Prevalence of adhesive genes among uropathogenic Escherichia coli strains isolated from patients with urinary tract infection in Mangalore

Av, Shetty; Kumar, Sudhir; Shekar, Malathi; Shetty, Anil; Karunasagar, Iddya

doi:10.4103/0255-0857.129812

Cited by 20 publications

(19 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The higher number of samples having pap and sfa genes together as compared with other combinations could be due to the localization of these genes on the same pathogenicity island of UPEC strains [36]. In our study, the combination of pap/sfa genes was more frequently detected than pap/afa genes, which is similar to previous studies [36,37]. Cellular morphology and molecular biology studies have revealed that UPEC express siderophore production peculiar to the strains of E. coli causing extraintestinal infections [38].…”

Section: Discussionsupporting

confidence: 88%

“…In another study by Usein et al, the prevalence rates of fimH, sfa/foc, papC, and afa in Romanian adults with UTI was 86%, 23%, 36%, and 14%, respectively [35]. The higher number of samples having pap and sfa genes together as compared with other combinations could be due to the localization of these genes on the same pathogenicity island of UPEC strains [36]. In our study, the combination of pap/sfa genes was more frequently detected than pap/afa genes, which is similar to previous studies [36,37].…”

Section: Discussionmentioning

confidence: 94%

See 1 more Smart Citation

Detection of virulence genes, phylogenetic group and antibiotic resistance of uropathogenic Escherichia coli in Mongolia

Yandag

Gunregjav

Altantsetseg

et al. 2017

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: The severity of urinary tract infection (UTI) produced by uropathogenic Escherichia coli (UPEC) is due to the expression of a wide spectrum of virulence genes. E. coli strains were divided into four phylogenetic groups (A, B1, B2 and D) based on their virulence genes. The present study aimed to assess the relationship between virulence genes, phylogenetic groups, and antibiotic resistance of UPEC. Methodology: A total of 148 E. coli were tested for antimicrobial resistance against 10 drugs using the disk diffusion method. The isolates were screened by polymerase chain reaction (PCR) for detection of virulence genes and categorized into the four major phylogenetic groups. Results: Phylogenetic group B2 was predominant (33.8%), followed by D (28.4%), A (19.6), and B1 (18.2%). A higher prevalence of fimH (89.9%), fyuA (70.3%), traT (66.2%), iutA (62.2%), kpsMTII (58.8%), and aer (56.1%) genes were found in UPEC, indicating a putative role of adhesins, iron acquisition systems, and protectins that are main cause of UTIs. The most common antibiotic resistance was to cephalotin (85.1%), ampicillin (78.4%) and the least to nitrofurantoin (5.4%) and imipenem (2%). In total, 93.9% of isolates were multidrug resistant (MDR). Conclusions: This study showed that group B2 and D were the predominant phylogenetic groups and virulence-associated genes were mostly distributed in these groups. The virulence genes encoding components of adhesins, iron acquisition systems, and protectins were highly prevalent among antibiotic-resistant UPEC. Although the majority of strains are MDR, nitrofurantoin is the drug of choice for treatment of UTI patients in Ulaanbaatar.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 94%

Detection of virulence genes, phylogenetic group and antibiotic resistance of uropathogenic Escherichia coli in Mongolia

Yandag

Gunregjav

Altantsetseg

et al. 2017

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…The sfa gene was found in twelve (70.6 %) of the isolates together with pap genes. Shetty et al (2014) explained that co-existence of these two genes are due to their localization on the same pathogenicity island of UPEC strains 30 . In addition, most of the isolates carried multiple adhesion genes, indicating A and D groups in each population can vary according to various factors (geographic/climatic conditions, dietary factors, the use of antibiotics, host genetic factors) and commensal strains can acquire virulence factors and become potentially pathogenic 37 .…”

Section: Determination Of Antimicrobial Resistance and Virulencementioning

confidence: 99%

Phylogenetic Group/Subgroups Distributions, Virulence Factors, and Antimicrobial Susceptibility of Escherichia coli Strains from Urinary Tract Infections in Hatay

Yılmaz

Aslantaş

2020

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: Nosocomial and community acquired urinary tract infections (UTIs) are one of the most encountered infections in the world. Methods: This study aimed to determine the antibiotic susceptibility, phylogeny, and virulence genes of 153 Escherichia coli strains isolated from UTIs. Antimicrobial susceptibility of the isolates to different classes of antimicrobials was determined by the VITEK-2 automated system. Presence of virulence genes and phylogenetic groups were investigated by PCR. Results: Regarding susceptibility to antimicrobials, ampicillin resistance was most abundant (67.3%), followed by amoxicillin-clavulanic acid (50.9%); least abundant was resistance to amikacin (1.3%) and nitrofurantoin (1.3%). Multi drug resistance (MDR) was observed in 34.6% of the isolates, and all isolates were found to be susceptible to imipenem, meropenem and fosfomycine. The majority of the isolates belonged to the phylogenetic group B2 3 (35.9%), followed by A1 (20.9%), D1 (18.9%), D2 (12.4%), A0 (%5.9), B1 (3.9%) and B2 (1.9%). Among E. coli strains examined, 49% had iucD, 32.7% papE-F, 26.1% papC, 15% cnf2, 11.1% sfa, 7.8% cnf1, 1.3% afaE, 1.3% afaD, 1.3% hlyA, 0.7% f17a-A, 0.7% clpG and 0.7% eaeA genes. Conclusions: Our research demonstrated that virulence factors were distributed among different phylogroup/subgroups, which play a role in UTIs pathogenesis in humans. For this reason, complex and detailed studies are required to determine the relationship between virulence factors and specific E. coli strains that cause UTIs in humans. FIGURE 1: PCR amplification of E. coli specific 16S rRNA gene (401 bp).

show abstract

“…In the present study, we found very high frequency of the sfa gene in the hospitalized RUTIs patients, where all the isolates were positive in PCR amplification. Previous studies have concluded that more than 50% of the sfa1 + isolates of E. coli in the UTIs have a high prevalence of virulence factors and pose a serious threat to the health of the patients (28). Adhesion and fimbrial virulence factors in E. coli such as sfa, afa, fim, etc., cause further risks in the development of RUTIs, especially in women (29).…”

Section: Discussionmentioning

confidence: 99%

The characterization of multidrug resistant type 1 S-fimbriated <i>Escherichia coli </i>from women with recurrent urinary tract infections (RUTIS) in Bangladesh

Hasan¹,

Momtaz²,

Foysal³

et al. 2018

Af J Clin Exp Micro

View full text Add to dashboard Cite

The characterization of multidrug resistant type 1 S-fimbriated Escherichia coli from women with recurrent urinary tract infections (RUTIs) in Bangladesh ABSTRACT Background: Escherichia coli is a bacterial species that is most frequently associated with urinary tract infections (UTIs) worldwide. Recurrent UTIs (RUTIs) pose a major threat to health, especially in women. However, studies on the pathogenic potentials of E. coli isolates associated with RUTIs has yet to be done. The present study was designed to characterize the isolates of E. coli from women, suffering from repeated UTIs in Bangladesh. Methods: A total of 15 isolates of E. coli, collected from women with RUTIs in the Sylhet city of Bangladesh, were analyzed by a series of biochemical and molecular tests. Results: The PCR amplification of the mannose-resistant type 1 S-fimbriae gene (sfa1) confirmed all sfa1 + isolates of E. coli. In an antibiogram assay, all the isolates of E. coli were found to be completely resistant to at least five of the ten commercial antibiotics and drug resistance were found to be correlated with the weight of the RUTIs patient. Analysis of the 16S rRNA data of the two most drug-resistant and clinically significant isolates were found to be clustered with the drug-resistant UTI-causing E. coli isolates, circulated in India. Conclusions: This study could pave the way of RUTIs diagnosis and treatment strategy for women in Bangladesh.

show abstract

Prevalence of adhesive genes among uropathogenic Escherichia coli strains isolated from patients with urinary tract infection in Mangalore

Cited by 20 publications

References 14 publications

Detection of virulence genes, phylogenetic group and antibiotic resistance of uropathogenic Escherichia coli in Mongolia

Detection of virulence genes, phylogenetic group and antibiotic resistance of uropathogenic Escherichia coli in Mongolia

Phylogenetic Group/Subgroups Distributions, Virulence Factors, and Antimicrobial Susceptibility of Escherichia coli Strains from Urinary Tract Infections in Hatay

The characterization of multidrug resistant type 1 S-fimbriated <i>Escherichia coli </i>from women with recurrent urinary tract infections (RUTIS) in Bangladesh

Contact Info

Product

Resources

About