Prevalence and types of class 1 integrons in aminoglycoside-resistant Enterobacteriaceae from several Chilean hospitals

Abstract

Members of the family Enterobacteriaceae are responsible for a variety of nosocomial infections, treatment of which is limited due to their increasing resistance to antibiotics. Some bacterial genes encoding antibiotic resistance comprise the major part of gene cassettes, most of which are associated with integrons. In this work, the carriage of class 1, 2 and 3 integrons was investigated in 191 Enterobacteriaceae isolates from clinical specimens of hospitalized patients. Class 1 integrons were found to be the most common, whereas no class 3 integrons were detected. The variable regions of 13 class 1 integrons were characterized and four types were found. Type 1 harbours only ant(3")I, type 2 harbours ant(2")I and ant(3")I, type 3 harbours aac(6')Ib and ant(3")I and type 4 lacks inserted gene cassettes.

A profile of drug resistance genes and integrons in E. coli causing surgical wound infections in the Faisalabad region of Pakistan

“…This highlights the increasing sophistication in the armory of these pathogens. The prevalence of integrons is relatively high as compared with some earlier reports, 10,11 yet is comparable with a report from the United States. 13 This high level of occurrence of integrons (62.6%) highlights the threat of MDR.…”

“…The simultaneous occurrence of two types of integrons in one E. coli isolate was significant, because it is relatively rare in clinical E. coli isolates. [10][11][12] To our knowledge, it is the first time in the world that two types of integrons have been amplified simultaneously in wound-infecting E. coli. This highlights the increasing sophistication in the armory of these pathogens.…”

Identification of Capsular Types in Carbapenem-Resistant Klebsiella pneumoniae Strains by wzc Sequencing and Implications for Capsule Depolymerase Treatment

“…Integrons are a type of mobile genetic elements thought to be associated with antibiotic resistance, and class 1 integrons were found to be the most common type of integrons in Enterobacteriaceae (45). Therefore, the presence of class 1 integrons in the 85 CRKP strains was investigated.…”

Characterization of Third-Generation-Cephalosporin-Resistant Shiga Toxin-Producing Strains of Escherichia coli O157:H7 in Japan

“…Plasmid genotypes were examined by the detection of integrons, replicon typing, and IncI1 plasmid multilocus sequence typing (IncI1 pMLST) as previously described (14)(15)(16). The PCR assay showed that all the conjugants contained a plasmid with the IncI1 replicon ( Table 1).…”