Prevalence and molecular features of ESBL/pAmpC-producing Enterobacteriaceae in healthy and diseased companion animals in Brazil

Melo, Luana C.; Oresco, Cíntia; Leigue, Lucianne; Netto, Hildebrando M.; Melville, Priscilla Anne; Benites, N. R.; Saras, Estelle; Haenni, Marisa; Lincopán, Nilton; Madec, Jean‐Yves

doi:10.1016/j.vetmic.2018.05.017

Cited by 56 publications

(49 citation statements)

References 47 publications

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“…Companion animals could play a role as a reservoir of AMR bacteria, as they are in close association with humans, living in their homes, and near their food. In addition, the widespread use and misuse of antibiotics in both human and veterinary medicine is increasing the spread of AMR bacteria (Dorado-García et al, 2018; Melo et al, 2018; Pulss et al, 2018). However, the impact of companion animals on human health in terms of attributing to exchange and share AMR determinant is not yet clear.…”

Section: Introductionmentioning

confidence: 99%

Clonal Spread of Extended-Spectrum Cephalosporin-Resistant Enterobacteriaceae Between Companion Animals and Humans in South Korea

et al. 2019

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Extended-spectrum cephalosporin (ESC)-resistant Enterobacteriaceae is an increasingly important problem in both human and veterinary medicine. The aims of this study were to describe a comparative molecular characterization of Enterobacteriaceae carrying ESC resistance genes, encoding extended-spectrum β-lactamase (ESBL) and AmpC, isolated from human stool samples, rectal swabs from companion animals, and swabs from the environment of veterinarian hospitals in South Korea, and to examine their possible dissemination and transmission. The ESC resistance genes were identified by PCR and sequencing. Isolates with the predominant ESC resistance genes were assessed for their genetic relatedness by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing. A total of 195 Escherichia coli and 41 Klebsiella pneumoniae isolates that exhibited ESC resistance were recovered on CHROMagar ESBL from human, companion animal, and the veterinary hospital environmental samples. In companion animals, most of the ESC resistance genes were bla CMY–2–like (26.4%), followed by bla CTX –M–55 (17.2%) and bla CTX–M–14 (16.1%), whereas bla CTX–M–15 (28.6%) was predominant in human samples. The epidemiological relatedness of isolates carrying ESC resistance genes, including 124 E. coli and 23 K. pneumoniae isolates carrying CMY-2-like, DHA-1-like, or/and CTX-M-type, were analyzed by PFGE. The pulsotypes of five E. coli isolates (three from dogs and two from humans) carrying bla CMY–2–like , which were attributed to sequence type 405, from different veterinary clinics showed >85% similarity. Our results indicate direct transmission and dissemination of ESC-resistant Enterobacteriaceae between humans and companion animals.

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Section: Introductionmentioning

confidence: 99%

Clonal Spread of Extended-Spectrum Cephalosporin-Resistant Enterobacteriaceae Between Companion Animals and Humans in South Korea

et al. 2019

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“…Infections caused by ESBL-PE in dogs and cats include abscesses and wounds, otitis, upper respiratory tracts diseases, gastro-intestinal infections and cystitis [ 8 , 10 ]. Colonization was also described worldwide, with rates ranging from 6% to 24% in different geographical regions and different cohorts [ 11 , 12 , 13 , 14 ]. Recent studies described co-carriage of ESBL producing Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) strains in humans and dogs of the same household [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Extended-Spectrum β-Lactamase-Producing Enterobacterales Shedding by Dogs and Cats Hospitalized in an Emergency and Critical Care Department of a Veterinary Teaching Hospital

et al. 2020

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Extended-spectrum β-lactamase-producing Enterobacterales (ESBL-PE) gut shedding in human medicine is considered as a major reservoir for ESBL-associated infections in high risk patients. In veterinary medicine, data regarding ESBL-PE gut shedding on admission to emergency and critical care department is scarce. We aimed to determine ESBL-PE shedding rates by dogs and cats in this setting and to determine the risk factors for shedding, at two separate periods, three-years apart. Rectal swabs were collected from animals, on admission and 72 h post admission, enriched and plated on Chromagar ESBL plates, followed by bacterial identification. ESBL phenotype was confirmed and antibiotic susceptibility profiles were determined (Vitek 2). Medical records were reviewed for risk factor analysis (SPSS). Overall, 248 animals were sampled, including 108 animals on period I (2015–2016) and 140 animals on period II (2019). In both periods combined, 21.4% of animals shed ESBL-PE on admission, and shedding rates increased significantly during hospitalization (53.7%, p-value < 0.001). The main ESBL-PE species were Escherichia coli and Klebsiella pneumoniae, accounting for more than 85% of the isolates. In a multivariable analysis, previous hospitalization was a risk factor for ESBL-PE gut shedding (p-value = 0.01, Odds ratio = 3.05, 95% Confidence interval 1.28–7.27). Our findings demonstrate significant ESBL-PE gut shedding among small animals in the emergency and critical care department, posing the necessity to design and implement control measures to prevent transmission and optimize antibiotic therapy in this setting.

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“…Furthermore, plasmid-mediated AmpC (pAmpC) were identified from Enterobacteriaceae such as, Klebsiella spp., Escherichia coli, Salmonella spp., and Proteus mirabilis [17]. The distribution of pAmpC seems to be more frequent in animals than in humans [16]. Although ESBL/AmpC-producing Klebsiella spp.…”

Section: Introductionmentioning

confidence: 99%

“…Extended-spectrum β-lactamase-producing Enterobacteriaceae have gained special attention on AMR in horses due to their presence as a potentially zoonotic bacteria [14]. The CTX-M family of ESBL have been reported as the predominant type of ESBL after the TEM and SHV types [15], and more than 200 CTX-M variants have been identified worldwide [16]. On the other hand, some species of Enterobacteriaceae (such as Enterobacter cloacae, Enterobacter aerogenes, Aeromonas sp., Citrobacter freundii, Providencia sp., Serratia marcescens, Hafnia alvei, Morganella morganii, and Pseudomonas aeruginosa) have resistance to extended-spectrum cephalosporin, which may be caused by inducible chromosomal AmpC.…”

Section: Introductionmentioning

confidence: 99%

Multidrug-Resistant ESBL/AmpC-Producing Klebsiella pneumoniae Isolated from Healthy Thoroughbred Racehorses in Japan

Sukmawinata

Uemura

Sato

et al. 2020

Animals

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Extended-spectrum β-lactamase (ESBL)- and AmpC β-lactamase (AmpC)-producing Klebsiella spp. have become a major health problem, leading to treatment failure in humans and animals. This study aimed to evaluate the presence of ESBL/AmpC-producing Klebsiella spp. isolated from racehorses in Japan. Feces samples from 212 healthy Thoroughbred racehorses were collected from the Japan Racing Association Training Centers between March 2017 and August 2018. ESBL/AmpC-producing Klebsiella spp. were isolated using selective medium containing 1 µg/mL cefotaxime. All isolates were subjected to bacterial species identification (MALDI-TOF MS), antimicrobial susceptibility test (disk diffusion test), characterization of resistance genes (PCR), conjugation assay, and genetic relatedness (multilocus sequence typing/MLST). Twelve ESBL/AmpC-producing Klebsiella pneumoniae (ESBL/AmpC-KP) were isolated from 3.3% of horse samples. Antimicrobial resistance profiling for 17 antimicrobials showed all ESBL/AmpC-KP were multidrug-resistant (MDR). Only 1 isolate was confirmed as an ESBL producer (blaCTX-M-2-positive), whereas the other 11 isolates were plasmid-mediated AmpC (pAmpC) producers (blaCMY positive). On the basis of MLST analysis, the ESBL-KP isolate was identified as sequence type (ST)-133 and four different STs among AmpC-KP isolates, ST-145, ST-4830, ST-4831, and ST-4832, were found to share six of the seven loci constituting a single-locus variant. This is the first study to show K. pneumoniae carrying MDR pAmpC isolated from a racehorse.

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Prevalence and molecular features of ESBL/pAmpC-producing Enterobacteriaceae in healthy and diseased companion animals in Brazil

Cited by 56 publications

References 47 publications

Clonal Spread of Extended-Spectrum Cephalosporin-Resistant Enterobacteriaceae Between Companion Animals and Humans in South Korea

Clonal Spread of Extended-Spectrum Cephalosporin-Resistant Enterobacteriaceae Between Companion Animals and Humans in South Korea

Extended-Spectrum β-Lactamase-Producing Enterobacterales Shedding by Dogs and Cats Hospitalized in an Emergency and Critical Care Department of a Veterinary Teaching Hospital

Multidrug-Resistant ESBL/AmpC-Producing Klebsiella pneumoniae Isolated from Healthy Thoroughbred Racehorses in Japan

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