Prevalence of Corynebacterial 16S rRNA Sequences in Patients with Bacterial and “Nonbacterial” Prostatitis

Tanner, Michael; Shoskes, Daniel A.; Shahed, Asha; Pace, Norman R.

doi:10.1128/jcm.37.6.1863-1870.1999

Cited by 144 publications

(41 citation statements)

References 38 publications

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“…Staphylococcus epidermis is of particular concern. An organism that was once dismissed as a laboratory contaminant is now considered an opportunistic pathogen that can cause bacteraemia, mediastinitis, meningitis and endocarditis in patients with prosthetic devices, and general infections in immunocompromised patients (Pfaller and Herwaldt 1988; Tanner et al. 1999).…”

Section: Discussionmentioning

confidence: 99%

Molecular survey of aeroplane bacterial contamination

McManus

Kelley

2005

J Appl Microbiol

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Aims: To examine bacterial contamination of passenger aircraft and to identify aeroplane environments posing the greatest potential health risk. Methods and Results: DNA was extracted from ten environmental samples collected on four different flights (three domestic, one international) from a variety of surfaces frequently touched by passengers. PCR clone libraries were made from the DNA samples using bacterial-specific 16S ribosomal DNA (rDNA) primers. A total of 271 bacterial rDNA sequences were obtained. We used BLAST analysis of the rDNA clone sequences to identify sequences in Genbank with the highest sequence similarity. The majority of the rDNA clones obtained from aeroplane environments were more than 97% identical to rDNA sequences from cultured bacterial species. Samples collected from the cabin surfaces (e.g., tray tables and arm rests) had undetectable levels of DNA and produced no PCR products. Bacterial diversity was highest on lavatory surfaces, including door handles, toilet handles, and sink faucets. Sequence data from these surfaces detected species from 58 different bacterial genera, and many of the best BLAST hits matched rDNA sequences of cultured species known to be opportunistic pathogens. The most frequently observed species came from five genera commonly associated with humans: Streptococcus, Staphylococcus, Cornybacterium, Proprionibacterium and Kocuria. Conclusions: The results show that there is a large diversity of bacterial contamination on aeroplanes, including organisms known to be opportunistic pathogens. Significance and Impact of the Study: Our results indicate that aeroplanes have the potential to spread an enormous diversity of bacterial species among passengers and destinations. Aeroplane lavatories present an especially significant concern to public health.

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Section: Discussionmentioning

confidence: 99%

Molecular survey of aeroplane bacterial contamination

McManus

Kelley

2005

J Appl Microbiol

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“…We have previously shown that the treatment and eradication of these bacteria leads to a resolution of symptoms in about a third of patients with CPPS . Furthermore, we have found diverse and unique Gram positive bacterial species in EPS of men with CPPS by 16S recombinant RNA techniques (Tanner et al, 1999). These observations suggest that these bacteria may be pathogens, or at least, markers of a unique subset of patients with CPPS.…”

Section: Discussionmentioning

confidence: 66%

“…It is clear that many men with category III CPPS respond to antimicrobial-based therapy, which may be due to undetected bacteria. In fact, molecular studies suggest a wide variety of organisms are present in EPS from these men (Tanner et al, 1999). What defines infection as opposed to colonization is the presence of tissue injury caused by bacteria and a documented injury response of the body's defenses.…”

mentioning

confidence: 99%

Oxidative Stress in Prostatic Fluid of Patients With Chronic Pelvic Pain Syndrome: Correlation With Gram Positive Bacterial Growth and Treatment Response

SHAHED,

SHOSKES

2000

Journal of Andrology

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The etiology of chronic pelvic pain syndrome (CPPS)/chronic prostatitis category III remains unknown. Whereas a subset of men respond to antimicrobial therapy, Gram positive bacteria isolated from expressed prostatic secretions (EPS) are often considered to be commensal rather than pathogenic. We wished to study oxidative stress as a marker of tissue injury and response in EPS of men with CPPS to determine whether infection with Gram positive bacteria is associated with increased oxidative stress. A total of 300 EPS specimens from 100 men with CPPS were collected for microscopy, culture, and biochemical and molecular assays. Oxidant injury was measured by 8‐isoprostane F2α (IsoP) levels and total antioxidant capacity as Trolox equivalents. Total RNA from EPS was used for gene expression of heme oxygenase‐1 (HO‐1) and granzyme B. The only bacteria found in EPS were Gram positive. For our analysis, these men were classified as having chronic bacterial prostatitis (category II). IsoP levels (pg/mL) were highest in men with category II prostatitis (7315 ± 1428) followed by nonbacterial prostatitis (category IIIa, 2043 ± 561), prostatodynia (category IIIb, 319 ± 81), and asymptomatic controls (298 ± 99). IsoP levels decreased significantly after successful treatment with antibiotics or an antioxidant supplement (Prosta‐Q). Antioxidant capacity was detected in 11 out of 18, 4 out of 16, and 1 out of 16 men tested with category II, IIIa, and IIIb prostatitis, respectively. No correlation was observed between IsoP levels and the number of white blood cells in EPS. HO‐1 and granzyme B expression was highest in men with category II prostatitis than in men with either category III prostatitis or asymptomatic controls. On the basis of elevated oxidative stress, clinical response to antibiotics, and post‐treatment reduction in oxidative stress, we conclude that Gram positive bacteria in some men with CPPS may be pathogens. It is speculated that oxidative stress may be a key pathway in some men with CPPS that can be targeted with antioxidant therapy.

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“…Preliminary biochemical tests showed the Gram‐negative bacterium to be both catalase‐ and oxidase‐positive, liquefied gelatin and utilized glucose oxidative in Hugh–Leifson’s (O/F) test. The identity of the organism was confirmed by sequencing PCR analysis of the 16S rDNA according to the method of Tanner et al. (1999) as previously described (Obuekwe et al.…”

Section: Methodsmentioning

confidence: 99%

Comparative hydrocarbon utilization by hydrophobic and hydrophilic variants ofPseudomonas aeruginosa

Obuekwe

Al-Jadi

Al-Saleh

2008

Journal of Applied Microbiology

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Aims: To investigate hydrocarbon degradation by hydrophobic, hydrophilic and parental strains of Pseudomonas aeruginosa. Methods and Results: Partitioning of hydrocarbon‐degrading P. aeruginosa strain in a solvent/aqueous system yielded hydrophobic and hydrophilic fractions. Exhaustive partitioning of aqueous‐phase cells yielded the hydrophilic variants (L), while sequential fractionation of the hydrophobic phase cells yielded successive fractions exhibiting increasing cell‐surface hydrophobicity (CSH). In hydrocarbon adherence assays (bacterial attachment to hydrocarbon), L had a value of 20%, which increased from 61·7% in first hydrophobic fraction (H1) to 72·2% in the third (H3). Crude oil degradation by L was 70%, but increased from 82% in H1 to 93% in H3. L variant produced most exopolysaccharides and reduced surface tension from about 73 to 49 mN m−1. Rhamnolipid production was highest in L, but was not detected in all crude oil cultures. Conclusions: Hydrophobic subpopulations of hydrocarbon‐degrading P. aeruginosa exhibited greater hydrocarbon‐utilizing ability than hydrophilic ones, or the parental strain. Significance and Impact of the Study: Results demonstrate that a population of P. aeruginosa consists of cells with different CSH which affect hydrocarbon utilization. This potentially provides the population with the capacity to utilize different hydrophobic substrates found in petroleum. Judicious selection of such hydrophobic subpopulations can enhance hydrocarbon pollution bioremediation.

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Prevalence of Corynebacterial 16S rRNA Sequences in Patients with Bacterial and “Nonbacterial” Prostatitis

Cited by 144 publications

References 38 publications

Molecular survey of aeroplane bacterial contamination

Molecular survey of aeroplane bacterial contamination

Oxidative Stress in Prostatic Fluid of Patients With Chronic Pelvic Pain Syndrome: Correlation With Gram Positive Bacterial Growth and Treatment Response

Comparative hydrocarbon utilization by hydrophobic and hydrophilic variants ofPseudomonas aeruginosa

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