Prevalence of and Risk Factors for Listeria Species on Dairy Farms

Abstract

This cross-sectional study determined the prevalence of Listeria spp. in bulk-tank milk on dairy farms in the region of Galicia in northwest Spain. The aim was to identify management practices associated with the presence of Listeria spp. and possible effects on milk hygienic quality. A total of 98 farms was randomly selected on the basis of an expected prevalence of 6.5% for Listeria monocytogenes from 20,107 dairy farms in the region. Bulk-tank milk samples were obtained from 98 farms, fecal samples from lactating cows from 97 farms, and silage samples from 83 farms. Listeria monocytogenes was detected in 6.1, 9.3, and 6.0% of these samples, respectively. Statistical analyses confirmed the relationship between low silage quality (as indicated by high pH) and presence of Listeria spp. in silage (29.5 vs. 6.2% for pH above or below 4.5, respectively). Only milking system [tie-stall systems (28.6%) vs. parlor milking (10%)] and inadequately controlled milking order [yes (32.0%) vs. no (10.7%)] had statistically significant effects on management practices for increasing the risk of Listeria contamination of bulk-tank milk.

Isolation and determination of antimicrobial resistance of Arcobacter species isolated from animal faeces in the Diyarbakir region of Turkey using the 16S rDNA-RFLP method

“…Kabeya et al (2003) reported the Arcobacter spp. prevalence to be 3.6% in Japan, while Aydin et al (2007) reported it to be 6.9% in Turkey, Van Driessche et al (2003) found a prevalence of 39% in Belgium and Vilar et al (2010) found one of 41.7% in Galicia. Kabeya et al (2003) isolated A. butzleri, A. cryaerophilus andA.…”

Türkiye’nin Güneydoğu Bölgesi’nde Üretilen Mısır Silajı ve Çiğ Sütlerde Listeria Türlerinin Varlığı

“…Sağun et al [17] determined that 2.4% of raw milk samples obtained in Van province located in eastern Turkey, were positive for Listeria spp., where L. monocytogenes was found in 1.2%, L. innocua and L. welshimeri in 0.4% of those samples. Vilar et al [11] detected Listeria spp. in 16.3% of bulk-tank milk samples, where L. monocytogenes was found in 6.1%, L. innocua in 7.1%, L. welshimeri in 1.0% and L. grayi in 2.0% of those samples.…”

“…In the same study [10] , L. monocytogenes was isolated from 2.3% of corn samples and 2.6% of hay silage samples, which are similar with our results. Vilar et al [11] [12] isolated L. monocytogenes in 6.66% of silage samples. These results on L. monocytogenes in silage samples are higher than the result obtained from our study.…”

“…in silage samples was examined by several studies [10][11][12]14] . In a study, Listeria were isolated from 10% of corn silage samples, 28% of hay silage samples, and 60% of grass silage samples [10] .…”

“…It has been reported that listeriosis in cattle is mainly feed-borne and Listeria spp. have been detected in a ratio of between 1.2% and 60% from the silage samples [9][10][11] . Furthermore, Taşçı et al [12] reported that 6.6% of silage samples and 1.17% of milk samples obtained from cows fed with silage were contaminated with L. monocytogenes.…”