Prevalence and temporal trends in antimicrobial resistance of bovine respiratory disease pathogen isolates submitted to the Wisconsin Veterinary Diagnostic Laboratory: 2008–2017

Abstract

The objective of this study was to describe the prevalence and trends in antimicrobial resistance for bacterial pathogens associated with bovine respiratory disease (BRD) isolated from samples submitted to the Wisconsin Veterinary Diagnostic Laboratory (WVDL). Data were retrospectively collected from bovine respiratory isolates including Pasteurella multocida, Mannheimia haemolytica, Histophilus somni, and Bibersteinia trehalosi identified at the WVDL between January 2008 and December 2017. Antimicrobial susceptibility testing data were queried from antimicrobial resistance databases at the WVDL. A total of 4,261 isolates were identified. Pasteurella multocida was most frequently identified, accounting for 2,094 isolates (49% of total) over the study period. Mannheimia haemolytica was the second most frequently isolated bacterial respiratory pathogen (n = 1,267, 30%) followed by H. somni (n = 749, 18%) and B. trehalosi (n = 151, 4%). Over the 10-yr period, B. trehalosi had the highest median percentage of isolates that were resistant to at least one antibiotic at 33% (interquartile range: 24, 47) followed by M. haemolytica (13%; 8, 23). For P. multocida, 10% (4, 26) of isolates were classified as resistant to at least one antibiotic, whereas H. somni had the fewest resistant isolates (9%; 3, 15). When comparing 2013-2017 to 2008-2012, the overall percentage of resistant isolates for P. multocida and B. trehalosi decreased, whereas the percentage of resistant isolates for M. haemolytica and H. somni increased. Increased resistance against florfenicol, fluoroquinolones, gentamicin, tilmicosin, and tulathromycin was observed for M. haemolytica. These data show that antimicrobial susceptibility for BRD bacterial pathogens has changed in the population served by the WVDL over this 10-yr period. For P. multocida, resistance is relatively low and has either improved or at least remained constant for the majority of drugs labeled for treatment of respiratory disease in dairy cattle. Veterinarians and producers should be aware of the bacterial pathogens most commonly associated with BRD and work toward early disease detection, proper antibiotic administration, and monitoring lung lesions to ensure that their treatment protocols improve lung health.

Antimicrobial Resistance in Isolates from Cattle with Bovine Respiratory Disease in Bavaria, Germany

“…With AMR of bacterial isolates on the rise, precise knowledge of the resistance situation at hand is essential for the targeted treatment of bacterial infections [ 29 , 30 , 32 , 34 ]. Since there is only an obligation to determine resistance in certain cases and antibiotic therapy must be started immediately in acute cases of the disease, the practicing veterinarian has to rely on existing data and studies on the local resistance situation [ 49 , 53 ].…”

“…Worldwide studies indicate that there is a trend towards increasing bacterial resistance towards certain antimicrobial agents, especially multidrug-resistance (MDR) when pathogens of BRD are investigated [ 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. In the context of the BRD complex, P. multocida and M. haemolytica isolates are categorized as MDR if they are not susceptible (resistant or intermediate) to at least one agent in at least three antimicrobial classes [ 38 ].…”

Whole-genome sequencing of Histophilus somni strains isolated in Russia

“…The problem of H. somni antibiotic resistance has been widely discussed in recent years [19][20][21]. In 2021, the phenotypic antibiotic resistance of the H. somni samples 188-VIEV, 532-VIEV, and 551-VIEV isolated in Russia was studied [22].…”

Antimicrobial resistance and associated genetic background of Histophilus somni isolated from clinically affected and healthy cattle

“…However, the excessive and unreasonable use of antimicrobials has accelerated selective pressure, which may affect the expression of antimicrobial resistance genes and enhance the emergence of resistant isolates in these microorganisms. Notably, an increase in the incidence of multidrug-resistant pathogenic bacteria has been reported in recent decades, and is gradually decreasing the efficacy of currently available antibiotics for BRD in North America ( 8 – 10 ). Regardless, there are only a few studies on the current AMR status of H. somni compared to other species of the family Pasteurellaceae ( 7 , 8 , 11 – 13 ).…”