Prevalence and some risk factors of bovine heamotropic mycoplasma in Nineveh province - Iraq

Crimean-Congo hemorrhagic fever (CCHF) is described as a tick-borne viral zoonosis highly prevalent in Africa, Asia, Russia and the Balkans within the distribution range of ticks that belong to the genus Hyalomma. This research aimed to verify the seroprevalence of CCHF in cows employing Indirect enzyme-linked immunosorbent assay (I-ELISA) in Mosul city / Iraq, to examine some epidemiological risk factors related to the incidence of CCHF. From October 2019 -September 2020, one hundred eighty-four blood samples were taken from 3-8-year-old cattle of both sexes, from several management systems and origins, from various parts in Mosul city, 10 ml of blood was taken from the jugular vein in test tubes without anticoagulant to obtain the sera for I-ELISA test, while epidemiological data were obtained by interviewing the farm-owners. I-ELISA was applied to detect the antibodies of CCHF in the serum. The overall seroprevalence of CCHF in cows was 40/184 (21.7%). As for the risk factors associated with increased seroprevalence of the disease, an increase in seroprevalence rates was observed at ages 6, 7 and 8 years 30.5%, among females 26.3% and imported animals 27.5%. which showed that the serological prevalence in indoor feeding and outdoor feeding, was not significantly different (p<0.05). This study concluded that there is a higher seroprevalence of CCHF in Mosul city along with many risk factors related to its incidence.

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“…Statistical analysis was performed employing computed 2*2 tables in Epi-InfoTM ® 7 software version 7 (15).…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Crimean Congo Hemorrhagic Fever in cows by ELISA in Mosul city

Esmaeel¹,

Hussain²,

Altaliby³

2021

IJVS

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“…According to history, no former vaccinations against pestivirus such as BVDV and BDV for these farms had equipped. Carefully, the blood was drawn by vein puncture into sterile vacutainers without anti-coagulant for serum obtaining and transported icebag and kept at -20°C until assays [32,33].…”

Section: Animals Study Area and Samplingmentioning

confidence: 99%

Prevalence of Bovine Viral Diarrhea Virus in Sheep in Nineveh Province

Hussain,

ali,

ِAbdulla

et al. 2025

Egyptian Journal of Veterinary Sciences

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N UMEROUS studies have been conducted at the global level to evaluate the prevalence of the bovine viral diarrhea virus (BVDV) in various animals, especially ruminants. It becomes clear that one of the most important steps in preventing viral infections, is to designing multiple programs for control, such as vaccination, the detection of vectors and the identification of predisposing factors to infection. This work is a first pioneering study designed to detect the infection rate of BVDV utilizing indirect ELISA and PCR technique, and to clarify the role of some risk related factors in sheep located in Nineveh province, Iraq. Between Dec. 2022 and July 2023, blood samples for this study were obtained from 184 sheep selected randomly represented 24 local and imported sheep flocks, from different regions of Nineveh province. The results were recorded that the infection rate of the disease in sheep was 16.84% and 2.71% based on the indirect ELISA and the conventional PCR technique respectively. Based on the multiplex PCR technique, three samples were BVDV-1genotypes. Significantly the large herd size and older age showed higher risk to BVDV, with no significant difference observed between origin of animals. This study indicates that the evidence of BVDV in sheep and their role in the epidemiology of the disease in cattle and maintenance of the virus. Therefore, the study of epidemiology and genetic diversity of the virus in sheep and goats is recommended.

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“…Hemoplasma bacteria have a significant impact on livestock outcome as it causes weight loss, reduces milk production, and leads to sudden death [ 12 ]. The zoonotic features of the disease cause hematological and neuroprogressive disorders in humans [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

First study on microscopic and molecular evidences of two bovine hemoplasma species in cattle herds in Al-Qadisiyah Province, Iraq

2022

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Background and Aim: Hemotropic Mycoplasmas are small epierythrocytic bacteria that cause infectious anemia in several livestock species and in humans. Several reports have been made on hemoplasma infections in the south and north of Iraq, but there have been no studies in the middle Euphrates of Iraq. This study aimed to evaluate the presence of hemoplasma species in cattle in Al-Qadisiyah Province, Iraq. Materials and Methods: Two hundred blood samples were collected from cattle with pale mucous membrane from regions with heavy tick endemicity. The samples were analyzed for the presence of Rickettsia pathogens using thin blood smears and the Diff-Quik stains. All the samples were also examined using polymerase chain reaction (PCR) to amplify the 16S ribosomal ribonucleic acid (rRNA) gene to confirm the presence of the smear-identified microorganisms. Ten PCR positive samples were subjected to 16S rRNA partial gene sequencing to identify the species. Results: The findings uncovered positivity in 68 (34%) blood smears. PCR revealed positive confirmation in 18 (9%) of the 200 blood samples. Mycoplasma wenyonii and Candidatus mycoplasma hemobos were identified from 10 PCR positive samples. The nucleotide sequences of the isolates were closely related to isolates from cattle, buffalo, and dogs in Vietnam, Cuba, India, and Germany. Conclusion: Bovine hemoplasma infections are prevalent in cattle in the Al-Qadisiyah Province in Iraq. Our results may have significance for the development of control programs.

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Prevalence and some risk factors of bovine heamotropic mycoplasma in Nineveh province - Iraq

Cited by 4 publications

References 14 publications

Seroprevalence of Crimean Congo Hemorrhagic Fever in cows by ELISA in Mosul city

Seroprevalence of Crimean Congo Hemorrhagic Fever in cows by ELISA in Mosul city

Prevalence of Bovine Viral Diarrhea Virus in Sheep in Nineveh Province

First study on microscopic and molecular evidences of two bovine hemoplasma species in cattle herds in Al-Qadisiyah Province, Iraq

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