Prevalence, antimicrobial resistance and virulence genes of Salmonella serovars isolated from humans and animals

Dutta, Rupam; Das, Leena; Hazarika, G. C.; Choudhury, Mridusmita; Deka, Naba Kumar; Malakar, Dipika; Hussain, I.; Barkalita, Luit Moni

doi:10.1007/s11259-022-09900-z

Cited by 18 publications

(14 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…fepA was also present in a high percentage (64.7%) of isolates belonging to Salmonella serovars Enteritidis, Weltervreden, Typhi, Newport, Litchfield, Idikan and Typhimurium, as well as Salmonella SE3 and. Other virulence genes were present in varying percentages among the Salmonella serovars studied by Borah et al [ 66 ] such as sopB (86.36%), sopE2 (62.5%), pefA (79.54%) and sefC (51.14%); of these genes only sefC was not detected in Salmonella SE3. The virulence genes identified in Salmonella SE3 are involved in several different processes, such as the invA gene usually codes for a protein in the inner bacterial membrane that is responsible for the invasion of intestinal cells of the host [ 67 , 68 ].…”

Section: Discussionmentioning

confidence: 99%

“…Some of these virulence genes are also found in other serovars of Salmonella. Borah et al [ 66 ] investigated virulence genes in 88 Salmonella isolates recovered from humans and different species of animals. Among the 88 isolates, some virulence genes such invA , sipA , sipB and sipC were detected irrespective of the serovar, and these were also detected in Salmonella SE3.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Hybrid Genomic Analysis of Salmonella enterica Serovar Enteritidis SE3 Isolated from Polluted Soil in Brazil

et al. 2022

View full text Add to dashboard Cite

In Brazil, Salmonella enterica serovar Enteritidis is a significant health threat. Salmonella enterica serovar Enteritidis SE3 was isolated from soil at the Subaé River in Santo Amaro, Brazil, a region contaminated with heavy metals and organic waste. Illumina HiSeq and Oxford Nanopore Technologies MinION sequencing were used for de novo hybrid assembly of the Salmonella SE3 genome. This approach yielded 10 contigs with 99.98% identity with S. enterica serovar Enteritidis OLF-SE2-98984-6. Twelve Salmonella pathogenic islands, multiple virulence genes, multiple antimicrobial gene resistance genes, seven phage defense systems, seven prophages and a heavy metal resistance gene were encoded in the genome. Pangenome analysis of the S. enterica clade, including Salmonella SE3, revealed an open pangenome, with a core genome of 2137 genes. Our study showed the effectiveness of a hybrid sequence assembly approach for environmental Salmonella genome analysis using HiSeq and MinION data. This approach enabled the identification of key resistance and virulence genes, and these data are important to inform the control of Salmonella and heavy metal pollution in the Santo Amaro region of Brazil.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Hybrid Genomic Analysis of Salmonella enterica Serovar Enteritidis SE3 Isolated from Polluted Soil in Brazil

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Te sipB and invA genes were found in 100% of the isolates that were assessed (n � 49/49). Te sipB gene may play a vital role in Salmonella pathogenesis [44]. In the case where detection rates of the invA gene were expected, this gene is recognized as a rapid detection agent for the genus Salmonella, and this gene also indicates that all the strains are able to produce gastroenteritis and invade the cells [45,46].…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of Virulence Factors in Salmonella serovars Isolated from Poultry and Human Samples

Lozano-Villegas

Herrera‐Sánchez

Beltrán-Martínez

et al. 2023

Veterinary Medicine International

View full text Add to dashboard Cite

Salmonellosis is a common infectious disease in humans caused by Salmonella spp., which in recent years has shown an increase in its incidence, with products of avian origin being a common source of transmission. To present a successful infective cycle, there are molecular mechanisms such as virulence factors that provide characteristics that facilitate survival, colonization, and damage to the host. According to this, the study aims to characterize the virulence factors of Salmonella spp. strains isolated from broilers (n = 39) and humans (n = 10). The presence of 24 virulence genes was evaluated using end-point PCR. All the strains of Salmonella spp. isolated from broiler chickens revealed presence of 7/24 (29, 16%) virulence genes (lpfA, csgA, sitC, sipB, sopB, sopE, and sivH). Regarding the strains isolated from cases of gastroenteritis in humans, all strains contained (14/24, 58, 33%) virulence genes (lpfA, csgA, pagC, msgA, spiA, sitC, iroN, sipB, orgA, hilA, sopB, sifA, avrA, and sivH). In summary, the presence of virulence genes in different strains of Salmonella isolated from broilers and humans could be described as bacteria with potential pathogenicity due to the type and number of virulence genes detected. These findings are beneficial for the pathogenic monitoring of Salmonella in Colombia.

show abstract

“…The sefC gene was present in half of the isolated strains. The multitude of the virulence genes of Salmonella highlights the importance of continuous monitoring [ 28 ]. In addition, our results revealed that 33% (20/61) of Salmonella strains were multi-resistant to antimicrobials considered of great importance for human and animal health.…”

Section: Discussionmentioning

confidence: 99%

Serotype Occurrence, Virulence Profiles, Antimicrobial Resistance and Molecular Characterization of Salmonella Isolated from Hospitalized Patients with Gastroenteritis in Great Tunisia between 2010 and 2020

et al. 2023

View full text Add to dashboard Cite

Non-typhoid Salmonella is one of the major causes of food-borne infections worldwide. The aim of the current study is to determine the serotype occurrence, virulence factors and antimicrobial resistance patterns of Salmonella isolated from hospitalized patients. The identification of Salmonella strains was performed according to REMIC, 2018. The susceptibility of Salmonella isolates was assessed against 20 antimicrobials using the disk diffusion method. Some virulence and antimicrobial resistance genes were identified using PCR. Among the 61 isolated Salmonella strains, seven serotypes were identified and all were positive for the virulence genes invA, mgtC and sirA. Critical resistance rates (>40%) were detected for tetracycline, nalidixic acid, amoxicillin and fluoroquinolones. However, resistances to ertapenem, ceftazidim, aztreonam and colistin were null. In addition, 33% of the isolated strains were multidrug-resistant (MDR). Moreover, 80% and 60% of S. Kentucky isolates were identified as fluoroquinolone-resistant and MDR strains, respectively. The qnrB gene was amplified in 63.2% of fluoroquinolone-resistant strains. The dfrA1 gene was identified in 20% (4/20) of the trimethoprim-sulfamethoxazole resistant strains and the integrase Class 2 gene was amplified in only 8.2% (5/61) of the isolates. Our findings highlight the emergence of MDR Salmonella isolates. A rationalization of antimicrobial use is urgently recommended in both human and veterinary medicine.

show abstract

Prevalence, antimicrobial resistance and virulence genes of Salmonella serovars isolated from humans and animals

Cited by 18 publications

References 33 publications

Hybrid Genomic Analysis of Salmonella enterica Serovar Enteritidis SE3 Isolated from Polluted Soil in Brazil

Hybrid Genomic Analysis of Salmonella enterica Serovar Enteritidis SE3 Isolated from Polluted Soil in Brazil

Molecular Detection of Virulence Factors in Salmonella serovars Isolated from Poultry and Human Samples

Serotype Occurrence, Virulence Profiles, Antimicrobial Resistance and Molecular Characterization of Salmonella Isolated from Hospitalized Patients with Gastroenteritis in Great Tunisia between 2010 and 2020

Contact Info

Product

Resources

About