Prevalence of 16S rRNA methylase genes among β-lactamase-producing Enterobacteriaceae clinical isolates in Saudi Arabia

Sheikh, Yazeed A. Al; Marie, Mohammed Ali M.; John, James; Krishnappa, Lakshmana Gowda; Dabwab, Khaled Homoud M.

doi:10.3402/ljm.v9.24432

Cited by 27 publications

(23 citation statements)

References 29 publications

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“…In Saudi Arabia, the relationship between ESBL and 16S rRNA methyltransferase genes was investigated, and the rmtB , rmtC , and armA genes were detected in the same isolates (24). Fritsche et al analyzed gram-negative bacteria isolates from North America, Latin America, and Europe and found that the armA , rmtB , and rmtD genes were resistant to aminoglycosides in isolates with amikacin MIC values of over 128 µg/L (25).…”

Section: Discussionmentioning

confidence: 99%

NDM-1 and rmtC-Producing Klebsiella pneumoniae Isolates in Turkey

Gökmen

Nağıyev

Meral

et al. 2016

Jundishapur J Microbiol

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BackgroundThe resistance of aminoglycosides in strains that produce beta-lactamase can be developed through the multidrug resistant encoding genes carried by common plasmids. Recently, the association between 16S rRNA methyltransferase resistance and beta-lactamase enzymes carried by the same plasmids has drawn increased attention from researchers, particularly the association in aminoglycoside-resistant strains with a minimum inhibitory concentration (MIC) of ≥ 256 µg/mL.ObjectivesWe aimed to investigate the co-existence of 16S rRNA methyltransferase and beta-lactamase genes in multidrug resistant (MDR) Klebsiella pneumoniae strains isolated from clinical samples.MethodsWe determined the molecular mechanisms of aminoglycoside resistance and its relationship with resistance to carbapenem and beta-lactam group antibiotics in 40 extended-spectrum beta-lactamase (ESBL)-positive carbapenem- and aminoglycoside-resistant K. pneumoniae strains. Multidrug resistant K. pneumoniae was isolated from various clinical samples in the faculty of medicine of Cukurova University, Turkey. First, the resistance of aminoglycoside and beta-lactam antibiotics was phenotypically investigated using the Kirby-Bauer disk diffusion test, double disk synergy test, and modified Hodge test. The MIC values of aminoglycoside were determined using the agar dilution method. Polymerase chain reaction was performed to detect the carbapenemases, ESBL, and 16S rRNA methyltransferase genes. The results were confirmed by a sequence analysis.ResultsTwenty K. pneumoniae strains showed resistance to amikacin, and 40 were resistant to gentamicin. The MIC value was found to be > 512 µg/mL in five amikacin-resistant strains and > 128 µg/mL in 10 gentamicin-resistant isolates. The rmtC gene, a type of 16S rRNA methyltransferase, was amplified in four isolates (MIC amikacin: > 512 µg/mL, gentamicin: > 128 µg/mL). Of these four isolates, three had the blaNDM-1 gene and all contained at least one ESBL gene.ConclusionsThis study demonstrated the co-existence of rmtC and blaNDM-1 genes for the first time in Turkey. The spread of this resistant type should be monitored and limited through molecular surveillance.

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Section: Discussionmentioning

confidence: 99%

NDM-1 and rmtC-Producing Klebsiella pneumoniae Isolates in Turkey

Gökmen

Nağıyev

Meral

et al. 2016

Jundishapur J Microbiol

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“…Different genes reported for conferring antimicrobial resistance to various drugs were targeted: for beta-lactams (carbapenems, cephalosporins, penicillins) (bla TEM-1 , (Firoozeh et al 2012) and for efflux pumps (qnrA, qnrB, qnrS, qnrC, QnrD, AAC(6 0 )-Ib-cr, armA, rmtA, rmtB, rmtC, rmtD, npmA, qepA, qacE-sul1) (Whichard et al 2007;Ma et al 2009;Al Sheikh et al 2014;Pribul et al 2016). The primer sequences and annealing temperatures are described in Table 1.…”

Section: Detection Of Drug Resistance Genesmentioning

confidence: 99%

Detection and characterization of multidrug‐resistant Salmonella enterica serovar Infantis as an emerging threat in poultry farms of Faisalabad, Pakistan

et al. 2019

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Aims The aim of this study was the molecular identification of Salmonella enterica serovar Infantis (S. Infantis) isolated from poultry samples and their antimicrobial resistance and virulence profiling. Methods and Results A total of 149 isolates, belonging to genus Salmonella, originally isolated from 340 suspected poultry post mortem specimens reported by us earlier were preliminary identified as Salmonella by biochemical methods and confirmed by polymerase chain reaction targeting genus‐specific gene invA. Targeting serovar‐specific gene fragment (fljB) resulted in confirmation of 54 isolates as S. Infantis which were further confirmed by sequencing of 16S RNA and fljB genes. Swimming and swarming motilities were detected in 98·1 and 11·1% isolates respectively. Phenotypic disc diffusion assay against 23 antimicrobial agents showed the highest resistance against pefloxacin (PEF) (94·4%), chloramphenicol (83·3%) and imipenem (77·7%) while 5·3% isolates showed extended‐spectrum β‐lactamase production. Fifty‐nine genes reported for antimicrobial resistance and 12 for conferring virulence were targeted. The most prevalent resistance gene for aminoglycosides was aadA (42·3%), for quinolone resistance determining region parE (62·5%), for penicillin's Int1 (62·9%), for chloramphenicol cat3 (66·1%) and for beta‐lactams blaTEM‐1 (44·4%). Among efflux pump coding genes, armA showed highest (74·2%) prevalence and for virulence, a high prevalence of SopE (89·2%) showed the zoonotic potential of the isolates. The activity of efflux pumps was detected through Ethidium Bromide‐agar method. Conclusions Poultry could act as reservoirs of multidrug resistance Salmonella. Significance and Impact of the Study We firstly report the prevalence and molecular characterization of virulence/drug resistance in S. Infantis from this region and the results may contribute to designing precisely targeted therapy. This study has also highlighted the possible emergence of S. Infantis with zoonotic potential.

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“…47 Of 330 unique Enterobacteriaceae clinical isolates collected at a hospital in Saudi Arabia in 2011, a total of 37% carried at least one 16S-RMTase gene. 35 …”

Section: Prevalence Of 16s Ribosomal Rna Methyltransferasesmentioning

confidence: 99%