Prevalence of antibodies to hepatitis E virus among Japanese blood donors: Identification of three blood donors infected with a genotype 3 hepatitis E virus

Fukuda, Satoko; Sunaga, J.; Saito, Nobuo; Fujimura, Kuniko; Itoh, Yaeko; Sasaki, Masahide; Tsuda, Fumio; Takahashi, Masaharu; Nishizawa, Tsutomu; Okamoto, Hiroaki

doi:10.1002/jmv.20125

Cited by 69 publications

(54 citation statements)

References 50 publications

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“…This is the first report that investigated the prevalence of HEV antibody in hemophilia patients in Japan. The prevalence of HEV antibody in our study patients (16.3%) was higher than that previously reported in Japanese blood donors (3.7%) [29] and in Japanese patients undergoing hemodialysis (9.4%) [20] ( table 4 ). The gradual increase in the prevalence of HEV antibody between healthy blood donors, patients undergoing hemodialysis, and patients with hemophilia suggests a possible role of parenteral transmission of HEV.…”

Section: Discussioncontrasting

confidence: 54%

Prevalence of Hepatitis E Virus IgG Antibody in Japanese Patients with Hemophilia

Toyoda

Honda²,

Hayashi³

et al. 2008

Intervirology

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Objective: We investigated the prevalence of antibody against hepatitis E virus (HEV) in Japanese patients with hemophilia. Methods: IgG antibody against HEV was measured in serum of 80 Japanese patients with hemophilia by enzyme-linked immunosorbent assay. The prevalence of HEV antibody was compared with the reported prevalence of HEV antibody in Japanese patients undergoing hemodialysis and in Japanese healthy blood donors. Characteristics of patients and coinfection with other transfusion-transmissible viruses were compared in patients with and without HEV antibody. Results: Anti-HEV IgG antibody was detected in 13 of 80 patients (16.3%). The prevalence was far higher than that reported in Japanese blood donors (3.7%) and was higher than that in Japanese patients undergoing hemodialysis (9.4%). The patients with HEV antibody were significantly older than those without. HEV antibody was not detected in patients <20 years of age and in patients who had received only virus-inactivated coagulation factors. No association was observed between positivity for anti-HEV antibody and severity of hemophilia or coinfection with other parenterally transmissible viruses. Conclusion: Our results suggest that the parenteral transmission of HEV may have occurred in Japanese patients with hemophilia via non-virus-inactivated coagulation factors.

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Section: Discussioncontrasting

confidence: 54%

Prevalence of Hepatitis E Virus IgG Antibody in Japanese Patients with Hemophilia

Toyoda

Honda²,

Hayashi³

et al. 2008

Intervirology

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“…The sero-prevalence of the infection in developing countries with poor sanitation as well as in developed countries has been reported to be respectively higher and lower than Iran (17)(18)(19). Abe et al (2006) reported that the sero-prevalence of hepatitis E in Europe, Brazil, Japan, China, and the Middle East is respectively 0.95% -20.6%, 2.3%, 3.7%, 32.6%, and 4% -5.2% (20).…”

Section: Discussionmentioning

confidence: 99%

Investigating the Seroprevalence of Hepatitis E Among Blood Donors Referring to Blood Donation Centers in Birjand County in Iran

2016

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Background: Hepatitis E is among the most important health challenges in many countries. This study aimed at investigating the sero-prevalence of hepatitis E among blood donors in Birjand county, Iran. Methods: This cross-sectional descriptive-analytical study was performed in 2013 -2014 on 340 blood donors who referred to blood donation centers in Birjand county, Iran, for blood donation. The participants were recruited through simple random sampling. A researcher-made questionnaire was used for data collection which included items such as demographic characteristics, past medical history, history of blood transfusion, and the source of drinking water. The validity of the questionnaire was confirmed by an infectious disease specialist and a statistician. A five-milliliter blood sample was obtained from each donor and serological assessment was performed for detecting hepatitis E virus antibody by using the ELISA method. The data were analyzed through running logistic regression analysis using the SPSS software (version 15.0). Results: From 340 participants, 319 cases (93.8%) were male, 256 cases (75.3%) held university degrees, 13 cases (3.8%) had a history of blood transfusion, and 50 cases (14.7%) were anti-hepatitis E positive. The result of serologic test was significantly correlated with the participants' educational status, place of residence, and source of drinking water (P < 0.05). However, the correlation of the test result with the participants' gender, age, employment, and history of blood transfusion was not statistically significant. Conclusions:The prevalence of hepatitis E in the present study was higher than previous studies, denoting the higher prevalence of hepatitis E in the area. Moreover, the prevalence of hepatitis E is lower among blood donors who have higher educational status, live in urban areas, and use piped water.

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“…HEV is not only a problem in developing countries but has also become a concern of developed countries (Christensen et al, 2002;Clemente-Casares et al, 2003;Fukuda et al, 2004). The high seroprevalence despite the rarity of disease morbidity in the developed countries is still unexplained .…”

Section: Discussionmentioning

confidence: 99%

Characterization of hepatitis E-specific cell-mediated immune response using IFN-γ ELISPOT assay

Shata

Barrett

Shire

et al. 2007

Journal of Immunological Methods

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In developing countries, hepatitis E (HEV) and hepatitis A (HAV) are the major causes of acute viral hepatitis with similar fecooral modes of transmission. In contrast to the high seroprevalence of hepatitis A infection, a low seroprevalence of HEV among children in endemic areas has been reported. These data suggest the possibility that silent HEV infection is undiagnosed by the current available methods. Many of the serological tests used for HEV diagnosis have poor specificity and are unable to differentiate among different genotypes of HEV. Moreover, the RT-PCR used for HEV isolation is only valid for a brief period during the acute stage of infection. Cell-mediated immune (CMI) responses are highly sensitive, and long lasting after sub-clinical infections as shown in HCV and HIV. Our objective was to develop a quantitative assay for cell-mediated immune (CMI) responses in HEV infection as a surrogate marker for HEV exposure in silent infection. Quantitative assessment of the CMI responses in HEV will also help us to evaluate the role of CMI in HEV morbidity. In this study, an HEV-specific interferon-gamma (IFN-γ) ELISPOT assay was optimized to analyze HEV-specific CMI responses. We used peripheral blood mononuclear cells (PBMC) and sera from experimentally infected chimpanzees and from seroconverted and control human subjects to validate the assay. The HEV-specific IFN-γ ELISPOT responses correlated strongly and significantly with anti-HEV ELISA positive/negative results (rho=0.73, p=0.02). Moreover, fine specificities of HEV-specific T cell responses could be identified using overlapping HEV ORF2 peptides.

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Prevalence of antibodies to hepatitis E virus among Japanese blood donors: Identification of three blood donors infected with a genotype 3 hepatitis E virus

Cited by 69 publications

References 50 publications

Prevalence of Hepatitis E Virus IgG Antibody in Japanese Patients with Hemophilia

Prevalence of Hepatitis E Virus IgG Antibody in Japanese Patients with Hemophilia

Investigating the Seroprevalence of Hepatitis E Among Blood Donors Referring to Blood Donation Centers in Birjand County in Iran

Characterization of hepatitis E-specific cell-mediated immune response using IFN-γ ELISPOT assay

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