Prevalence of Eimeria species in scavenging native chickens of Shiraz, Iran

Hadipour, Mohammadmehdi; Olyaie, A.; Naderi, Mohammad; Azad, F.; Nekouie, Omid

doi:10.5897/ajmr11.477

Cited by 31 publications

(6 citation statements)

References 18 publications

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“…The shape (length/width) of the sporulated oocysts were determined by using the method for species identification as described by Hadipour et al (11). The calculated oocysts shape index values were then compared with the standard diagnostic guide provided by Teixeira and Lopes (12) to determine the species encountered in the study.…”

Section: Sporulation Of Parasitementioning

confidence: 99%

Phynotypic and genotypic identification of Eimeria species in backyard chicken in Nineveh governorate, Iraq

Ahmed¹,

Al-Bakri²

2021

IJVS

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Coccidiosis is an intestinal disease caused by a parasite of the genus Eimeria. This parasite mainly affects poultry species and causes great economic losses in the poultry industry. This study was designed to estimate the prevalence of coccidiosis in the local breed of domestic chicken in Nineveh Governorate, Iraq. 450 faecal swabs and intestinal samples (intestinal scraping) were collected from different local breeds of home-bred chickens from October 2020 to the end of March 2021. All fecal samples were examined using the flotation method by using sugar solution, and Eimeria was confirmed by the polymerase chain reaction method. Fecal examination results showed that 32.6% of the total samples were positive for Eimeria oocysts, classified into six species including E. brunetti, E. mitis.E. maxima E. acervulina E. necatrix, E. tenella with infection rates are 57.5, 44.2, 42.1, 26.5, 20.4, 16.3%, respectively. The phenotypic results were genetically confirmed by the result of the reaction of 455 base pairs. The prevalence of coccidiosis was highest in chickens less than three months of age 49.2% and lowest in chickens older than 6 months 23.9%.

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Section: Sporulation Of Parasitementioning

confidence: 99%

Phynotypic and genotypic identification of Eimeria species in backyard chicken in Nineveh governorate, Iraq

Ahmed¹,

Al-Bakri²

2021

IJVS

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“…Anticoccidial drugs in birds have resulted in drug resistance as well as the presence of drug residues in chicken products like meat and eggs, ultimately posing a major public health risk 12 . However, the unavailability of new anticoccidial drugs demands novel anticoccidial alternatives 13 . Nanotechnology methods have currently been incorporated into the veterinary industry, not only for disease diagnosis but also for drug development and prevention of diseases 14 .…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis and characterization of zinc oxide nanoparticles using Nigella sativa against coccidiosis in commercial poultry

Lail

Sattar

Omer

et al. 2023

Sci Rep

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Coccidiosis causes huge economic losses worldwide. Current study evaluated the effect of biosynthesized Zinc oxide nanoparticles (ZnONPs) using Nigella sativa, on Eimeria tenella infected broilers. Scanning electron microscopy showed spherical ZnONPs with 50–100 nm diameter, Fourier transforms infrared spectroscopy revealed the functional groups involved in the reduction of zinc acetate dihydrate to ZnONPs, UV–vis spectroscopy showed a peak at 354 nm, and Zeta potential exhibited stability at − 30 mV. A total of 150, a day-old broiler chicks were divided into 5 equal groups. Control negative: uninfected and untreated; Control positive: Infected and untreated; 3rd, 4th and 5th group were infected orally with 5 × 104 sporulated oocysts of Eimeria tenella and treated with 60 mg/kg ZnONPs, 1% Nigella sativa seeds and amprolium 125 ppm, respectively. ZnONPs significantly (p < 0.05) improved the growth performance in the infected birds and decreased the oocyst shedding and anti-coccidial index. A significant (p < 0.05) decrease in the level of aspartate transferase and alanine transferase, whereas, a significantly higher amount of antioxidants like catalase and superoxide dismutase in ZnONPs treated group was observed. Pro-inflammatory cytokines like IL-2 and TNF-α were significantly decreased by ZnONPs (p < 0.05). In conclusion, biogenic ZnONPs with Nigella sativa might have enhanced anticoccidial, antioxidant, and anti-inflammatory effects with improved growth performance.

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“…Conventionally, traditional methods for Eimeria species identification are based on morphology and/or morphometry of the sporulated oocyst, pre‐patent period, sporulation time, and site of pathological lesions due to Eimeria infection (Aarthi et al, 2010; Hadipour et al, 2011). Identification through these methods sometimes lacks accuracy due to overlapping characteristics and mixed infections among different Eimeria species, which leads to a problem for precise Eimeria species discrimination (Carvalho et al, 2011; Meireles et al, 2004; Morgan et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Identification of Eimeria acervulina (Apicomplexa: Eimeriidae) infecting the broiler chicken Gallus gallus domesticus through morphology and molecular analyses

Mohammed,

Al‐Shaebi,

Al‐Quraishy

et al. 2023

Animal Physiology Nutrition

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Coccidiosis is an intestinal protozoan disease that affects the poultry industry worldwide. The severity of this disease varies depending on the identity of the infectious agents. Therefore, this study was carried out to identify the Eimeria species that affect broiler chickens, Gallus gallus domesticus, through morphological and molecular phylogenetic analyses. Twenty‐five faecal samples were collected from the broiler chickens in a commercial poultry farm in Riyadh (Saudi Arabia). Using the floatation technique, faeces were examined microscopically for the Eimeria occurrence. Identification of Eimeria species was performed based on morphological criteria and molecular tools (DNA amplification for the partial small subunit ribosomal RNA (18S rRNA), internal transcribed spacer (ITS)‐1, and mitochondrial cytochrome c oxidase I (COI) genes. In this study, 32% (8 out of 25) of collected samples were found to be positive for coccidiosis. After sporulation in potassium dichromate (K2Cr2O7), the sporulated oocysts were observed as ovoid and measured 18.37–23.19 µm (19.87) long and 15.07–18.67 µm (16.46) wide, with the anterior location of a polar granule and absence of micropyle. These Eimeria oocysts were assumed to size and shape characteristics of Eimeria acervulina. Molecular analysis was conducted on the sequences of the polymerase chain reaction products from the three genes studied (18S rRNA, ITS‐1, and COI). At the three genes, results showed that the resultant sequences clustered with E. acervulina from different regions confirming morphological description. This study highlighted the importance of molecular techniques to detect avian Eimeria species more than the traditional morphology‐based tool to optimise the appropriate anticoccidial strategies for long‐term control in the studied area.

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Prevalence of Eimeria species in scavenging native chickens of Shiraz, Iran

Cited by 31 publications

References 18 publications

Phynotypic and genotypic identification of Eimeria species in backyard chicken in Nineveh governorate, Iraq

Phynotypic and genotypic identification of Eimeria species in backyard chicken in Nineveh governorate, Iraq

Biosynthesis and characterization of zinc oxide nanoparticles using Nigella sativa against coccidiosis in commercial poultry

Identification of Eimeria acervulina (Apicomplexa: Eimeriidae) infecting the broiler chicken Gallus gallus domesticus through morphology and molecular analyses

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