Prevalence of Aeromonas spp and their virulence factors isolated from frozen chicken meat products

Abstract

A total of hundred samples of frozen chicken products represented by breast, thigh, nuggets and burger (25 of each) were randomly collected to study the prevalence of Aeromonas spp and their virulence factors in the examined products. the study showed that the mean values of psychrotrophic count were 8.17×10 3 ± 1.42×10 3 , 1.95×10 4 ± 2.06×10 4, 3.63×10 4 ± 0.89×10 4 and 7.58×10 4 ± 1.16×10 4 , respectively, and the mean values of Aeromonas counts were 9.34×10 2 ± 2.01×10 2 , 1.66×10 3 ± 0.28×10 3 , 2.90×10 3 ± 0.43×10 3 and 5.25×10 3 ± 0.69×10 3 for examined frozen breast, thigh, nuggets and burger. 12 isolates of A. hydrophila were specific for 16S rRNA gene of which 9 isolates were positive for aerolysin (aerA) and 10 of isolates were positive for haemolysin (ahhl), with incidence of 75% and 83.3%, respectively. The results achieved in the current study showed contamination of chicken products by Aeromonas spp. It is necessary to give more consideration to Aeromonads because they have the ability of toxins production, survival under low temperatures and growing in a wide spectrum of environments. So, hygienic measures should be adopted to control microbial contamination.

Characterization of Escherichia coli and other bacteria isolated from condemned broilers at a Danish abattoir

“…mirabilis (18.6%), and S . aureus (8.5%); findings that corroborate previous studies ( Kitai et al, 2005 ; Brahmabhatt, 2011 ; Beninati et al, 2015 ; Owuna et al, 2015 ; Skočková et al, 2015 ; Bortolaia et al, 2016 ; Davis et al, 2018 ; Li et al, 2019 ; Sanches et al, 2019 , 2021 ; Sarker et al, 2020 ; Sheir et al, 2020 ; Yu et al, 2021 ). During slaughter, carcasses are likely contaminated at various stages of processing by the microbial flora from the digestive tract, skin, and feathers, as well as by bacteria present on surfaces on processing equipment and in the chillers ( Rouger et al, 2017 ).…”

Microbiological Assessment of Various Ready-To-Eat Foods in Cairo, Egypt, and Studying the Possible Antibacterial Effects of Garlic and Cumin Oils as Food Additives

“…In the current study, the results illustrate that Aeromonas species were isolated from barbecued chicken meat (11.1%) and grilled fish (27.3%). The results of previous studies [2,39] coincided with ours as Aeromonas species were isolated from ground chicken meat. Other studies reported higher levels of contamination by Aeromonas species (47.17% and 57%) in chicken meat samples, respectively [40,41].…”

“…However, RTE foods can be involved in probable health risks due to their potential microbial contamination with foodborne pathogens at any stage of food chain preparation [1]. When RTE foods are handled in unsanitary circumstances where pathogenic bacteria exist in the equipment, workers' aprons/hands, and surrounding environment, the final RTE food end product could be contaminated [2]. The major foodborne pathogens that could infect humans through ingestion of contaminated food are, for example, Staphylococcus aureus, Salmonella spp., Vibrio cholera, Streptococcus spp., Klebsiella pneumoniae, Micrococcus spp., Clostridium botulinum, Aeromonas hydrophila, Escherichia coli, Bacillus spp., Enterobacter cloacae, Egypt Acinetobacter, Proteus mirabilis, Pseudomonas species, Citrobacter freundii, and Serratia species.…”

Green nanotechnology for controlling bacterial load and heavy metal accumulation in Nile tilapia fish using biological selenium nanoparticles biosynthesized by Bacillus subtilis AS12

“…The bacterial count was expressed as log CFU ml −1 for water and fish samples. The total bacterial count was enumerated on a plate count agar (PCA) previously supplemented with 100 μl of each dilution (fish and water samples), then incubated at 37°C for 1 day (Saad et al, 2021), while the Aeromonas count was calculated on an Aeromonas agar medium after 1 day of incubation at 37°C (Sheir et al, 2020). The Aeromonas spp., colonies were distinguished by dark green with blackcentered colonies.…”