Prevalence of catheter associated biofilm producing bacteria and their antibiotic sensitivity pattern

Almalki, Mohammed A.; Varghese, Rakesh

doi:10.1016/j.jksus.2019.11.037

Cited by 36 publications

(21 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As expected, HAI events were more frequent in the ICUs (37.2%) compared with non-ICU locations [HAI events in wards and outpatients were 32.9% and 29.9%, respectively], a finding which was previously described in local studies [ 10 , 28 ] and may reflect the epicenter role of ICU in both infections and antimicrobial resistance. The predominant isolates to cause HAIs were gram-negative organisms (71.9% vs. 28.1%); this finding was similar with many Saudi studies made in different cities in Saudi Arabia including Riyadh [ 6 , 28 , 29 ], Makkah [ 30 , 31 ], Dhahran [ 32 ], Bisha [ 33 ], and Aljouf [ 10 ]; with the majority being Escherichia coli (38%) accounting approximately for 52.9% of the gram-negative bacterial growth in line with previous national studies [ 7 , 29 , 30 , 32 , 34 ]. The second predominant isolates of the gram-negative organisms were the Klebsiella species (15.1%), this finding was similar to the bacterial isolates prevalence studies from Dhahran [ 7 ], and Riyadh [ 6 ].…”

Section: Discussionsupporting

confidence: 92%

Antimicrobial susceptibility of gram-positive and gram-negative bacteria: a 5-year retrospective analysis at a multi-hospital healthcare system in Saudi Arabia

Alhumaid

Mutair

Alawi

et al. 2021

Ann Clin Microbiol Antimicrob

View full text Add to dashboard Cite

Background Studying time-related changes in susceptible pathogens causing healthcare-associated infections (HAIs) is vital in improving local antimicrobial and infection control practices. Objectives Describe susceptibility patterns to several antimicrobials in gram-positive and gram-negative pathogens isolated from patients causing HAIs at three private tertiary care hospitals in Saudi Arabia over a 5-year period. Methods Data on trends of antimicrobial susceptibility among bacteria causing HAIs events in children and adults at three tertiary private hospitals located in Riyadh and Qassim, Saudi Arabia, were collected retrospectively between 2015 and 2019 using the surveillance data datasets. Results Over a 5-year period, 38,624 pathogens caused 17,539 HAI events in 17,566 patients. About 9450 (53.8%) of patients who suffered HAIs were females and the average age was 41.7 ± 14.3 years (78.1% were adults and 21.9% were children). Gram-negative pathogens were 2.3-times more likely to cause HAIs compared to gram-positive bacteria (71.9% vs. 28.1%). The ranking of causative pathogens in decreasing order was: Escherichia coli (38%), Klebsiella species (15.1%), and Staphylococcus aureus (12.6%). Gram-positive isolates were mostly susceptible to linezolid (91.8%) whereas they were resistant to ampicillin (52.6%), cefoxitin (54.2%), and doxycycline (55.9%). Gram-negative isolates were mostly sensitive to tigecycline (95%) whereas they were resistant to cefotaxime (49.5%) and cefixime (59.6%). During the 5 years, there were relatively stable susceptibility patterns to all tested antimicrobials, except for cefotaxime which shown a susceptibility reduction by 41.4%, among Escherichia coli and Klebsiella species. An increase in the susceptibility of Acinetobacter and Enterobacter and Citrobacter species to all studied antimicrobials was observed except for colistin that had a slight sensitivity reduction in 2019 by 4.3% against Acinetobacter species. However, we noted reduced sensitivity of MRSA, CoNS and Enterococcus species to gentamicin; and increased resistance of MRSA to linezolid and vancomycin. Conclusion The observed increase in susceptibility of gram-positive and gram-negative bacteria to studied antimicrobials is important; however, reduced sensitivity of MRSA, CoNS and Enterococcus species to gentamicin; and increased resistance of MRSA to linezolid and vancomycin is a serious threat and calls for effective antimicrobial stewardship programs.

show abstract

Section: Discussionsupporting

confidence: 92%

Antimicrobial susceptibility of gram-positive and gram-negative bacteria: a 5-year retrospective analysis at a multi-hospital healthcare system in Saudi Arabia

Alhumaid

Mutair

Alawi

et al. 2021

Ann Clin Microbiol Antimicrob

View full text Add to dashboard Cite

show abstract

“…Frequent pathogens responsible for UTI include Citrobacter , Enterobacter , Enterococcus , Escherichia , Klebsiella , Proteus , Pseudomonas , Serratia , and Staphylococcus , which colonise the genito-urinary tract. 6 , 8 , 9 , 10 , 11 Ahmed et al. 8 revealed that Klebsiella , Escherichia coli , Pseudomonas, and Proteus spp.…”

Section: Introductionmentioning

confidence: 99%

Rising prevalence of multidrug-resistant uropathogenic bacteria from urinary tract infections in pregnant women

Asmat

Mumtaz

Malik

2021

Journal of Taibah University Medical Sciences

View full text Add to dashboard Cite

Objectives This study aimed to determine the prevalence of urinary tract infections (UTI) in pregnant women and characterise the uropathogenic bacterial strains associated with symptomatic and asymptomatic bacteriuria in Lahore, Pakistan. Methods Between December 2018 and June 2019, we analysed the uropathogenic bacterial strains from midstream urine samples in 80 pregnant women. The age of the pregnant women ranged from 19 to 45 years, and they resided in urban and rural areas. We also recorded socioeconomic factors in this cohort. The isolated strains were phenotypically identified and evaluated for multiple drug resistance (MDR) patterns against recommended antimicrobial drugs. Results Of the 80 pregnant women, 65 had UTI, reflecting an 81% prevalence of UTI in women during pregnancy. The majority of participants aged 24–35 years, were multipara, and were in their third trimester. Results showed that 67 uropathogenic bacterial strains belonged to Escherichia (31%), Klebsiella (23%), Pseudomonas (16%), Streptococcus (4%), Enterococcus (4%), Staphylococcus (4%), and Proteus (3%) genera, as identified using biochemical characterisation. The highest overall resistance of Escherichia was seen against amoxicillin, pipemidic acid, and ampicillin; for Klebsiella against pipemidic acid, ampicillin, and cefotaxime; and for Pseudomonas against ciprofloxacin and cefotaxime. The three strains with the highest MDR were identified using 16S rRNA as Pseudomonas aeruginosa strain UA17, Escherichia coli strain UA32, and Klebsiella pneumoniae strain UA47. Conclusion In this study, the MDR uropathogenic strains showed the highest resistance pattern. The alarming signs of MDR uropathogenic infections are infrequently addressed and thus, urgent attention to this matter is essential.

show abstract

“…Another negative effect related to these devices is bacterial or fungal infections either caused by poor sterilization of the device or acquired in hospitals [4][5][6]. Innovative solutions of the last decade include the development of combination products, which are defined as a combination between a medical device and a drug and which allow for the targeted release of drugs at the surgical site [7,8].…”

Section: Introductionmentioning

confidence: 99%

Radiation Grafting of a Polymeric Prodrug onto Silicone Rubber for Potential Medical/Surgical Procedures

et al. 2020

View full text Add to dashboard Cite

Silicone rubber (SR) is a material used for medical procedures, with a common example of its application being in implants for cosmetic or plastic surgeries. It is also an essential component for the development of medical devices. SR was functionalized with the polymeric prodrug of poly(2-methacryloyloxy-benzoic acid) (poly(2MBA)) to render the analgesic anti-inflammatory drug salicylic acid by hydrolysis. The system was designed by functionalizing SR films (0.5 cm × 1 cm) with a direct grafting method, using gamma irradiation (60Co source) to induce the polymerization process. The absorbed dose (from 20 to 100 kGy) and the monomer concentration (between 0.4 and 1.5 M) were critical in controlling the surface and the bulk modifications of SR. Grafting poly(2MBA) onto SR (SR-g-2MBA) were characterized by attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy/energy-dispersive X-ray spectrometry, fluorescence microscopy, the contact angle, and the swelling. SR-g-2MBA demonstrated the drug’s sustained and pH-dependent release in simulated physiological mediums (pH = 5.5 and 7.4). The drug’s release was quantified by high-performance liquid chromatography and confirmed by gas chromatography–mass spectrometry. Finally, cytocompatibility was demonstrated in murine fibroblast and human cervical cancer cell lines. The developed systems provide new polymeric drug release systems for medical silicone applications.

show abstract

Prevalence of catheter associated biofilm producing bacteria and their antibiotic sensitivity pattern

Cited by 36 publications

References 32 publications

Antimicrobial susceptibility of gram-positive and gram-negative bacteria: a 5-year retrospective analysis at a multi-hospital healthcare system in Saudi Arabia

Antimicrobial susceptibility of gram-positive and gram-negative bacteria: a 5-year retrospective analysis at a multi-hospital healthcare system in Saudi Arabia

Rising prevalence of multidrug-resistant uropathogenic bacteria from urinary tract infections in pregnant women

Radiation Grafting of a Polymeric Prodrug onto Silicone Rubber for Potential Medical/Surgical Procedures

Contact Info

Product

Resources

About