Prevalence of Anaplasma marginale in cattle blood samples collected from two important livestock regions in Punjab (Pakistan) with a note on epidemiology and phylogeny of parasite

Zafar, Saba; Khan, Adil; Niaz, Sadaf; Aktaş, Münir; Özübek, Sezayi; Farooq, Muhammad; Adil, Muhammad Moeen; Zając, Zbigniew; Alhimaidi, Ahmad R.; Swelum, Ayman A.

doi:10.1016/j.sjbs.2021.11.020

Cited by 20 publications

(29 citation statements)

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“…The calves showed higher infection rate of 30.7% followed by adults at 16.4% and heifers 6.2%. It is in agreement with study of Zafar et al (2022) who reported prevalence of A. marginale from 9-17% in tested blood samples from two southern districts (Lodhran and Dera Ghazi Khan) of Punjab, Pakistan. Our findings are also in agreement with results of Atif et al (2022) who reported the highest prevalence of A. marginale in cattle of less than 1 year old (32.84%) while the lowest prevalence (6.45%) was in animals aged between 1 and 2 years of age.…”

Section: Discussionsupporting

confidence: 93%

Occurrence of ticks and tick-borne mixed parasitic microbiota in cross-bred cattle in District Lahore, Pakistan

et al. 2022

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The present study was focused on the incidence of ticks and tick-borne diseases (TTBD) in cross-bred cattle (Friesian x Sahiwal) of two farms (n = 2548) in district Lahore, Pakistan. We collected total of 572 ticks (adults and nymphs) and blood samples (10 ml) for microscopic i.e., blood smear test - Giemsa Stain (BST) and molecular analysis; Reverse Line Blot-General Primer-PCR (RLB-PCR) and Specie Specific Primer PCR (SP-PCR) from infested cattle (n = 100) from months of April to September. Results: The tick specie identified was Rhipicephalus microplus at both farms, with significant difference in infestations rate amongst both farms (p< 0.0001). The cross-bred cattle having higher ratio of Friesian blood and lower ratio of Sahiwal blood were mostly infested by ticks (p < 0.0458) and haemoparasites (p <0.474) and vice versa. The SP-PCR showed higher number of haemoparasites infection than BST, which revealed 16% T. annulata (p < 0.0001 and k value 0.485, 0.0001), 51% B. bigemina (p < 0.0001 and k value 0.485, 0.0001) and 15% A. marginale (p < 0.001 and k value 0.207, 0.001), respectively. The single infection with B. bigemina was 34% (n = 34/100) and A. marginale 6% (n = 6/100). The double infection with T. annulata/B. bigemina was 8% (n = 8/100) and B. bigemina/A. marginale 1% (n = 1/100). Whereas the triple infection with T. annulata/B. bigemina/A .marginale was 8% (n = 8/100). The phylogenetic study of isolated sequence of T. annulata revealed close homology to isolates from Iran (87%), B. bigemina to isolates from Cuba (94 to 100%) and A. marginale with isolates from Pakistan (99 to 98%).

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Section: Discussionsupporting

confidence: 93%

Occurrence of ticks and tick-borne mixed parasitic microbiota in cross-bred cattle in District Lahore, Pakistan

et al. 2022

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“…In Southern Punjab, Jhang, and KPK, the prevalence of mix infection was higher in tick-infested animals as compared to non-tick-infested animals (Ashraf et al, 2013;Atif et al, 2021). Our results are also in line with the results done in Lodhran and Dera Ghazi Khan where TBPs were more prevalent in tick-infested animals than the animals with no tick infestation history (Zafar et al, 2022). Similar findings have also been reported by Farooqi et al (2018), Parveen et al (2021), andInci et al (2008).…”

Section: O N L I N E F I R S T a R T I C L Esupporting

confidence: 91%

Analysis of the Development of Snakehead (Channa argus) Industry in Shandong, China and Comparison with the Substitution of Forage Fish with Compound Feed

Li¹

2023

PJZ

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“…Recently, Asif et al [1] has reported that 11% cattle located in Multan District of Punjab province in Pakistan were A. marginale infected. In a similar study, Zafar et al [5] has reported 9% and 11% prevalence of A, marginale in cattle from Lohdran and Dera Ghazi Khan Districts in Punjab respectively. Ashraf et al [9] and Hussain et al [44] has reported 8.6% and 6.1% infection rate of A. marginale in cattle blood samples collected from Layyah and Bahawalpur Districts in Punjab respectively.…”

Section: Plos Onementioning

confidence: 72%

“…with exotic cattle has made Pakistan endemic for tick-borne diseases [5,16]. Bajaur is a mountainous district in KPK province of Pakistan the population of whom is directly or indirectly dependent on livestock sector for their earning and survival [12] but the prevalence of TBD S in general and bovine theileriosis and anaplasmosis in specific has not been investigated.…”

Section: Plos Onementioning

confidence: 99%

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Simultaneous molecular detection of Anaplasma marginale and Theileria annulata in cattle blood samples collected from Pakistan-Afghanistan boarder region

Jamil,

Chiou,

Muqaddas

et al. 2023

PLoS ONE

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Theileria annulata (T. annulata) and Anaplasma marginale (A. marginale) are among the most extensively reported tick borne pathogens and are associated with huge economic losses worldwide. A total of 298 cattle blood samples were screened to report the presence of these two pathogens. The samples were collected from apparently healthy cattle (Achai, n = 155, Jersy, n = 88 and crossbred, n = 55) in Bajaur district of Khyber Pakhtunkhwa (KPK) during June and July of 2022. A total of 31 out of 298 cattle (10.4%) were found infected with T. annulata as PCR amplified a 156 base pair fragment from Tams-1 gene of T. annulata from their blood. While 16/298 animals (5.4%) were found infected with A. marginale as they amplified a 382 base pair fragment specific for msp5 gene of this bacterium. Three animals (1%) were found co infected. Cattle susceptibility to T. annulata infection was significantly higher than A. marginale infection (P < 0.001). Phylogenetic analysis revealed that Pakistani isolates of both detected pathogen clustered together and were closely related isolates from worldwide countries. Prevalence of T. annulata varied significantly among the sampling sites (P = 0.05) while no such association was observed for A. marginale among the tested cattle. Epidemiological data analysis revealed that none of the studied risk factors was found associated either with the prevalence of T. annulata or A. marginale (P > 0.05) among enrolled cattle. In conclusion, our study has revealed a relatively higher prevalence of T. annulata than A. marginale in cattle from the Bajaur district in KPK. This information is important for improving the productivity of the livestock sector, which is one of the main sources of income in the country. It is recommended that this data be taken into account for the development and implementation of effective tick control programs, as well as for the improvement of livestock management practices to prevent and manage TBDs in Pakistan.

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Prevalence of Anaplasma marginale in cattle blood samples collected from two important livestock regions in Punjab (Pakistan) with a note on epidemiology and phylogeny of parasite

Cited by 20 publications

References 19 publications

Occurrence of ticks and tick-borne mixed parasitic microbiota in cross-bred cattle in District Lahore, Pakistan

Occurrence of ticks and tick-borne mixed parasitic microbiota in cross-bred cattle in District Lahore, Pakistan

Analysis of the Development of Snakehead (Channa argus) Industry in Shandong, China and Comparison with the Substitution of Forage Fish with Compound Feed

Simultaneous molecular detection of Anaplasma marginale and Theileria annulata in cattle blood samples collected from Pakistan-Afghanistan boarder region

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