-based Genotypes and Mycoplasma Endosymbionts of Trichomonas vaginalis Isolated in Sapporo, Japan

Yamazaki,

Sawai,

Takahashi

et al. 2024

Acta Parasit.

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Cell Surface Glycans as an Adhesion Factor for Exosomal Vesicles of Tritrichomonas Spp. Type Parasites

Bets,

Shilova,

Gribchenko

et al. 2024

Preprint

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Protozoal infection is a serious factor in the decline of reproductive health in humans and animals. The example of T. vaginalis shows how the parasite-host interaction is mediated. It is known that signaling between cells and parasites is carried out with the help of specific connections. These bonds can be represented by glycans and lectins. In this work, we examined the glycan profile of cells and lectin profile of exosomal vesicles of the intestinal trichomonad Tritrichomonas sp. in an intestinal epithelial model. The cytotoxicity of active trophozoites on cells of the CaCo-2 tumor cell line was shown, and the absence of cytotoxic effect of exosomal vesicles on cells was also demonstrated. We investigated the glycan profile of CaCo-2 cells by treatment with biotinylated lectins and found a high percentage of binding to the lectins RCA120, AAL, and UEA-1. These lectins bind galactose and fucose-containing glycans. When we determined the lectin profile of vesicles on glycan chips, we found vesicles bound to alpha-Gal beta-Gal and GalNAc, as well as Fuca1. We hypothesize that exosomal vesicles interact with the host cell through these glycan-lectin chains. By selecting blockers of these interactions, parasite adhesion can be inhibited. These mechanisms may form the basis of new antiprotozoal drugs.

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Prevalence and metronidazole resistance of Trichomonas vaginalis among Japanese women in 2021

Cited by 3 publications

References 29 publications

A fluorescence-based assay for Trichomonas vaginalis drug screening

A fluorescence-based assay for Trichomonas vaginalis drug screening

Characterization of Actin-based Genotypes and Mycoplasma Endosymbionts of Trichomonas vaginalis Isolated in Sapporo, Japan

Cell Surface Glycans as an Adhesion Factor for Exosomal Vesicles of Tritrichomonas Spp. Type Parasites

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