Prevalence of antibodies against<i>Entamoeba histolytica</i>in Mexico measured by ELISA

Gonzalez, Christian; Isibasí, Armando; Ortiz-Navarrete, Vianney; Paniagua, Jorge; García, José; Ramírez, A. D. de; Salvatierra, Benito; Tapia, Ricardo; Sepúlveda, Jorge; Gutiérrez, Gonzalo; Kumate, J

doi:10.1017/s0950268800058702

Cited by 14 publications

(13 citation statements)

References 9 publications

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“…Work has been done on the prevalence of E. histolytica with respect to gender. Similar findings of more prevalence was recorded in females (1.25%) than males (1%) in Mexico (Das et al, 2006) and (29.4%) in females (19.4%) in males in Brazil (Gonzalez et al, 1995). Similar findings were also reported in Iran, female hosts showed more infection of 1.6% as compared to males with 1.16% in Degema, Nigeria and in Tanzania (Nyenke et al, 2008).…”

Section: Relationship Between Sex and E Histolytica/edispar/emoshksupporting

confidence: 84%

Human amoebiasis in Multan, Punjab, Pakistan

Tasawar¹,

Lashari²,

Anjum³

et al. 2013

J. Cell Anim. Biol.

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To determine the overall prevalence of human amoebiasis at Nishtar Hospital Multan, Punjab, stool samples (n = 766) were collected from patients visiting Nishtar Hospital Multan. Temporary mounts were prepared for determine amoebiasis. Out of 766 patients, 66 were infected with Entamoeba histolytica/Entamoeba dispar/Entamoeba moshkovskii. The overall prevalence of parasites was 8.61%. It was more prevalent in females (9.46%) as compared to males (8.15%). The relationship between sex and human amoebiasis was non-significant (P>0.05). The prevalence of amoebiasis was highest in age group of 33 to 48 years (16.67%) and lowest in 49 to 63 years (3.45%). The difference was statistically significant (P<0.05). The high prevalence may be attributed to low sanitary conditions in the region. The protozoan parasites did not show gender specific differences.

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Section: Relationship Between Sex and E Histolytica/edispar/emoshksupporting

confidence: 84%

Human amoebiasis in Multan, Punjab, Pakistan

Tasawar¹,

Lashari²,

Anjum³

et al. 2013

J. Cell Anim. Biol.

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“…ELISA is the most popular assay in diagnostic laboratories throughout the world and has been used to study the epidemiology of asymptomatic disease (66) and the diagnosis of symptomatic amebiasis after fecal examination. This method is widely thought to be sufficient for clinical purposes, particularly for diagnosis of patients with ALA, and can be easily performed in a clinical laboratory.…”

Section: Antibody Detection Testsmentioning

confidence: 99%

Laboratory Diagnostic Techniques for Entamoeba Species

et al. 2007

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SUMMARY The genus Entamoeba contains many species, six of which (Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, Entamoeba polecki, Entamoeba coli, and Entamoeba hartmanni) reside in the human intestinal lumen. Entamoeba histolytica is the causative agent of amebiasis and is considered a leading parasitic cause of death worldwide in humans. Although recent studies highlight the recovery of E. dispar and E. moshkovskii from patients with gastrointestinal symptoms, there is still no convincing evidence of a causal link between the presence of these two species and the symptoms of the host. New approaches to the identification of E. histolytica are based on detection of E. histolytica-specific antigen and DNA in stool and other clinical samples. Several molecular diagnostic tests, including conventional and real-time PCR, have been developed for the detection and differentiation of E. histolytica, E. dispar, and E. moshkovskii in clinical samples. The purpose of this review is to discuss different methods that exist for the identification of E. histolytica, E. dispar, and E. moshkovskii which are available to the clinical diagnostic laboratory. To address the need for a specific diagnostic test for amebiasis, a substantial amount of work has been carried out over the last decade in different parts of the world. The molecular diagnostic tests are increasingly being used for both clinical and research purposes. In order to minimize undue treatment of individuals infected with other species of Entamoeba such as E. dispar and E. moshkovskii, efforts have been made for specific diagnosis of E. histolytica infection and not to treat based simply on the microscopic examination of Entamoeba species in the stool. The incorporation of many new technologies into the diagnostic laboratory will lead to a better understanding of the public health problem and measures to control the disease.

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“…2 Because they are morphologically indistinguishable, studies based on stool surveys have raised the question on the validity of most of these studies. Moreover, seroepidemiologic studies that were carried out in a number of endemic countries [3][4][5][6][7][8] usually reflected the seroprevalence of the disease even in the near past. Also, the major problem with current serologic test results is that they remain positive for years after an episode of amebiasis.…”

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confidence: 99%

Field study on the distribution of Entamoeba histolytica and Entamoeba dispar in the northern Philippines as detected by the polymerase chain reaction.

Rivera

Tachibana²,

Kanbara³

1998

The American Journal of Tropical Medicine and Hygiene

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Abstract. We used the polymerase chain reaction (PCR) to study the distribution of Entamoeba histolytica and E. dispar in 1,872 individuals in 14 communities in the northern Philippines. Here we report a field study using a DNA extraction protocol from formalin-fixed stool specimens as previously reported. This assay detected 137 stools (7.318%) containing E. dispar and 18 stools (0.961%) containing E. histolytica. The most affected age group for E. histolytica/E. dispar infections were those 5-14 years of age. There was no significant difference in the sex distribution of E. histolytica, while in the case of E. dispar, a higher prevalence was observed in females (9.186%) than in males (5.731%) (P Ͻ 0.01). An apparent clustering of stool-positive cases of E. histolytica and E. dispar was also observed in the northern part of the study area. The results of this survey demonstrate that E. dispar is highly prevalent in the communities studied. Moreover, it offers promise for the PCR using DNA extracted from formalin-fixed stools as a sensitive epidemiologic tool for detecting E. histolytica and E. dispar infections.The intestinal protozoan parasite Entamoeba histolytica is the causative agent of human amebiasis. Entamoeba histolytica is responsible for up to 100,000 deaths per year, placing it second only to malaria in mortality due to protozoan parasites.1 The infection is common in developing countries and predominantly affects individuals with poor socioeconomic conditions, nonhygienic practices, and malnutrition.A number of epidemiologic studies of E. histolytica infection were performed before the clear distinction of two separate species, E. dispar and E. histolytica, was established.2 Because they are morphologically indistinguishable, studies based on stool surveys have raised the question on the validity of most of these studies. Moreover, seroepidemiologic studies that were carried out in a number of endemic countries 3-8 usually reflected the seroprevalence of the disease even in the near past. Also, the major problem with current serologic test results is that they remain positive for years after an episode of amebiasis. To date, the standardization of antigen preparation and the relative measurement of positive cut-off antibody titers for use in serodiagnosis still pose a problem.In recent years, a number of methods such as isoenzyme typing, 9 DNA probes for the polymerase chain reaction (PCR) and hybridization, 10-13 restriction pattern analysis, [14][15][16][17][18][19] and monoclonal antibodies [20][21][22][23][24] have been developed for the clear distinction of the two species. Clearly, accurate diagnostic tools are required for the clinical and public health management of the disease. The use of a stool ELISA has recently been shown to be useful in routine diagnostic procedure and epidemiologic studies. 25,26 However, a comparative study on the use of the ELISA and PCR for the detection of E. histolytica and E. dispar indicated that the PCR was more advantageous than the ELISA in epidemiologic st...

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Prevalence of antibodies againstEntamoeba histolyticain Mexico measured by ELISA

Cited by 14 publications

References 9 publications

Human amoebiasis in Multan, Punjab, Pakistan

Human amoebiasis in Multan, Punjab, Pakistan

Laboratory Diagnostic Techniques for Entamoeba Species

Field study on the distribution of Entamoeba histolytica and Entamoeba dispar in the northern Philippines as detected by the polymerase chain reaction.

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