Prevalence and molecular characterization of Hepatozoon canis in dogs from urban and rural areas in Southeast Brazil

Miranda, Renata Lima de; O’Dwyer, Lúcia Helena; Castro, Jairo Lopes de; Metzger, Betina; Rubini, Adriano Stefani; Mundim, Antônio Vicente; Eyal, Osnat; Talmi-Frank, Dalit; Cury, Márcia Cristina; Baneth, Gad

doi:10.1016/j.rvsc.2014.06.015

Cited by 35 publications

(39 citation statements)

References 16 publications

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“…Hepatozoon canis has been detected in dogs in several regions in Brazil, with frequencies ranging from 0.48% (1/205) (RAMOS et al, 2010) to 75.9% (161/212) (MIRANDA et al, 2014) in dogs from urban areas of Recife and Uberlândia, respectively. In a single survey conducted in Campo Grande, a Hepatozoon spp.…”

Section: Resultsmentioning

confidence: 99%

“…Although the methodology used in the present study (PCR) is more sensitive than optical microscopy, and the current sampling has been performed in sick dogs, in which a higher frequency of infected animals would be expected, no relevant difference was observed with respect to the previous study (SALGADO, 2006). On the other hand, a comparative analysis of data from different regions of Brazil revealed significant differences in H. canis infection rates in urban areas (RAMOS et al, 2010;PEREIRA et al, 2011;MIRANDA et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

“…In Brazil, the brown dog tick Rhipicephalus sanguineus has been incriminated as the main vector of H. canis in urban areas (SPOLIDORIO et al, 2009;MIRANDA et al, 2014) because it is the most common tick in these areas (LABRUNA & PEREIRA, 2001;DANTAS-TORRES et al, 2004). However, an association has not always been observed between high levels of tick infestation and high frequency of H. canis infection in dogs (SPOLIDORIO et al, 2009;MIRANDA et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

“…The transmission of H. canis to dogs in urban areas has been attributed mainly to the tick species Rhipicephalus sanguineus (LATROFA et al, 2014;MIRANDA et al, 2014), while in the rural environment, Amblyomma ovale and Rhipicephalus (Boophilus) microplus have been incriminated (FORLANO et al, 2005;MIRANDA et al, 2011). However, differences in the vector competence of these ticks can influence the prevalence of the parasite in different dog populations (SILVA et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification of Hepatozoon canis in dogs from Campo Grande, Mato Grosso do Sul, Brazil

Ramos

Babo-Terra

Pedroso

et al. 2015

Rev. Bras. Parasitol. Vet.

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The aim of this study was to investigate the occurrence of Hepatozoon species infecting dogs in the municipality of Campo Grande, Mato Grosso do Sul (MS), Brazil, using blood samples (n = 165) drawn from dogs. The species Hepatozoon canis was identified in 3.63% of the tested animals using molecular tools. Further studies are needed to determine the clinical relevance of this infection and the main arthropod vectors involved in its transmission.Keywords: Dogs, epidemiology, Hepatozoon canis, midwestern Brazil, PCR. ResumoO objetivo deste estudo foi identificar a frequência e espécies de Hepatozoon infectando cães no município de Campo Grande, Mato Grosso do Sul, Brasil. Uma amostragem de 165 animais foi utilizada e, por meio do uso de ferramentas moleculares, a espécie Hepatozoon canis foi identificada em 3,63% dos animais. Mais estudos são necessários para identificar a relevância clínica e os principais vetores envolvidos na transmissão desse protozoário na região.Palavras-chave: Cães, epidemiologia, Hepatozoon canis, centro-oeste do Brasil, PCR.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular identification of Hepatozoon canis in dogs from Campo Grande, Mato Grosso do Sul, Brazil

Ramos

Babo-Terra

Pedroso

et al. 2015

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

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“…could be due to many factors including the distribution and population density of the vector (Otranto et al 2011), the sampling methodology and the characteristics of the targeted dog population (Gomes et al 2010;De Miranda et al 2014). An analysis of risk factors indicated that male dogs were more often infected with E. canis than the females, but this association was statistically non-significant (P = 0.15) confirming previous studies reporting that there is no correlation between the sex and the presence of E. canis infection in dogs (Rani et al 2011;Aktas et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of Ehrlichia canis in dogs from three districts in Punjab (Pakistan)

Malik

Qamar

Ain

et al. 2018

Veterinary Medicine & Sci

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Canine monocytic ehrlichiosis is a tick-borne disease caused by an intracellular alpha-proteobacterium, Ehrlichia canis, which replicates within mononuclear cells in the host. This study was designed to use a polymerase chain reaction (PCR) protocol for the molecular detection of E. canis by the amplification of a portion of its 16S rRNA gene, as well as the effects of this alpha-proteobacterium on the haematological parameters of the sampled dogs and the risk factors associated with E. canis infection. A total of 151 blood samples were collected from dogs of various breeds at three sampling sites (Lahore, Rawalpindi/Islamabad and Multan) in Punjab, Pakistan. Data regarding the epidemiological factors (including age, gender, breed, body temperature, deworming, vaccination, mucous membrane status, hydration status, the presence of haematuria and tick infestation) were collected through a questionnaire at the time of sample collection. A 400 bp DNA fragment of the 16S rRNA gene of E. canis was amplified from 42 dog blood samples (28% of the total), [Lahore (N = 24), Rawalpindi/Islamabad (N = 13) and Multan (N = 05)] through PCR. Data analysis revealed that the character of the animals (age, sex and breed) had no significant association (P > 0.05) with the presence of E. canis. Various haematological parameters were also compared, and the results revealed that all of the parameters remained unaffected, except significantly lower white blood cell counts (P = 0.004) in E. canis-positive blood samples, as compared with the control group. We concluded that this is the first molecular confirmation of canine infection by E. canis using PCR. Moreover, no specific epidemiological parameter was found associated with the prevalence of E. canis in dogs.

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Molecular characterization of Hepatozoon canis from farm dogs in Pakistan

et al. 2018

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Hepatozoon canis is a tick-borne pathogen of canids, which is distributed worldwide. However, very little is known about this protozoan parasite in Pakistan. This study provides the first molecular evidence of H. canis from farm dogs from three agro-ecological zones of Punjab, Pakistan. A conventional PCR targeting the 18S rRNA gene was used to characterize H. canis from farm dogs from three districts, namely Kasur, Rawalpindi, and Muzaffargarh, in Punjab. Of 341 blood samples tested, 155 (45.5%) were positive for H. canis, 73 (61.3%) from Kasur, 46 (42.5%) from Rawalpindi, and 36 (31.5%) from Muzaffargarh. Phylogenetic analyses revealed that 18S rRNA sequences of H. canis from this study clustered in three clades with those of H. canis from previously published studies to the exclusion of all other Hepatozoon spp. included in the analysis. This study provides the first insight into H. canis from farm dogs in Pakistan. Furthermore, it lays a foundation for future studies of the parasite to assess the impact of canine hepatozoonosis in dogs from various agro-ecological zones in Pakistan where pet ownership of dogs is increasing.

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Prevalence and molecular characterization of Hepatozoon canis in dogs from urban and rural areas in Southeast Brazil

Cited by 35 publications

References 16 publications

Molecular identification of Hepatozoon canis in dogs from Campo Grande, Mato Grosso do Sul, Brazil

Molecular identification of Hepatozoon canis in dogs from Campo Grande, Mato Grosso do Sul, Brazil

Molecular detection of Ehrlichia canis in dogs from three districts in Punjab (Pakistan)

Molecular characterization of Hepatozoon canis from farm dogs in Pakistan

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