Prevalence and molecular identification of Cryptosporidium spp in cattle in Baghdad province, Iraq

Alseady, H.H.; Kawan, May H.

doi:10.33899/ijvs.2019.163084

Cited by 8 publications

(6 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Diagnosis of Cryptosporidium spp. is difficult due to their oocysts size when compared with another coccidian, therefore is difficult to observe, and the resemblance same to spores of yeast and fungi (10). Many of laboratory methods for diagnosis parasite infection have many limitations as acid fast stain or microscopic examination.…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of Cryptosporidium parvum in chicken in Al-Diwaniya province

Jarad¹

2020

IJVS

View full text Add to dashboard Cite

This study were conducted in Al-Diwaniya province, in south Iraq during the period from February to July 2019 to determine the rate of infection of Cryptosporidium parvum in domestic chicken, study the effect of some epidemiological factors such as sex and months on the rate of infection, addition to the molecular identification of Cryptococcus parvum by amplification HSP70 gene by conventional PCR. Number of collected fecal sample was 210 from domestic chicken and stained by Ziehl-Neelsen stain. The results of the microscopic examination showed that 108(51.4%) out of 210 fecal samples were infected with Cryptosporidium spp. The statistical analysis founded no marked difference in prevalence of infection between sexes. Significant difference was recorded between infection rate during the months of the study and higher prevalence of infection rate was observed in March 11.9%, while lowest infection rate was observed in July 5.23%. and June 5.23%. Genomic DNA was extracted from 108 fecal samples and HSP70 gene for C. parvum was amplified by PCR. PCR technique is showed that out of 108 fecal samples 21.3% were positive for Cryptosporidium parvum.

show abstract

Section: Introductionmentioning

confidence: 99%

Molecular detection of Cryptosporidium parvum in chicken in Al-Diwaniya province

Jarad¹

2020

IJVS

View full text Add to dashboard Cite

show abstract

“…However, found the lowest level in cattle between 32-44.4% (10,18), while in another study, the overall prevalence of N. caninum infection was 46.9% (19). Perhaps the difference is due to the geographical area or the surrounding environment and topographical reasons that affect the development and survival of oocysts (20). Many factors may affect the seroprevalence of parasites including sample size, environmental differences age and breed of cattle (21,22).…”

Section: Discussionmentioning

confidence: 92%

Seroprevalence of Neospora caninum infections in cattle in Kirkuk province

Nooraldin¹,

Jaafar²,

Salih³

2021

IJVS

View full text Add to dashboard Cite

Neospora caninum was identified as a coccidian parasite as in 1988, before that date it was misclassified as Toxoplasma gondii based on structural similarities. The aim of this study was to determine the seroprevalence of N. caninum in cattle in Kirkuk Province. A total of 185 cattle serum samples were collected randomly. Data about ages, gender and abortion history of females were recorded. Serum samples were examined by using ELISA as a screening test for the detection of anti-N. caninum IgG antibodies of past infection. Results showed that 53 (28.6%) cattle were seropositive infected, with significantly higher seropositive infection of age group >2 years in comparison to other age groups 94.3%. The distribution of N. caninum of total infected cattle showed that the seropositive infection in females were 47(88.7%), which was more than males 6 (11.3%) with a highly significant difference. There were 22(48.8%) aborted cattle out of 47 infected cattle with N. caninum with no significant difference in comparison with the healthy group.

show abstract

“…Similarly, Clarke and McIntyre reported that false-positive samples were detected by microscopy because of the presence of artifacts, such as yeasts and debris in the stool; however, false-negative samples were also found to be due to poor uptake of stain by the oocytes (36). Furthermore, by Alseady revealed that; the overall prevalence of infection with Cryptosporidium is 21% (21/100) by conventional microscopic (modified Ziehl-Neelsen staining) method on the other hand, PCR diagnostic technique the Cryptosporidium infection is detected in 38 samples 38% with sensitivity 100% which the differences of infection attributed to multiple factors included management systems and rearing methods, non modified risk factors likes' age, environmental conditions and breed of cattle and modified risk factors as, the sampling techniques and diagnostic methods (37). The most common species of Cryptosporidiosis are C. parvum in rural area then C. hominis in urban area and the lowest is C. ryana and C. bovis and also there are fact that C. parvum is not specific to a host (38).…”

Section: Discussionmentioning

confidence: 99%

Detection of Cryptosporidium parvum in calf feces using microscopical, serological, and molecular methods

Felefel¹,

Abdel-Rady²,

El-Rahim³

et al. 2023

IJVS

View full text Add to dashboard Cite

Cryptosporidiosis is a critical gastrointestinal disease in calves. This study examined 150 fecal samples of diarrheic calves collected from the eastern region of Saudi Arabia for detection of Cryptosporidium parvum using the Modified Ziehl-Neelsen (MZN) method, Enzyme-Linked Immunosorbent Assay (ELISA), and conventional Polymerase Chain Reaction (PCR). The performance of these methods was assessed using diagnostic accuracy tests. The present study identified C. parvum oocysts in fecal samples by modified Ziehl-Neelsen 40/150; 26.66%, ELIZA 60/150; 40%, and PCR 78/150; 52% methods, respectively. The microscopic method revealed higher specificity 65.27% than the ELIZA 51.38%, while the ELIZA showed higher sensitivity 32.05% than MZN method 19.23%. However, MZN and ELIZA methods were unsatisfactory diagnostic tools compared with the PCR as the area under the curve values in Receiver Operator Characteristic (ROC) analysis were less than 0.6. Furthermore, using the kappa analysis test revealed no agreement between MZN and ELISA methods compared with PCR at P<0.05.

show abstract

Prevalence and molecular identification of Cryptosporidium spp in cattle in Baghdad province, Iraq

Cited by 8 publications

References 19 publications

Molecular detection of Cryptosporidium parvum in chicken in Al-Diwaniya province

Molecular detection of Cryptosporidium parvum in chicken in Al-Diwaniya province

Seroprevalence of Neospora caninum infections in cattle in Kirkuk province

Detection of Cryptosporidium parvum in calf feces using microscopical, serological, and molecular methods

Contact Info

Product

Resources

About