Prevalence of cagA and vacA genes in isolates from patients with Helicobacter pylori-associated gastroduodenal diseases in Recife, Pernambuco, Brazil

Brito, Carlos; Silva, Lenôra M B; Jucá, Norma Thomé; Leal, Nilma Cintra; Souza, Wayner Vieira de; Queiroz, Dulciene Maria Magalhães; Cordeiro, Francisco; Silva, Norma Lucena

doi:10.1590/s0074-02762003000600018

Cited by 25 publications

(21 citation statements)

References 27 publications

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“…In this study, infection by virulent strains (CagA+) was greater in patients with peptic ulcers and adenocarcinoma than in patients with gastritis or in subjects in the control group. Similar results were found in a previous study carried out in Belém and other Brazilian states, where the presence of H pylori CagA+ strains was associated with the development of peptic ulcers and adenocarcinoma [28,29] . The frequency of polymorphisms in genes IL-1β-31 and IL-1β-511 in our study was similar to that described in Caucasian [7,24] and Asiatic populations [23,30] , whereas the frequency of polymorphisms in genes IL-1RN and TNF-α-308 was significantly different from that reported in Asiatic [23] and Caucasian [7,24] populations, respectively.…”

Section: Il-1rn and Tnf-α And The Presence Of Infection Bysupporting

confidence: 91%

“…The patients with adenogastric carcinoma were older than those in the control group. These patients had been developing progressive gastric cancer lesions for a long time [29] . Some studies have demonstrated that polymorphisms in genes IL-1β, IL-1RN and TNF-α, together with H pylori infection are associated with an increased risk of developing stomach cancer [7,10,11] ; therefore, to better understand the factors related to the high prevalence of stomach cancer in our region we analyzed the frequency of the genotypes of polymorphisms in genes IL-1β, 1 Queiroz et al [25] 2004; 2 El-Omar et al [7] 2000; 3 El-Omar et al [24] 2003; 4 Zeng et al [23] 2003; 5 Yea et al [11] 2001.…”

Section: Discussionmentioning

confidence: 99%

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Interleukin-1 and TNF-α polymorphisms and Helicobacter pylori in a Brazilian Amazon population

Barbosa¹,

Martins²,

Santos³

et al. 2009

WJG

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RESULTS:A comparison of the frequencies of the different polymorphisms studied among the patients and the control group demonstrated that the allele IL-1RN*2 was more frequent among patients with gastric ulcers and adenocarcinoma. Carriers of the allele IL-RN*2 and those with reactive serology for anti-CagA IgG had a greater risk of developing peptic ulcer and gastric adenocarcinoma, as well as a higher degree of inflammation and neutrophilic activity in the gastric mucosa. CONCLUSION:Our results indicate a positive association between IL-1RN gene polymorphism and infection by positive H pylori CagA strains and the development of gastric ulcers and adenocarcinoma.

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Section: Il-1rn and Tnf-α And The Presence Of Infection Bysupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Interleukin-1 and TNF-α polymorphisms and Helicobacter pylori in a Brazilian Amazon population

Barbosa¹,

Martins²,

Santos³

et al. 2009

WJG

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“…Previous studies conducted in the State of Pará reported a higher prevalence of this genotype in patients with different gastrointestinal diseases (17,31,37,38) . The same was observed in studies conducted in other Brazilian states such as Belo Horizonte (Minas Gerais) (2) , Salvador (Bahia) (8) , and Botucatu (São Paulo) (33) . Analysis of the genotypes of H. pylori contributes to explain the distribution of gastrointestinal diseases around the world, given the fact that, although the prevalence of H. pylori infection is higher in developing countries, some developed countries such as Japan, Portugal and Spain have high frequencies of severe gastrointestinal diseases such as peptic ulcers and gastric cancer.…”

Section: Frequency Of Different Genotypes In Patients With Gastrointesupporting

confidence: 81%

“…These data indicate a greater association between infection with bacterial strains carrying this genotype (s1m1cagA+) and an increased risk of developing more severe forms of infection. This association is supported by studies conducted in Brazil and in other countries (7,8,17,33,37,39) . With respect to the association of different alleles of the vacA gene and presence of the cagA gene with histopathological findings, we observed that patients infected with s1m1cagA+ strains exhibited a high degree of inflammation and neutrophil activity in the gastric mucosa, as well as an increased risk of developing intestinal metaplasia.…”

Section: Frequency Of Different Genotypes In Patients With Gastrointementioning

confidence: 53%

Helicobacter Pylori Infection in Patients With Different Gastrointestinal Diseases From Northern Brazil

Vinagre

Queiroz

Júnior³

et al. 2015

Arq. Gastroenterol.

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-Background -The mechanisms whereby Helicobacter pylori produces different pathological manifestations in the stomach and duodenum are not fully understood. Considering the geographic diversity in the prevalence of virulence factors of this microorganism and their association with the development of different diseases, the search for pathogenicity markers such as CagA and VacA alleles by molecular techniques has intensified. Objectives -To investigate the presence of H. pylori infection and the frequency of different genotypes of this bacterium in patients with gastrointestinal diseases from Northern Brazil, and to establish their association with the histopathological findings. Methods -In a prospective study, samples were collected from 554 patients with different gastrointestinal diseases (gastritis, duodenal ulcer, gastric ulcer, and gastric cancer) seen at a referral hospital attending the entire State of Pará, located in the metropolitan region of Belém. Data such as gender and age obtained with an epidemiological questionnaire were analyzed. The presence of H. pylori and the bacterial genotype were investigated by PCR. Gastric biopsies were assessed histologically. Results -The prevalence of H. pylori infection was 91%. Infection was more frequent among patients with gastric ulcer and gastric cancer. In these groups, there was a predominance of men and older patients when compared to the other two groups studied. The predominant bacterial genotype was s1m1cagA+, which was more frequent among patients with gastric ulcer, duodenal ulcer and gastric cancer. A significant association was observed between s1m1cagA+ strains and a higher degree of inflammation, neutrophil activity and development of intestinal metaplasia. Conclusion -The present study demonstrates a high incidence of H. pylori infection in the patients analyzed, especially among those with gastric ulcer and gastric cancer. Virulent s1m-1cagA+ strains predominated and were associated with more severe lesions. HEADINGS -Helicobacter pylori. Helicobacter infections. Gastrointestinal diseases. Genotype.

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“…In Brazil, the prevalence of H. pylori infection may be as high as 80% in adults, whereas the prevalence is typically 30-70% in North America and Europe (Souto et al 1998, Mitchell 1999, Brito et al 2003, Mitchell et al 2003, Tseng et al 2006, Yamaoka 2008. Despite these dramatic infection rates, the modes of acquisition and transmission of H. pylori remain unclear.…”

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confidence: 99%

Helicobacter pylori detection in gastric biopsies, saliva and dental plaque of Brazilian dyspeptic patients

Rasmussen

Lábio

Gatti

et al. 2010

Mem. Inst. Oswaldo Cruz

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Helicobacter pylori is an important human pathogen that causes chronic gastritis and is associated with the development of peptic ulcer disease and gastric malignancies. The oral cavity has been implicated as a potential H. pylori reservoir and may therefore be involved in the reinfection of the stomach, which can sometimes occur following treatment of an H. pylori infection. The objectives of this paper were (i) to determine the presence of H. pylori in the oral cavity and (ii) to examine the relationship between oral H. pylori and subsequent gastritis. Gastric biopsies, saliva samples and dental plaques were obtained from 78 dyspeptic adults. DNA was extracted and evaluated for the presence of H. pylori using polymerase chain reaction and Southern blotting methods. Persons with gastritis were frequently positive for H. pylori in their stomachs (p < 0.0001) and there was a statistically significant correlation between the presence of H. pylori in gastric biopsies and the oral cavity (p < 0.0001). Our results suggest a relationship between gastric infection and the presence of this bacterium in the oral cavity. Despite this, H. pylori were present in the oral cavity with variable distribution between saliva and dental plaques, suggesting the existence of a reservoir for the species and a potential association with gastric reinfection

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Prevalence of cagA and vacA genes in isolates from patients with Helicobacter pylori-associated gastroduodenal diseases in Recife, Pernambuco, Brazil

Abstract: Geographical differences in the prevalence of

Cited by 25 publications

References 27 publications

Interleukin-1 and TNF-α polymorphisms and Helicobacter pylori in a Brazilian Amazon population

Interleukin-1 and TNF-α polymorphisms and Helicobacter pylori in a Brazilian Amazon population

Helicobacter Pylori Infection in Patients With Different Gastrointestinal Diseases From Northern Brazil

Helicobacter pylori detection in gastric biopsies, saliva and dental plaque of Brazilian dyspeptic patients

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