Prevalence, distribution and control of six major potato viruses in Kenya

Onditi, John; Nyongesa, Moses; Vlugt, R.A.A. van der

doi:10.1007/s40858-020-00409-x

Cited by 10 publications

(4 citation statements)

References 28 publications

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“…The high prevalence of PLRV, PVY, and other aphid-transmitted viruses in eld environments with potential aphid infestation is likely to accelerate the spread of aphid-borne viral infections, resulting in rapid and severe seed potato degeneration under bulking and low progeny crop yield [31]. When viral infections occur in combination, the degenerative effect is likely to be exacerbated [13], as observed in this study, where more than 80% of the samples from different districts with high potential to produce seed were infected by more than one virus at the same time. The synergism effect of most potato viruses occurring in multiple infections exacerbates potato crop economic and yield loss [9].…”

Section: Discussionmentioning

confidence: 99%

“…More than 50 viruses and one viroid have been identi ed as infecting potatoes around the world, causing varying degrees of loss depending on virus type, strain, seed system, and crop production practices [9]. Virus infection in potatoes is critical because it causes seed degeneration and a gradual but long-term decrease in yield, especially in agricultural systems where the use of certi ed seed is limited [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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Assessment of prevalence and distribution of potato viruses in Ethiopian seed systems

Tessema

Kakuhenzire

Seid

et al. 2023

Preprint

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Viruses are among the significant biotic constraints burdening global potato production, particularly in developing countries where seed certification is not common and production, availability and accessibility to quality seed potato is a big hindrance. Virus infection in seed potato reduces yield, and the problem is exacerbated when an early-generation seed is affected. The prevalence of six key potato viruses, PVY, PVX, PLRV, PVA, PVS, and PVM, was assessed among decentralized seed multipliers such as individuals, farmer seed group cooperatives, private companies, and agricultural research center that produce early generation seed in six major potato growing districts in Ethiopia. A total of 262 leaf samples were randomly collected from potato plants and analyzed using a double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for potato viruses. Potato virus prevalence was calculated as the proportion of samples that tested positive for the viruses against the total number of samples tested. Only 1.8% of the samples were virus-free, while 98.2% of the samples collected from farmers' fields were infected with at least one of the six viruses. About 17% of the samples had a single viral infection with one of the six viruses, whereas 81% had multiple infections. Early generation seed and germplasm samples collected from breeding, research, and private screenhouses were infected with PLRV (68.1%), PVY (57.4%), PVS (40.4%), PVM (34%) and PVX (23.4%). These results indicate that virus infection is widespread in the country, posing challenges and threats potato production and food security efforts. To address this issue, it is critical to developing a robust system that prevents viral infection build-up and spread in the seed system through regular seed quality assurance and certification, particularly for early generation seed. Furthermore, smallholder farmers need to use an integrated management approach to effectively combat potato viruses.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of prevalence and distribution of potato viruses in Ethiopian seed systems

Tessema

Kakuhenzire

Seid

et al. 2023

Preprint

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“…The mean separation analysis of extracted data was performed using LSD test function in the agricolae package with alpha = 0.05 in in R. Studio .4.4 (De Mendiburu, 2014). Spider diagrams were used for decision making of effective begomoviruses control (Onditi et al, 2021). Decision making involves to set overall goal and set criteria to achieve that overall goal for effective Begomoviruses control in the Pakistan farming systems (Florin et al, 2014).…”

Section: Sampling and Data Collectionmentioning

confidence: 99%

Farmers’ Perception and Knowledge in Begomovirus Epidemiology and Control in Pakistan

Bahar

Rauf

Muqeet³

et al. 2023

Int. J. Phytopathol.

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Agriculture is the backbone of Pakistan and the growing crops are called as the “spinal cord” of the state but still import bill of the country agricultural products is rising day by day and issues of the food security is also becoming grave because of less knowledge of farmers about the emerging abiotic and biotic stresses affecting the crop productivity. Among various biotic stresses, prevalence of begomoviruses is considered to be a major constraint in reducing the yields of economically important food and fiber crops. Farmers’ perception and their knowledge regarding begomoviruses epidemiology and practices are the pre-requisites for effective virus control. A farmer survey mostly having less than 12.5 acres land mostly of vegetables, rice and maize growers involving 250 randomly selected respondents from five major districts (Nankana, Sheikhupura, Gujranwala, Gujrat, and Lahore) of Punjab, Pakistan to inquire from them whether they are familiar with begomoviruses identification, their means of transfer from diseases inoculum to healthy plants and associated losses in fields, proper management of these losses on time, was conducted to better understand the farmer knowledge and practices and to set the possible pathways for intervention of effective virus control. Lack of sufficient technical information regarding vector of begomovirus, their transmission and disease symptoms was the major hindrance to the efforts of farmers for effective virus control. In addition, the farmers did not have sufficient knowledge of locally available methods of begomovirus control. The highest prioritization aspect of virus control for immediate attention were determined by decision making such as spider diagram. Use of effective pesticides, certified planting material, and begomovirus tolerant cultivars were found to be the most attention requiring virus control aspects which could have a greater influence to lower the virus prevalence in field crops. Present study suggests that enhancing the farmer knowledge is a highest prioritized key determinant of effective virus control strategy for implementation in Pakistan.

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“…Commercially important potato viruses cause a reduction in the starch content of tubers [11] and other biochemical and physiological changes manifested by a reduction in dry matter and vitamin content, starch granules, a decrease in amylase content, and starch acidity [11,12]. In addition to poor vegetative growth (stunting, dwarfing) and significant yield losses in tubers, viruses in potato plants can cause symptoms such as leaf curling, mosaic, leaf drop, chlorosis, necrosis, reduced product quality, undersized tubers, and sometimes death of the entire plant [11][12][13][14][15]. Potato leafroll virus (PLRV), potato virus A, M, S, X, and Y (PVA, PVM, PVS, PVX, and PVY) are usually the most important viral pathogens associated with significant economic impact and production loss [9,15,16].…”

Section: Introductionmentioning

confidence: 99%

Partial Elimination of Viruses from Traditional Potato Cultivar ‘Brinjak’ by Chemotherapy and Its Impact on Physiology and Yield Components

Kereša

Vončina²,

Lazarević³

et al. 2022

Horticulturae

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Viruses are responsible for more than 50% of annual potato tuber yield losses and cause great economic damage. The traditional Croatian potato cultivar ‘Brinjak’ is important for local growers because of its economically profitable production and as a gene pool for future breeding programs. However, the full genetic potential of the cultivar cannot be exploited due to virus infection. In this study, we attempted to eliminate potato virus M (PVM) and potato virus S (PVS) from potato cultivar ‘Brinjak’ and to evaluate the effects on physiological parameters and yield. Shoot apices were isolated from PVM + PVS-infected sprouts and cultivated for six weeks on MS medium with the addition of 50 or 100 mg L−1 ribavirin. The surviving shoot apices were micropropagated. The in vitro post-eradication period lasted 200 days. DAS-ELISA and RT-PCR were performed on R0 and R1 plants 90 days after acclimatization to determine the sanitary status of the plants. Chlorophyll fluorescence and multispectral imaging were performed on the R0 plants at the same time. The success of PVS elimination was 33% at both ribavirin concentrations. However, neither concentration was successful in eliminating PVM. Plants with mixed infection (PVM + PVS) had more severe disease symptoms compared to PVM-infected plants, affecting photochemistry and multispectral parameters and, consequently, yield. PVM + PVS plants had significantly lower number and weight of tubers per plant and lower average tuber weight than plants with single PVM infection in most of the generations studied. The results indicate a strong negative impact of PVS in mixed infections with PVM and show the importance of its elimination from potato plants.

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Prevalence, distribution and control of six major potato viruses in Kenya

Cited by 10 publications

References 28 publications

Assessment of prevalence and distribution of potato viruses in Ethiopian seed systems

Assessment of prevalence and distribution of potato viruses in Ethiopian seed systems

Farmers’ Perception and Knowledge in Begomovirus Epidemiology and Control in Pakistan

Partial Elimination of Viruses from Traditional Potato Cultivar ‘Brinjak’ by Chemotherapy and Its Impact on Physiology and Yield Components

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