Prevalence of enterococci and its antibiotic resistance in various clinical samples at tertiary care hospital in Southern Rajasthan, India

Atray, Deepika; Sharma, Anshu; Atray, Meena

doi:10.18203/2320-6012.ijrms20162303

Cited by 8 publications

(10 citation statements)

References 7 publications

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“…Enterococcus was the predominant microorganism isolated from these samples (43.75%). This was comparable to the findings of Atray D et al 6 Chakraborty et al, conducted a study in Kolkata which also showed enterococcus was the predominant organism (66%) isolated from urine samples. 7 In this study, the other common pathogens isolated were E. coli (28.45%) and Klebsiella (14.89%).…”

Section: Discussionsupporting

confidence: 89%

Antibiotic sensitivity pattern of bacterial isolates from urine samples of admitted patients with urinary tract infection in a tertiary care teaching hospital of Tripura, India: a hospital record based study

Das

Bhattacharjee

Debnath

et al. 2018

Int J Basic Clin Pharmacol

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Background: Urinary tract infection (UTI) being one of the most common and a serious health problem both in the community and hospital settings each year worldwide, the emergence of antibiotic resistance in the management of UTI is a serious public health issue. The present study will analyse the antimicrobial sensitivity pattern of pathogens isolated from the urine samples of admitted patients suffering from UTI in Tripura Medical College and Dr. B.R. Ambedkar Memorial Teaching Hospital (TMC).Methods: This was a hospital record-based study. The urine samples of clinically diagnosed UTI patients admitted in various departments of the hospital during the study period were included. The reports of culture and sensitivity testing of the samples were collected. The results were interpreted according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI).Results: During the 12-month study period, a total of 752 urine samples were analysed. Enterococcus (43.75%) was the most frequently isolated bacteria, followed by E. coli (28.45%) and Klebsiella (14.89%). Enterococcus was highly sensitive (p<0.001) to vancomycin (95.33%), E. coli was mostly sensitive to nitrofurantoin (83.65%) and Klebsiella mainly sensitive to imipenem (75.49%).Conclusions: The study showed that positive urine culture with the antibiotic sensitivity of the isolates is very important for antimicrobial therapy, as antibiotic resistance is a worldwide problem which causes ineffectiveness of treatment.

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Section: Discussionsupporting

confidence: 89%

Antibiotic sensitivity pattern of bacterial isolates from urine samples of admitted patients with urinary tract infection in a tertiary care teaching hospital of Tripura, India: a hospital record based study

Das

Bhattacharjee

Debnath

et al. 2018

Int J Basic Clin Pharmacol

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“…This is possibly due to the contamination from human handlers [ 18 ], as strains with rare and high antibiogram patterns were generally isolated from hospital settings because of a high antimicrobial pressure [ 54 ]. Earlier, vancomycin-resistant enterococci strains were isolated from tertiary care hospitals in Rajasthan [ 55 , 56 ], and it was proved that the gene cluster VanA was transferable from E. faecalis to S. aureus [ 57 ]. Similarly, teicoplanin-resistant S. aureus strains were reported from a hospital in Jaipur, Rajasthan [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Coagulase gene polymorphism, enterotoxigenecity, biofilm production, and antibiotic resistance in Staphylococcus aureus isolated from bovine raw milk in North West India

Sharma

Dahiya

et al. 2017

Ann Clin Microbiol Antimicrob

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Background Staphylococcus aureus is the predominant bacterium responsible for various diseases in animals and humans. Preventive strategies could be better implemented by understanding the prevalence, genetic patterns, and the presence of enterotoxin and biofilm-producing genes along with the antibiotic susceptibility of this organism. This study was conducted in Rajasthan, the northwestern state of India, holding the largest population of cattle that makes it the second largest milk producer in India and no such prior information is available on these aspects.MethodsA total of 368 individual quarter bovine raw milk samples were collected from 13 districts of Rajasthan, and screened for the presence of S. aureus. Microbiological and molecular approaches were followed for bacterial identification. Genetic diversity was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) of coagulase gene (coa), whereas enterotoxin and biofilm-producing genes were studied by PCR analysis. Antibiotic strips were employed to study the antibiotic resistance among strains.ResultsIn all, 73 S. aureus strains were obtained from 368 bovine raw milk samples out of that only 30 showed the presence of coa. Nine types of coa patterns ranging from 730 to 1130 bp were observed among these isolates. PCR–RFLP of coa distinguished the isolates into 15 genotypic patterns, of which patterns I, IV, V, and VI were predominant. Of the isolates, 30% were positive for sec, 10% for sea, and 3.3% for seb; these genes are responsible for enterotoxin production, whereas all isolates were found positive for icaAD and eno. The prevalence rates of other biofilm-producing genes fnbA, clfB, ebpS, sasG, fnbB, sasC, cna, bap, fib and, bbp were 97, 93, 90, 80, 80, 77, 53, 27, 10, and 6.6%, respectively. Twenty-seven (90%) strains were multidrug resistant, of which 15 were methicillin resistant. Maximum sensitivity was reported for kanamycin and it could be considered as a drug of choice for controlling S. aureus mediated cattle infections in the studied regions.ConclusionsOverall, these strains could cause several diseases to humans, insisting the need for developing a stricter hygiene program for improving milking practices and animal health.

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“…Multiple organisms are presence in urinary tract through which urine flows from kidney viathe bladder. The urinary tractinfectionis the second most common type of infection [1]. Urinary tract infections are mostly caused by gramnegative bacteria like, E.coli, Klebsiella species, Proteus mirabilis, Pseudomonas aeruginosa, Acinetobacter, and Serratia.…”

Section: Introductionmentioning

confidence: 99%

“…[8].Klebsiella pneumoniae is an opportunistic pathogens responsible for a wide spectrum of hospital communityacquired and nosocomial infection especially patients suffering from indwelling devices of medicalsuch ascatheters [9].K.pneumoniae possessing two types of fimbriae or piliwhich are nonflagellar, and showed mainly on the basis of ability of agglutinating erythrocytes ofspecies of different animal [7]. Type 3 fimbriae have showedof mediating the initiation of biofilmsproducing on biotic and abiotic surfaces, in addition of being required formature biofilms formation [10,11].The mannose-resistant type 3 pili (T3P or MR/K), constituent of the major pilussubunit mrkA and the minor tip adhesin mrkD [12].Biofilms plays a main role in virulence of bacteria [1]. The possible relationship between bacteria persistence in the urinary tract andthe presence of virulence factors (VFs) lead to biofilmsproduction like adhesins, toxins, lipopolysaccharides, iron acquisition, presence of capsule and serum resistance [1,13].…”

Section: Introductionmentioning

confidence: 99%

“…Type 3 fimbriae have showedof mediating the initiation of biofilmsproducing on biotic and abiotic surfaces, in addition of being required formature biofilms formation [10,11].The mannose-resistant type 3 pili (T3P or MR/K), constituent of the major pilussubunit mrkA and the minor tip adhesin mrkD [12].Biofilms plays a main role in virulence of bacteria [1]. The possible relationship between bacteria persistence in the urinary tract andthe presence of virulence factors (VFs) lead to biofilmsproduction like adhesins, toxins, lipopolysaccharides, iron acquisition, presence of capsule and serum resistance [1,13]. Biofilms formation capable singlecellorganisms to assume a temporary multicellular lifestyle, in that organisms behavior facilitates survival in adverse environments [13].…”

Section: Introductionmentioning

confidence: 99%

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DetectionOf genesResponsible for BiofilmsFormedby Klebsiella pneumoniaeandEsherichia coliand their effect on innate immunity

Alwan

Khwen

2018

AJPS

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Biofilms as a major virulence factor of bacteria ,therelationship between bacteria persistence in the urinary tract and genes responsible for biofilms production was studied. The aim is to detect the presence,frequency of the,fimA, fimH, mrkA and mrkD, genes and biofilms production in two species isolated from UTI patients and their effect on some innate immunity aspects. Sixty five isolates of E.coli and fifty of K.pneumoniae isolates were collected. All isolates were initially diagnosed as genus Esherichiacoli andKlebsiella pneumonia. Final identification for the isolates was done by Vitek 2 compact system. The ability to form biofilm was carried out by using Tissue culture plate method (TCP).Adhesion average to epithelial cells of E.coli and K.pneumoniae was studied. Detection of genes were done to by using PCR technique . Immunological experiments were done by using bactericidal activity and opsonization factor. UTI infection were more common in female than male. 75% (49/65) females, 25%(16/65) males ofE.coli isolates and 88% (44/50) females,12%(6/50) males of K.pneumoniaeisolates. Patients ages in children between (2 months -18years) in UTI caused by E.coli was 35%(23/65) while in k.pneumoniae was 74%(37/50),Ages between (18 years-50 years) in E.coli was 37%(24/65) while in K.pneumoniae was 18%(9/50) finally,Ages between (50 years-72years) in E.coli was 28%(18/65) while in K.pneumoniae was 8%(4/50).69% of E.coli isolates and 72% of k.pneumoniae isolates were higher biofilm producers. . Adhesion average to epithial cells of E.coli and K.pneumoniae was 42.9% and 44% respectively. Both E.coli and K.pneumoniae showed that 100% 0f the 12 isolate harbored the fimA, fimH, mrkA and mrkD genes. The bactericidal average was higher in E.coli than K.pneumoniae , P- value( 0.01) for mice injected with fimbriae, Opsonization factor was higher in E.coli than K.pneumoniae the P value (0.05) . Conclusion: A strong relationship between biofilms production and presence of genes of different species isolated from same clinical source that has effected on resistance of bacteria to innate immunity.

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Prevalence of enterococci and its antibiotic resistance in various clinical samples at tertiary care hospital in Southern Rajasthan, India

Cited by 8 publications

References 7 publications

Antibiotic sensitivity pattern of bacterial isolates from urine samples of admitted patients with urinary tract infection in a tertiary care teaching hospital of Tripura, India: a hospital record based study

Antibiotic sensitivity pattern of bacterial isolates from urine samples of admitted patients with urinary tract infection in a tertiary care teaching hospital of Tripura, India: a hospital record based study

Coagulase gene polymorphism, enterotoxigenecity, biofilm production, and antibiotic resistance in Staphylococcus aureus isolated from bovine raw milk in North West India

DetectionOf genesResponsible for BiofilmsFormedby Klebsiella pneumoniaeandEsherichia coliand their effect on innate immunity

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