Prevalence and molecular characterisation of Sarcocystis miescheriana and Sarcocystis suihominis in wild boars (Sus scrofa) in Italy

Gazzonis, Alessia Libera; Gjerde, Bjørn; Villa, Luca; Minazzi, Stefano; Zanzani, Sergio Aurelio; Riccaboni, P.; Sironi, G.; Manfredi, Maria Teresa

doi:10.1007/s00436-019-06249-2

Cited by 39 publications

(41 citation statements)

References 34 publications

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“…aretiecanis 95.8–96.3 KU820984 ; MK420017 ( Hu et al, 2016 ; Gjerde et al, 2020 ) Sarcocystis sp. MT737952 Fox 16 Fox16 PCR 639 S. miescheriana 100 MH404232 Gazzonis et al (2019) S. miescheriana MT737934 Fox16 clone1-Fox16 clone8 664 S. miescheriana 99.9–100 MH404232 Gazzonis et al (2019) S. miescheriana MW020901- MW020903 Fox 46 Fox46 PCR 617 S. hjorti 100 GQ250990 Dahlgren and Gjerde (2010a) S. hjorti MT737935 Fox 47 Fox47 PCR 638 S. gracilis 100 MN334289 Rudaitytė-Lukošienė et al (2020) S. gracilis MT737936 Fox47 clone1-Fox47 clone4 675 S. gracilis 99.7–100 MN334289 Rudaitytė-Lukošienė et al (2020) S. gracilis MW020904- MW020907 Fox47 clone5 647 S. tenella …”

Section: Resultsmentioning

confidence: 99%

Sarcocystis infection in red deer (Cervus elaphus) with eosinophilic myositis/fasciitis in Switzerland and involvement of red foxes (Vulpes vulpes) and hunting dogs in the transmission

Basso

Rojas

Buob

et al. 2020

International Journal for Parasitology: Parasites and Wildlife

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Red deer ( Cervus elaphus ) carcasses showing grey-greenish discolouration have been increasingly observed in the canton of Grisons, Switzerland. We investigated whether Sarcocystis infections were associated with this pathology, and whether wild and domestic canids were involved in their transmission. Meat from affected red deer ( n = 26), faeces and intestines from red foxes ( Vulpes vulpes ) ( n = 126), and faeces from hunting dogs ( n = 12) from the region, were analysed. Eosinophilic myositis and/or fasciitis were diagnosed in 69% of the deer, and sarcocysts were observed in 89% of the animals. Molecular typing targeting a ~700bp variable region of the 18S rRNA gene revealed Sarcocystis hjorti in 73%, S. venatoria/S. iberica in 54%, S. linearis/S. taeniata in 12%, S. pilosa in 8% and S. ovalis in 4% of the deer samples. No inflammatory changes were observed in red deer carcasses with normal appearance ( n = 8); however, sarcocysts were observed in one sample, and S. hjorti , S. venatoria/S. iberica or S. silva DNA was detected in five samples. Sarcocystis oocysts/sporocysts were observed in 11/106 faecal and 6/20 intestinal fox samples, and in 2/12 canine samples. Sarcocystis tenella ( n = 8), S. hjorti ( n = 2), S. gracilis ( n = 2), and S. miescheriana ( n = 1) were identified in foxes, and S. gracilis ( n = 2), S. capreolicanis ( n = 1) and S. linearis/S. taeniata ( n = 1) in dogs. This study provides first molecular evidence of S. pilosa and S. silva infection in red deer and S. linearis/S. taeniata in dogs and represents the first record of S. ovalis transmitted by corvids in Central Europe. Although Sarcocystis species infecting red deer are not regarded as zoonotic, the affected carcasses can be declared as unfit for human consumption due to the extensive pathological changes.

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Section: Resultsmentioning

confidence: 99%

Sarcocystis infection in red deer (Cervus elaphus) with eosinophilic myositis/fasciitis in Switzerland and involvement of red foxes (Vulpes vulpes) and hunting dogs in the transmission

Basso

Rojas

Buob

et al. 2020

International Journal for Parasitology: Parasites and Wildlife

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“…Sarcocystis spp. isolated from C. dromedarius from Dammam and Riyadh grouped alongside S. levinei hosted by B. bubalis (heart tissue) in India (accession numbers MH255774-MH255777) [42], S. rangi hosted by R. tarandus in Norway (accession numbers KC209662-KC209666) [43], S. miescheriana hosted by S. scrofa in Italy (accession number MH404202) [44], and S. fayeri hosted by E. caballus in Canada (accession number LC171854) [45].…”

Section: Discussionmentioning

confidence: 99%

Identification of Sarcocystis spp. in One-humped Camels (Camelus dromedarius) from Riyadh and Dammam, Saudi Arabia, via Histological and Phylogenetic Approaches

Metwally

Al-Otaibi

Al-Turaiki

et al. 2020

Animals

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Sarcocystis (S.) spp. are intracellular protozoan parasites that infect birds and animals, resulting in substantial commercial losses. Sarcocystis spp. have an indirect life cycle; canines and felines are known to act as final hosts, and numerous domestic and wild animals act as intermediate hosts. The presence of sarcocysts in camel meat may diminish its commercial quality. There is limited knowledge regarding the taxonomy and diagnosis of Sarcocystis spp. that infect camels in Saudi Arabia. In this study, transmission electron microscopy (TEM) revealed S. cameli and S. camelicanis (camelicanis) in Camelus (C.) dromedarius. This is the first report of S. camelicanis in Saudi Arabia and is considered a significant finding. Based on cytochrome c oxidase subunit I gene (COX1) sequences, two samples of Sarcocystis spp. isolated from C. dromedarius in Riyadh and Dammam were grouped with S. levinei hosted by Bubalus bubalis in India, S. rangi hosted by Rangifer tarandus in Norway, S. miescheriana hosted by Sus scrofa in Italy and S. fayeri hosted by Equus caballus in Canada. The sequences obtained in this study have been deposited in GenBank.

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“…Furthermore, serological studies on the spread of N. caninum were also conducted in European wild boars' populations (Almería et al 2007;Bártová et al 2006;Reiterová et al 2016). Considering that wild boar populations are nowadays in expansion in terms both of the number of animals and habitat range, the increased frequency of contacts among wild boars, livestock, and humans could influence the transmission of zoonotic and animal-specific pathogens, as previously demonstrated in this area for other similar protozoa infections (Gazzonis et al 2018b(Gazzonis et al , 2019a. In the same study area, a recent molecular survey reporting the presence of N. caninum in brain tissue of wild birds of prey not only suggests the involvement of avian species in the parasite life cycle but also the environmental circulation of Neospora, indicating a possible role of these wild populations in the epidemiology of the parasite infection at the interface of the domestic and sylvatic life cycle (Gazzonis et al 2021).…”

Section: Discussionmentioning

confidence: 93%

Prevalence of Neospora caninum antibodies in fattening pigs and sows from intensive farms in northern Italy

et al. 2022

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Neospora caninum (Apicomplexa, Sarcocystidae) is a major cause of reproductive failure in cattle. In pigs, only a few studies investigated the effects of this parasite on reproductive efficiency. Considering the relevance of swine farms in northern Italian regions, an epidemiological survey was designed to investigate the spread of N. caninum infection. Three hundred seventy fattening pigs and sows from 23 intensive farms in Lombardy were sampled. Sera were analyzed by a commercial immunofluorescence antibody test. Statistical analysis through univariate and multivariate generalized linear models was conducted to detect farm management practices enhancing the risk of infection. At the farm level, 52.1% (12/23) of the selected farms, 72.7% housing sows and 40% fattening pigs, scored positive. At the individual level, 25 animals (25/370, P = 6.7%) were positive to N. caninum antibodies: one fattening pig and two sows showed an antibody titer of 1:100, and in two sows, an antibody titer of 1:400 and 1:6400 was evidenced. A higher seroprevalence was detected in sows (17/151, P = 11.2%) if compared to fattening pigs (8/219, P = 3.6%) (OR = 1.19, P value = 0.000 in sows). Moreover, a higher seroprevalence was recorded in farms with low and moderate sanitary score (P = 100% and P = 64.2%, respectively) if compared to farms with high sanitary score (P = 22.2%) (OR = 1.24, P value = 0.007 in score = 1 and OR = 1.10, P value = 0.050 in score = 2). This study provides the first data on the circulation of N. caninum in intensive swine farms in Italy, demonstrating the spread of the parasite in fattening pigs and sows in Lombardy region.

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Prevalence and molecular characterisation of Sarcocystis miescheriana and Sarcocystis suihominis in wild boars (Sus scrofa) in Italy

Cited by 39 publications

References 34 publications

Sarcocystis infection in red deer (Cervus elaphus) with eosinophilic myositis/fasciitis in Switzerland and involvement of red foxes (Vulpes vulpes) and hunting dogs in the transmission

Sarcocystis infection in red deer (Cervus elaphus) with eosinophilic myositis/fasciitis in Switzerland and involvement of red foxes (Vulpes vulpes) and hunting dogs in the transmission

Identification of Sarcocystis spp. in One-humped Camels (Camelus dromedarius) from Riyadh and Dammam, Saudi Arabia, via Histological and Phylogenetic Approaches

Prevalence of Neospora caninum antibodies in fattening pigs and sows from intensive farms in northern Italy

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