Prevalence and pathogenic potential of Arcobacter spp. isolated from edible bivalve molluscs in Sardinia

Mudadu, Alessandro Graziano; Salza, Sara; Melillo, Rita; Ladu, Mara; Piras, Gabriella; Spanu, Carlo; Terrosu, G.; Fadda, Amedeo; Virgilio, Sebastiano; Tedde, T.

doi:10.1016/j.foodcont.2021.108139

Cited by 12 publications

(7 citation statements)

References 54 publications

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“…Though little is published about the Pseudoarcobacter genus, the Arcobacter genus has been identified in other shellfish including as a common commensal of the oyster microbiota [56,66,67]. Arcobacter also includes several species known to be pathogenic to humans [68][69][70]. The relative abundance of Arcobacteriaceae in the oyster microbiome was higher in the colder than the warmer months ( [71].…”

Section: Plos Onementioning

confidence: 99%

Analysis of the core bacterial community associated with consumer-ready Eastern oysters (Crassostrea virginica)

et al. 2023

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Shellfish, such as the Eastern oyster (Crassostrea virginica), are an important agricultural commodity. Previous research has demonstrated the importance of the native microbiome of oysters against exogenous challenges by non-native pathogens. However, the taxonomic makeup of the oyster microbiome and the impact of environmental factors on it are understudied. Research was conducted quarterly over a calendar year (February 2020 through February 2021) to analyze the taxonomic diversity of bacteria present within the microbiome of consumer-ready-to-eat live Eastern oysters. It was hypothesized that a core group of bacterial species would be present in the microbiome regardless of external factors such as the water temperature at the time of harvest or post-harvesting processing. At each time point, 18 Chesapeake Bay (eastern United States) watershed aquacultured oysters were acquired from a local grocery store, genomic DNA was extracted from the homogenized whole oyster tissues, and the bacterial 16S rRNA gene hypervariable V4 region was PCR-amplified using barcoded primers prior to sequencing via Illumina MiSeq and bioinformatic analysis of the data. A core group of bacteria were identified to be consistently associated with the Eastern oyster, including members of the phyla Firmicutes and Spirochaetota, represented by the families Mycoplasmataceae and Spirochaetaceae, respectively. The phyla Cyanobacterota and Campliobacterota became more predominant in relation to warmer or colder water column temperature, respectively, at the time of oyster harvest.

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Section: Plos Onementioning

confidence: 99%

Analysis of the core bacterial community associated with consumer-ready Eastern oysters (Crassostrea virginica)

et al. 2023

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“…Antibiotics are delivered to water also with feces from coastlines, which are usually densely populated. The figure is prepared based on literature ( Krieger et al, 1981 ; Kueh and Chan, 1985 ; Moy and Walday, 1996 ; Islam and Tanaka, 2004 ; Li et al, 2012 ; Weis, 2014 ; Hsu and Lee, 2015 ; Beaudry et al, 2016 ; Zannella et al, 2017 ; Chiesa et al, 2018 ; Kolm et al, 2018 ; Lopez-Joven et al, 2018 ; MacFadden et al, 2018 ; Bondarczuk and Piotrowska-Seget, 2019 ; Fresia et al, 2019 ; Hoegh-Guldberg et al, 2019 ; Pinsky et al, 2019 ; Chen et al, 2020 ; Grabowska et al, 2020 ; Martinez-Albores et al, 2020 ; Musella et al, 2020 ; Preena et al, 2020 ; Verga et al, 2020 ; Albano et al, 2021 ; Mudadu et al, 2021 ; Pepi and Focardi, 2021 ; Bagi and Skogerbø, 2022 ; Baker et al, 2022 ; Kamermans et al, 2022 ).…”

Section: Anthropogenic Impact On Marine Microbiotamentioning

confidence: 99%

“…These microorganisms may still be active when the molluscs are eaten raw. Bivalve molluscs, including blue mussels ( Mytilus edulis ), great scallops ( Pecten maximus ), flat oysters ( Ostrea edulis ), horse mussels ( Modiolus modiolus ), and carpet shells ( Mya arenaria ), are suspension feeders and actively filter and retain particles from their surrounding water, including free-living or particle-bound bacteria ( Kueh and Chan, 1985 ; Romalde et al, 2014 ; Hsu and Lee, 2015 ; Grevskott et al, 2016 , 2017 ; Zannella et al, 2017 ; Mudadu et al, 2021 ). Bivalves and the blue mussel ( Mytilus edulis ) are successfully used as indicator organisms for marine pollution monitoring.…”

Section: Introductionmentioning

confidence: 99%

Molluscs—A ticking microbial bomb

Kijewska

Koroza²,

Grudlewska‐Buda³

et al. 2023

Front. Microbiol.

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Bivalve shellfish consumption (ark shells, clams, cockles, and oysters) has increased over the last decades. Following this trend, infectious disease outbreaks associated with their consumption have been reported more frequently. Molluscs are a diverse group of organisms found wild and farmed. They are common on our tables, but unfortunately, despite their great taste, they can also pose a threat as a potential vector for numerous species of pathogenic microorganisms. Clams, in particular, might be filled with pathogens because of their filter-feeding diet. This specific way of feeding favors the accumulation of excessive amounts of pathogenic microorganisms like Vibrio spp., including Vibrio cholerae and V. parahaemolyticus, Pseudomonas aeruginosa, Escherichia coli, Arcobacter spp., and fecal coliforms, and intestinal enterococci. The problems of pathogen dissemination and disease outbreaks caused by exogenous bacteria in many geographical regions quickly became an unwanted effect of globalized food supply chains, global climate change, and natural pathogen transmission dynamics. Moreover, some pathogens like Shewanella spp., with high zoonotic potential, are spreading worldwide along with food transport. These bacteria, contained in food, are also responsible for the potential transmission of antibiotic-resistance genes to species belonging to the human microbiota. Finally, they end up in wastewater, thus colonizing new areas, which enables them to introduce new antibiotic-resistance genes (ARG) into the environment and extend the existing spectrum of ARGs already present in local biomes. Foodborne pathogens require modern methods of detection. Similarly, detecting ARGs is necessary to prevent resistance dissemination in new environments, thus preventing future outbreaks, which could threaten associated consumers and workers in the food processing industry.

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“…Although food safety is not to be compromised, the unnecessary use of animal testing in safety assessments and the increased demand from consumers for foods that have not been animal tested (specifically vegan food) may trigger an issue of laboratory animal welfare. The estimated prevalence was 13% and at least one virulence gene was detected in 58% of strains (Mudadu et al, 2021). Our French IHI Platform Team wished to take stock of this genus that is not well known and recently clarified within its taxonomy (Perez-Cataluna et al, 2018).…”

Section: Eren-netherlandmentioning

confidence: 99%

EFSA's activities on emerging risks in 2021

Gkrintzali,

Georgiev,

Matas

et al. 2023

EFS3

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The main objectives of EFSA's activities on emerging risks are: (i) to carry out activities to identify emerging risks; (ii) to develop and improve emerging risk identification (ERI) methodologies and approaches; and iii) to communicate on identified issues and risks. The outcome of these activities allows EFSA to prepare for future risk assessment challenges. EFSA networks of knowledge contributing to the ERI activity include the Emerging Risks Exchange Network, the Stakeholder Discussion Group on Emerging Risks, EFSA's scientific units, scientific panels and the Scientific Committee and its working groups. The current technical report summarises the activities of all groups involved in the ERI procedure and the issues identified in the course of 2021; it also describes the methodologies being developed and the collaborative activities. In total, 18 potential emerging issues were discussed in 2021. Eight were concluded to be emerging risks. The potential issues were classified according to the hazard and/or driver identified. In more than half of the issues discussed in 2021, a change in consumer trends was identified as a driver. A better understanding of these trends is particularly relevant for EFSA to improve preparedness. In addition, in 2021 the Emerging Risks Exchange Network members presented and discussed a total of 35 signals/potential emerging issues identified by their own horizon‐scanning activities. Several Member States representatives shared their work on signal identification with the objective of collecting additional information that can be used for a better characterisation of the retrieved signals. Finally, several procurements are ongoing in EFSA to develop methodologies for ERI in specific areas of interest. The implementation of more effective and comprehensive methods to proactively collect, share and disseminate knowledge in the landscape of emerging risks is crucial for EFSA's preparedness risk assessment scheme.

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Prevalence and pathogenic potential of Arcobacter spp. isolated from edible bivalve molluscs in Sardinia

Cited by 12 publications

References 54 publications

Analysis of the core bacterial community associated with consumer-ready Eastern oysters (Crassostrea virginica)

Analysis of the core bacterial community associated with consumer-ready Eastern oysters (Crassostrea virginica)

Molluscs—A ticking microbial bomb

EFSA's activities on emerging risks in 2021

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