Prevalence of Brucella spp in humans

Soares, Catharina de Paula Oliveira Cavalcanti; Teles, José Andreey Almeida; Santos, Aldenir Feitosa dos; Silva, Stemberg Oliveira Firmino; Cruz, Maria Vilma Rocha Andrade; SilvaJunior, F. F.

doi:10.1590/0104-1169.0350.2632

Cited by 17 publications

(27 citation statements)

References 21 publications

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“…There has been extensive scientific research that has shown a wide range of zoonotic infections, which are caused by different strains of Brucella ( 31 – 34 ). The identification of these bacteria can be very critical in the medical society ( 35 ). Because of some antibiotic-resistant genes, the prevalence of Brucellosis is increasing, and, as a result, it is necessary to develop a rapid identification technique that can replace culture and biochemical tests.…”

Section: Resultsmentioning

confidence: 99%

Microarray-based long oligonucleotides probe designed for Brucella Spp. detection and identification of antibiotic susceptibility pattern

et al. 2016

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Brucella spp. is a common zoonotic infection referred to as Brucellosis, and it is a serious public health problem around the world. There are currently six classical species (pathogenic species in both animals and humans) within the genus Brucella. The ability and practicality facilitated by a microarray experiment help us to recognize Brucella spp. and its antibiotic resistant gene. Rapid phenotypic determination of antibiotic resistance is not possible by disk diffusion methods. Thus, evaluating antibiotics pattern and Brucella detection appear necessary technique by molecular methods in brucellosis. So, the aim of this study was to design a microarray long oligonucleotides probe and primer for the complete diagnosis of Brucella spp. and obtaining genetic profiles for antibiotic resistance in bacteria at the same time. In this study, we designed 16 antibiotic-resistant gene solid-phase primers with similar melting temperatures of 60 °C and 16 long oligonucleotide probes. These primers and probes can identify tetracycline-, chloramphenicol-, and aminoglycoside-resistant genes, respectively. The design of microarray probes is a versatile process that be done in a wide range of selections. Since the long oligo microarray probes are the best choices for specific diagnosis and definite treatment, this group of probes was designed in the present survey.

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Section: Resultsmentioning

confidence: 99%

Microarray-based long oligonucleotides probe designed for Brucella Spp. detection and identification of antibiotic susceptibility pattern

et al. 2016

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“…Respecto a este último factor, el consumo de lácteos no pasteurizados continúa siendo una de las principales causas de infección (Swai & Schooman 2009;Yoo et al 2009;Cavalcanti et al 2015;Mufinda et al 2017;Acharya et al 2018). A pesar de que, en el área de estudio, la práctica de consumir productos lácteos sin pasteurizar es muy frecuente y es un importante factor de riesgo, se considera importante hacer notar que el consumo de leche, que se esperaba fuera un factor de riesgo, no lo es (Cavalcanti et al 2015). Esto se explica en que, en los hogares de los encuestados, la leche suele ser hervida para el consumo en su forma fluida, más no para la producción de quesos artesanales, helados, yogurt u otros.…”

Section: Discussionunclassified

Aspectos epidemiológicos de la brucelosis en humanos en las Áreas Rectoras Aguas Zarcas y Los Chiles, Costa Rica, 2015-2017

Galeano¹,

Mojica²,

Rojas³

et al. 2020

Ciencias Veterinarias

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La brucelosis es una enfermedad zoonótica causada por especies del género Brucella spp., con baja notificación y subregistro en Costa Rica, especialmente en personas y en bovinos. Se determina la seroprevalencia para Brucella spp. lisas y factores de riesgo asociados, en personas que habitan o trabajan en fincas ganaderas positivas a brucelosis bovina de las Áreas Rectoras de Salud de Aguas Zarcas y Los Chiles, Alajuela, entre 2015 y 2017, mediante un estudio transversal analítico. Se toma una muestra sanguínea a las personas estudiadas y se les aplica una encuesta, previo consentimiento informado. Las muestras son analizadas, en paralelo, mediante las pruebas de Rosa de Bengala y sero aglutinación (SAT). Se realiza estadística descriptiva de los factores personales, culturales y ambientales, asociados con el riesgo a brucelosis. Además, se estima razones de posibilidades (Odds ratio) mediante regresión logística no condicional, para determinar factores asociados con su estado serológico. La seroprevalencia para Brucella spp. lisas es 12.5%. La elaboración de alimentos con productos lácteos no pasteurizados (OR=11.0; IC 95%: 3.0-39.9); la inseminación artificial sin protección adecuada (OR=8.84; IC 95%: 1.8-43.5); la atención de partos sin protección básica (OR=3.9; IC 95%: 1.1-14.1) y el consumo de helados caseros, fabricados con leche sin pasteurizar (OR=7.9; IC 95%: 2.7-22.6), representan los principales factores de riesgo. Otros factores muestran tendencia al riesgo, pero es posible que la baja muestra haya afectado la potencia estadística de las estimaciones. Se demuestra una importante exposición a Brucella spp. lisas en quienes laboran y viven en fincas de ganado bovino, con animales seroreactores a Brucella abortus, por la realización de prácticas culturales de riesgo. La diferencia cuantitativa, entre los casos notificados al sistema de vigilancia epidemiológica del Ministerio de Salud (Ministerio de Salud 2015; Ministerio de Salud 2016), con los resultados de este estudio, indican que se debe reforzar la inclusión de esta enfermedad como diagnóstico diferencial para casos de enfermedad febril aguda, e instaurar un sistema de vigilancia epidemiológica eficaz y eficiente.

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“…(PESSEGUEIRO et al, 2003). This genus presents ten species that have as their preferential hosts: Brucella microti (infects field mice), Brucella inopinata (infects humans), Brucella abortus (infects cattle), Brucella pinnipedialis (infects marine mammals that alternate their habitat between sea and land, for example, walrus and seals), Brucella melitensis (infects goats), Brucella suis (infects reindeer, pigs, hares, and rodents), Brucella ceti (infects cetaceans), Brucella ovis (infects sheep), Brucella neotomae (infects desert rats), and Brucella canis (infects dogs) (SOARES et al, 2015).…”

Section: Seroprevalence Of Ovine Brucellosis In the Microregion Of Tementioning

confidence: 99%

Seroprevalence of ovine brucellosis in the microregion of Teresina, Piauí, Brazil

et al. 2020

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Among the diseases that affect the reproductive system of domestic animals, brucellosis in the sheep species is important because it generates significant economic losses to sheep rearing. Thus, it is a threat to the growth and productivity of sheep herds. In the face of this problem, the objective of the present research was to identify the prevalence of ovine brucellosis in herds in municipalities of the Teresina, Piauí, Brazil microregion by using the agar gel immunodiffusion assay (AGID) and indirect enzyme-linked immunosorbent assay (ELISA) serological tests. Fourteen municipalities were included in the research. Blood samples were collected from 521 pubescent animals by puncturing the jugular vein. After collection, the samples were submitted to the serological techniques, AGID and indirect ELISA, to detect anti B. ovis antibody. Of the 521 samples submitted to the AGIDtest, 23 (4.41%) were sera reagent and 498 (95.58%) negative. The indirect ELISA tests, 24 (4.61%) suspect samples and 497 (95.39%) negative samples were obtained, and there were no reagent animals in this test, only suspect. The seroprevalence of ovine brucellosis in the Teresina, Piauí microregion was 4.41%. Thus, it is possible to identify sheep with reagent serology to infection by B. ovis, that is present in municipalities in the state of Piauí, Brazil. Furthermore, AGIDwas shown to be more sensitive in detecting animals that had had contact with the etiological agent of the disease.

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Prevalence of Brucella spp in humans

Cited by 17 publications

References 21 publications

Microarray-based long oligonucleotides probe designed for Brucella Spp. detection and identification of antibiotic susceptibility pattern

Microarray-based long oligonucleotides probe designed for Brucella Spp. detection and identification of antibiotic susceptibility pattern

Aspectos epidemiológicos de la brucelosis en humanos en las Áreas Rectoras Aguas Zarcas y Los Chiles, Costa Rica, 2015-2017

Seroprevalence of ovine brucellosis in the microregion of Teresina, Piauí, Brazil

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