Prevalence of Capnocytophaga canimorsus in dogs and occurrence of potential virulence factors

Mally, Manuela; Paroz, Cécile; Shin, Hwain; Meyer, Salome; Soussoula, Lavinia V.; Schmiediger, Ueli; Saillen-Paroz, Caroline; Cornelis, Guy R.

doi:10.1016/j.micinf.2009.02.005

Cited by 40 publications

(40 citation statements)

References 20 publications

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“…The authors suggested that they could differentiate C. canimorsus and C. cynodegmi on colony morphology; this is not supported by the original colony description (3) and is not our experience. Mally et al (15) found that the combined yield for both species, after correction for the subcluster, is 41%, which is comparable to our combined yield of 50%. Whereas we could confirm that C. canimorsus is present in the mouths of most dogs, we could not confirm that these dogs harbored the same genotype.…”

Section: Discussionsupporting

confidence: 87%

“…In a recent paper, the presence of C. canimorsus in dogs by culture was estimated at 60% (15). However, these authors used rrs gene sequencing and subsequent cluster analysis to discriminate between C. canimorsus and C. cynodegmi.…”

Section: Discussionmentioning

confidence: 99%

“…The authors did not report whether they also looked for double infections by screening multiple colonies from every dog. In a very recent paper, Capnocytophaga species strains, characterized as C. canimorsus by rrs sequence analysis, were cultured from 61% of 103 dogs (15). However, rrs sequence analysis might not be the most appropriate way to distinguish between C. canimorsus and C. cynodegmi.…”

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confidence: 99%

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Molecular Characterization of Capnocytophaga canimorsus and Other Canine Capnocytophaga spp. and Assessment by PCR of Their Frequencies in Dogs

et al. 2009

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Capnocytophaga canimorsus can be a virulent pathogen, whereas C. cynodegmi is of low virulence. Heterogeneity within these species, their frequency in dogs, and pathogenicity factors are largely unknown. Strains from blood cultures from patients presumptively identified as C. canimorsus (n ‫؍‬ 25) and as C. cynodegmi by rrs analysis (n ‫؍‬ 4), blood cultures from dogs (n ‫؍‬ 8), blood cultures from cats (n ‫؍‬ 2), and cultures from swabs from dog mouths (n ‫؍‬ 53) were analyzed. PCR-restriction fragment length polymorphism (PCR-RFLP), a species-specific PCR on rpoB, and rrs sequencing were used. All 29 strains from human blood cultures could be grouped into three PCR-RFLP types. One included the C. canimorsus type strain, and the other types were closely related. Two canine strains were C. canimorsus and grouped into the least common RLFP pattern group. Five were C. cynodegmi and clustered with the reference strain. One canine and both feline strains were distinct. Four human strains that presumptively had been identified as C. cynodegmi by RNA gene sequence analysis clustered with the C. canimorsus strains by both PCR-RFLP and the sequence-specific PCR of the rpoB gene. C. canimorsus DNA was present in 73% (range, 61 to 85%) of dogs' mouths, and C. cynodegmi DNA was present in 96% (range, 94 to 100%) of dogs' mouths. As defined by rpoB PCR-RFLP and by PCRs using specific primers, all strains from human blood were C. canimorsus. The sequencing of rrs genes suggested the presence of different gene copies in a few strains, indicating that the method is less appropriate for species identification. Both species are present in the majority of dogs. Additional Capnocytophaga species occur in dogs' and cats' mouths.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Molecular Characterization of Capnocytophaga canimorsus and Other Canine Capnocytophaga spp. and Assessment by PCR of Their Frequencies in Dogs

et al. 2009

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“…Lipid A and LPS fractions were eluted using a gradient of methanol/chloroform/water (57:12:31, v/v/v) containing 10 mM NH 4 OAc as mobile phase A and chloroform/ methanol (70.2:29.8, v/v) with 50 mM NH 4 OAc as mobile phase B. The initial solvent system consisted of 2% B and was maintained for 20 min, followed by a three-step linear gradient rising from 2 to 17% B (20 -50 min), from 17 to 27% B (50 -85 min), and from 27 to 100% B (85-165 min).…”

Section: Preparation and Hplc Purification Of The Intact Rough-type Lpsmentioning

confidence: 99%

NMR-based Structural Analysis of the Complete Rough-type Lipopolysaccharide Isolated from Capnocytophaga canimorsus

Zähringer

Ittig

Lindner

et al. 2014

Journal of Biological Chemistry

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Background: Rough-type LPS of C. canimorsus is biologically active, whereas lipid A is not. Results: Purified C. canimorsus rough-type LPS could be analyzed in intact form by NMR. Conclusion: Inactive lipid A becomes active in case the core oligosaccharide is attached. Significance: This study provides evidence that lipid A is not the sole part of the LPS structure responsible for endotoxic activity.

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“…Therefore, to develop more convenient and specific PCR systems to identify the Capnocytophaga spp. is required [11] .…”

Section: Introductionmentioning

confidence: 99%

Detection of Capnocytophaga canimorsus and Capnocytophaga cynodegmi by Cultural and Molecular Methods in Dogs in Western Turkey

Özavcı¹,

Kırkan²

2013

Kafkas Univ Vet Fak Derg

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The aim of this study is to exhibit the presence of Capnocytophaga species in Turkey and to determine the regional risks of these bacteria for the people in dogs. Totally 200 oral swab samples were taken from owned dogs which have had dental plaque problems. The diagnosis of Capnocytophaga infections were done by PCR using CaL2, AS1, CaR and CyR gene sequence primers. The first PCR was carried out to samples using by CaL2 and AS1 primers and results were estimated as Capnocytophaga species. The second PCR was applied to samples using CaR and CyR reverse primers and results were also interpreted as being Capnocytophaga canimorsus and Capnocytophaga cynodegmi. At the end of the first PCR, Capnocytophaga spp. was detected from 11 (5.5%) out of 200 samples. CaR and CyR gene were investigated in samples which were detected as Capnocytophaga spp. It was determined that 2 (18.2%) of the samples were positive for CaR gene which were identified as C. canimorsus and 9 (81.8%) of the samples were positive for both CaR and CyR gene which were identified as C. canimorsus and C. cynodegmi. C. canimorsus and C. cynodegmi species are concluded for generating risk for human health and due to the lacking of information about the disease it was also difficult to diagnosis these agents.

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Prevalence of Capnocytophaga canimorsus in dogs and occurrence of potential virulence factors

Cited by 40 publications

References 20 publications

Molecular Characterization of Capnocytophaga canimorsus and Other Canine Capnocytophaga spp. and Assessment by PCR of Their Frequencies in Dogs

Molecular Characterization of Capnocytophaga canimorsus and Other Canine Capnocytophaga spp. and Assessment by PCR of Their Frequencies in Dogs

NMR-based Structural Analysis of the Complete Rough-type Lipopolysaccharide Isolated from Capnocytophaga canimorsus

Detection of Capnocytophaga canimorsus and Capnocytophaga cynodegmi by Cultural and Molecular Methods in Dogs in Western Turkey

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