Prevalence of Bartonella species, hemoplasmas, and Rickettsia felis DNA in blood and fleas of cats in Bangkok, Thailand

Assarasakorn, Sukullaya; Veir, Julia K.; Hawley, Jennifer; Brewer, Melissa; Morris, Arianne K.; Hill, Ashley E.; Lappin, Michael R.

doi:10.1016/j.rvsc.2012.03.015

Cited by 53 publications

(78 citation statements)

References 30 publications

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“…The flea Ctenocephalides felis is the main ectoparasite incriminated in transmission of hemotropic hemoplasmas among cats worldwide, especially because it is the ectoparasite most commonly found parasitizing these animals (SHAW et al, 2004;LAPPIN et al, 2006;MENDES-DE-ALMEIDA et al, 2007). DNA samples of 'Candidatus M. haemominutum', M. haemofelis and 'Candidatus M. turicensis' have been found in fleas and their feces (SHAW et al, 2004;WOODS et al, 2005;LAPPIN et al, 2006;HORNOK et al, 2010;ASSARASAKORN et al, 2012). Mhf and CMhm DNA was also found in ticks (FYUMAGWA et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Hematological changes associated with hemoplasma infection in cats in Rio de Janeiro, Brazil

Raimundo

Guimarães

Botelho

et al. 2016

Rev. Bras. Parasitol. Vet.

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This study aimed to detect Mycoplasma spp. in naturally infected cats from Rio de Janeiro and to evaluate hematological abnormalities and factors associated with this infection. Out of the 197 cats sampled, 11.2% presented structures compatible with hemoplasma organisms on blood smears. In contrast, 22.8% were positive for Mycoplasma spp. by means of 16S rRNA gene real-time polymerase chain reaction, which reflects the weak concordance between techniques. The infection rates, by means of 16S rRNA gene conventional polymerase chain reaction, was 4.6%, 4.6% and 11.7% for Mycoplasma haemofelis (Mhf), ‘Candidatus Mycoplasma turicensis’ (CMt) and ‘Candidatus Mycoplasma haemominutum’ (CMhm), respectively. Mhf and CMhm infections are more frequent in the summer (p>0.05). Presence of anemia (p < 0.02), lymphocytosis (p < 0.03), thrombocytopenia (p < 0.04) and activated monocytes (p < 0.04) was associated with Mhf infection. No hematological abnormality was associated with CMt or CMhm infection. Male cats were more prone to be infected by Mhf or CMhm (p < 0.01). Adult cats had more chance to be infected by CMhm. Three hemoplasma species occur in the metropolitan region of Rio de Janeiro and Mhf seems to be the most pathogenic of them. Anemia is the most important hematological abnormality.

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Section: Discussionmentioning

confidence: 99%

Hematological changes associated with hemoplasma infection in cats in Rio de Janeiro, Brazil

Raimundo

Guimarães

Botelho

et al. 2016

Rev. Bras. Parasitol. Vet.

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“…In a separate survey, Kosoy and others 15 screened the blood of 261 patients to identify what role Bartonella species play in acute febrile illness in Thailand; Bartonella spp. were detected in 7.7% (20) of these samples. Sequencing demonstrated the presence of rodent-borne Bartonella species in half of these samples, specifically B. rattimassiliensis, B. vinsonii subsp.…”

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confidence: 89%

“…12,13,17 However, little information has been obtained to identify potential arthropod vectors of Bartonella species in Thailand. Bartonella henselae, B. clarridgeiae and B. koehlerae were detected in Ctenocephalides felis fleas removed from cats [18][19][20] and B. henselae was identified in two C. canis 19 also collected from cats. Furthermore, a Bartonella sp., similar to B. grahamii, was found in a rodent flea, Nosopsyllus fasciatus, obtained from Rattus surifer.…”

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confidence: 96%

Molecular Detection and Identification of Bartonella Species in Rat Fleas from Northeastern Thailand

Billeter¹,

Colton²,

Sangmaneedet³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. The presence of Bartonella species in Xenopsylla cheopis fleas collected from Rattus spp. (R. exulans, R. norvegicus, and R. rattus) in Khon Kaen Province, Thailand was investigated. One hundred ninety-three fleas obtained from 62 rats, were screened by polymerase chain reaction using primers specific for the 16S-23S intergenic spacer region, and the presence of Bartonella DNA was confirmed by using the citrate synthase gene. Bartonella DNA was detected in 59.1% (114 of 193) of fleas examined. Sequencing demonstrated the presence of Bartonella spp. similar to B. elizabethae, B. rattimassiliensis, B. rochalimae, and B. tribocorum in the samples tested with a cutoff for sequence similarity 96% and 4 clustered together with the closest match with B. grahamii (95.5% identity). If X. cheopis proves to be a competent vector of these species, our results suggest that humans and animals residing in this area may be at risk for infection by several zoonotic Bartonella species.

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“…The most common Bartonella species detected in cats are B. henselae and B. clarridgeiae, and both have been implicated as the cause of cat scratch disease in humans (Boulouis et al 2005). A third species, B. koehlerae, has been isolated from cats (Droz et al 1999, Rolain et al 2003, Avidor et al 2004, Assarasakorn et al 2012 and was implicated as the cause of endocarditis in a human patient from Israel, and more recently detected in the blood of eight immunocompetent patients from the United States (Avidor et al 2004, Breitschwerdt et al 2010. Another two species, B. bovis and B. quintana, were also detected in cats; however, the information on the distribution of B. koehlerae, B. bovis, and B. quintana within cat populations worldwide is lacking (Boulouis et al 2005, La et al 2005, Chomel et al 2006.…”

Section: Introductionmentioning

confidence: 99%

Bartonellae in Domestic and Stray Cats from Israel: Comparison of Bacterial Cultures and High-Resolution Melt Real-Time PCR As Diagnostic Methods

Gutiérrez

Morick

Gross

et al. 2013

Vector-Borne and Zoonotic Diseases

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To determine the occurrence of feline bartonellosis in Israel, blood samples were collected from 179 stray and 155 domestic cats from 18 cities or villages in central and northcentral Israel. Samples were screened for Bartonella infection by culture isolation and molecular detection using high-resolution melt (HRM) real-time PCR assay targeting the 16S-23S rRNA internal transcribed spacer (ITS). All positive samples were confirmed by two additional HRM real-time PCR assays targeting two fragments of the β-subunit of RNA polymerase (rpoB) and the 16S rRNA genes. The prevalence of Bartonella spp. infection in the general tested population was 25.1% (84/334). A higher prevalence was detected in the stray (30.7%; 55/179) than the domestic cats (18.7%; 29/155). Bartonella henselae, Bartonella clarridgeiae, and Bartonella koehlerae were highly prevalent in both cat populations, however their distribution among the two populations varied significantly (p=0.016). B. clarridgeiae and B. koehlerae were found to be more prevalent in stray than domestic cats, whereas B. henselae was evenly distributed. Co-infection with two or more different Bartonella spp. was determined in 2.1% (7) of the cats. The ITS HRM real-time PCR assay used in this study was shown to have a greater screening power than bacterial isolation, detecting 94.0% (79/84) compared to 35.7% (30/84), respectively, of all positive samples. The high prevalence of these zoonotic Bartonella species, coupled with the overpopulation of stray cats, and increased numbers of domestic cats in the major urban centers in Israel represent a significant threat for the public health in this country.

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Prevalence of Bartonella species, hemoplasmas, and Rickettsia felis DNA in blood and fleas of cats in Bangkok, Thailand

Cited by 53 publications

References 30 publications

Hematological changes associated with hemoplasma infection in cats in Rio de Janeiro, Brazil

Hematological changes associated with hemoplasma infection in cats in Rio de Janeiro, Brazil

Molecular Detection and Identification of Bartonella Species in Rat Fleas from Northeastern Thailand

Bartonellae in Domestic and Stray Cats from Israel: Comparison of Bacterial Cultures and High-Resolution Melt Real-Time PCR As Diagnostic Methods

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