Prevalence of antimicrobial resistance and molecular characterization of tetracycline resistance mediated by tet(M) and tet(L) genes in Enterococcus spp. isolated from food in Southern Brazil

Frazzon, Ana Paula Guedes; Gama, Bianca Almeida; Hermes, Vanessa Stahl; Bierhals, Christine Garcia; Pereira, Rebeca Inhoque; Guedes, Arthur Gomes; d’Azevedo, Pedro Alves; Frazzon, Jeverson

doi:10.1007/s11274-009-0160-x

Cited by 41 publications

(44 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…again, some enterococci are therapeutically used in the treatment of gastroenteritis in humans and animals. On the other hand, enterococci are considered as important nosocomial pathogens, because they cause bacteraemia, endocarditis, urinary tract and neonatal infections (FraNZ et al, 1999;raDu et al, 2001;GOMeS et al, 2008;FraZZON et al, 2010).…”

mentioning

confidence: 99%

The prevalence, vancomycin resistance and virulence gene profiles of Enterococcus species recovered from different foods of animal origin

Elmali

Can

2018

Vet. arhiv

View full text Add to dashboard Cite

In this study, Enterococcus faecium was the most commonly found species with a level of 10.1%, followed by Enterococcus durans (19/246, 7.7%), Enterococcus faecalis (13/246, 5.2%), and Enterococcus hirae (9/246, 3.6%). When the virulence gene profile of isolates was evaluated, gelE was the predominant (25/66, 37.8%) virulence factor in isolates followed by asa1 (22/66, 33.3%), esp (12/66, 18.1%), and cylA (4/66, 6.0%). None of the isolates harboured the hyl gene. In this study, all and/or the majority of the Enterococcus isolates tested were found to be resistant to ampicillin, rifampicin, vancomycin, and erythromycin. however, vancomycin resistance genes, such as vanB and vanC1, were not determined in any of the isolates by multiplex PCr. Only three isolates of E. durans recovered from Surk cheese were found to be carrying the vanA gene. the results demonstrate that antimicrobial-resistant and virulent strains of enterococci occur in food of animal origin in turkey.

show abstract

mentioning

confidence: 99%

The prevalence, vancomycin resistance and virulence gene profiles of Enterococcus species recovered from different foods of animal origin

Elmali

Can

2018

Vet. arhiv

View full text Add to dashboard Cite

show abstract

“…Although tetracycline is an antimicrobial not approved by the European Union as a food supplement for chickens, therapeutic and prophylactic use in veterinary is common, and Enterococcus resistant to tetracycline are frequently found in broilers, animal feed, poultry production environment and chicken carcasses (7,15,20). Dietary supplementations did not influence the resistance profile in enterococcus isolated from broilers.…”

Section: Identification Of Enterococci Isolatesmentioning

confidence: 91%

“…The primer of tet(M), tet(L) and erm(B) genes and PCR amplifications were performed as described by Frazzon et al. (15).…”

Section: Detection Of Tet(m) Tet(l) and Erm(b) Genesmentioning

confidence: 99%

“…Enterococci are gram-positive bacteria widely distributed in the environment, inhabiting mainly the gastrointestinal tract of humans and animals (33). Furthermore, enterococci are opportunistic pathogens commonly associated with in immunocompromised patients and nosocomial infection (15). Enterococcus sp.…”

Section: Introductionmentioning

confidence: 99%

“…Enterococcus sp. resistant to several antimicrobials were isolated from clinical specimens and environment (5,8,15,18,37). These microorganisms are intrinsically resistant against many antimicrobial agents commonly prescribed for Gram-positive cocci and exhibit resistance to a wide variety of other antimicrobials, by acquisition of resistance genes via transposons or plasmids, such as, tetracycline and macrolides (25).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Species distribution and antimicrobial susceptibility of enterococci isolated from broilers infected experimentally with Eimeria spp and fed with diets containing different supplements

Cassenego¹,

d’Azevedo²,

Ribeiro³

et al. 2011

Braz. J. Microbiol.

View full text Add to dashboard Cite

Resistant bacteria in animal can be spread to environment and to humans. Poultry feed and infections caused by Eimeria spp. are important factors in determining the intestinal microbial communities. The aim of this study was to verify the prevalence of species and antimicrobial susceptibility of Enterococcus isolated from broilers fed with different supplements and infected experimentally with Eimeria spp. Broilers were divided in eight groups, fed with diets supplemented with a combination of antimicrobial, ionophore-coccidiostatics, probiotic, essential oil. At 14 days old all birds, except the control, received a solution containing oocysts of Eimeria spp. Samples of cloacal swabs from broilers were collected. A total of 240 Enterococcus sp. strains were isolated, confirmed genus by PCR, classified as species, tested for antimicrobial susceptibility and screened by PCR for the presence of tet(L), tet(M) and erm(B) genes. The overall distribution of species isolated from fecal samples was E. faecalis (40%), followed by E. casseliflavus/E. gallinarum (10.8%), E. mundtii (10.8%), E. faecium (10.8%), E. columbae (5.8%) and E. gallinarum (4.2%). Changes in the composition or frequency of Enterococcus species were observed in all dietary supplementation.Antimicrobial susceptibility tests showed resistance phenotypes a range of antibiotics, especially used in humans such as, streptomycin, penicillin, rifampicin and vancomycin. There was no correlation between different supplementation for broilers and antimicrobial resistance and the presence of tet(M), tet(L) and erm(B) genes. Dietary supplementation had effect on the Enterococcus sp. colonization, but did not have significant effect on the phenotype and genotype of antimicrobial resistance in enterococci.

show abstract

Characterization of tetracycline resistance genes in tetracycline-resistant Enterobacteriaceae obtained from a coliform collection

Sandallı

Özgümüş

Sevim

2010

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Tetracycline resistance has been used as the key determinant to monitor resistance genes in natural environments such as rivers, lakes or seawater. The aim of this study was evaluate the frequency of tetA, tetB, tetC, tetD and tetE genes in 52 tetracycline-resistant Enterobacteriacea isolated from river water in the North East Black Sea Region of Turkey. In 52 tetracycline-resistant strains, resistance was mediated by tetA in eight (15.3%) strains, tetB in ten (19.2%) strains and both tetA and tetB in one (1.9%) strain. No tetC, tetD or tetE-mediated resistance was detected. In conclusion, the river water may be considered as a reservoir for the antibiotic resistance genes and the people living in this area may be under risk. To our knowledge, this is the first report for molecular characterization of tetracycline resistance in Enterobacteriaceae of river water origin in Turkey.

show abstract

Prevalence of antimicrobial resistance and molecular characterization of tetracycline resistance mediated by tet(M) and tet(L) genes in Enterococcus spp. isolated from food in Southern Brazil

Cited by 41 publications

References 29 publications

The prevalence, vancomycin resistance and virulence gene profiles of Enterococcus species recovered from different foods of animal origin

The prevalence, vancomycin resistance and virulence gene profiles of Enterococcus species recovered from different foods of animal origin

Species distribution and antimicrobial susceptibility of enterococci isolated from broilers infected experimentally with Eimeria spp and fed with diets containing different supplements

Characterization of tetracycline resistance genes in tetracycline-resistant Enterobacteriaceae obtained from a coliform collection

Contact Info

Product

Resources

About