Prevalence of Extended Spectrum β-Lactamase, AmpC β-Lactamase and Metallo-β-Lactamase Among Gram Negative Bacilli Recovered From Clinical Specimens in Benin City, Nigeria

Ibadin, Ephraim Ehidiamen; Omoregie, Richard; Anogie, Nana Atinuke; Igbarumah, Isaac Ohiorenuan; Ogefere, Helen Oroboghae

doi:10.15171/ijep.2017.20

Cited by 17 publications

(24 citation statements)

References 16 publications

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“…Though, the coexistence of ESBL with other types of β-lactamases is no morea rare event, only one isolate co-harbored ESBL and MBL in this study (Table 3). A similar concomitant presence of 2 β-Lactamases in one strain has been reported by Ibadin et al [20] in Southwestern Nigeria. The co-existence of ESBL and MBL enzymes in a bacterium will severely limit treatment options in treating infections caused by the strain [19][20][21].…”

Section: Resultssupporting

confidence: 86%

“…A similar concomitant presence of 2 β-Lactamases in one strain has been reported by Ibadin et al [20] in Southwestern Nigeria. The co-existence of ESBL and MBL enzymes in a bacterium will severely limit treatment options in treating infections caused by the strain [19][20][21]. It should be noted that these enzymes are hardly screened for, during routine laboratory susceptibility testing of clinical bacterial isolates in healthcare facilities in Nigeria.…”

Section: Resultssupporting

confidence: 86%

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First phenotypic and molecular characterization of clinical isolates of drug resistant Providencia rettgeri in Imo state, South-Eastern Nigeria.

Ugwu¹,

Ndunagu²,

Ejikwugwu³

et al. 2020

Asian J Biomed Pharmaceut Sci

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Background: Nosocomial outbreak of drug resistant Enterobacteriaceae including those mediated by Providencia species has become a serious public health challenge across the globe-owing to the difficulty in selecting antibiotics for proper treatment especially in the face of resistance. The morbidity and mortality rate of bacteremia due to Providencia species could be high, especially among the elderly and those with pre-existing medical conditions. This study was aimed at determining the occurrence of multidrug resistance phenotypes in clinical isolates of Providencia rettgeri using both phenotypic and genotypic techniques. Methods: Clinical isolates were identified to be isolates of P. rettgeri using standard microbiological and biochemical techniques; and these were characterized with respect to antibiotic resistant patterns. Overall, 143 clinical isolates of non-duplicate P. rettgeri isolates were bacteriologically recovered from the clinical specimens which included blood/wound swab samples (n=108) and swabs from surgical instruments and suction devices (n=35). Antimicrobial susceptibility test (AST) was done using the modified Kirby-Bauer disk diffusion techniques as per the Clinical Laboratory Standard Institute (CLSI) criteria. The isolates were tested phenotypically for ESBL production by the double disk synergy test (DDST) method and the β-lactamase genes were identified using polymerase chain reaction. The strains were sequenced with ABI3500XL analyzer with a 50 cm array, using POP7. Sequences data generated were analyzed with Geneious version 9. 0. 5. Results: The result of AST showed that Providencia rettgeri isolates were resistant to ceftazidime, cefotaxime, ampicillin and tetracycline. Based on the DDST method, some of the Providencia rettgeri isolates were phenotypically confirmed to be ESBL positive (11%) and MBL positive (6%). The results of the molecular analysis showed that the P. rettgeri isolates harbored SHV genes (n=6; 12. 5%) which mediates ESBL production. However, none of the isolates harboured the CTX-M or TEM gene. Conclusion: Our study shows that the P. rettgeri isolates analyzed were multidrug resistant, and they also harbour some important phenotypes and/or genotypes that mediate the production of ESBL (extended spectrum beta-lactamase). This is the first report of ESBL detection in clinical isolates of P. rettgeri in Imo state, southeast Nigeria.

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Section: Resultssupporting

confidence: 86%

Section: Resultssupporting

confidence: 86%

First phenotypic and molecular characterization of clinical isolates of drug resistant Providencia rettgeri in Imo state, South-Eastern Nigeria.

Ugwu¹,

Ndunagu²,

Ejikwugwu³

et al. 2020

Asian J Biomed Pharmaceut Sci

View full text Add to dashboard Cite

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“…Also, cefuroxime, cefixime and cefotaxime showed poor activity especially to Gram negative bacterial isolates. The presence of extended spectrum β-lactamase-and AmpC β-lactamase-producing bacteria has been recently demonstrated among clinical specimens including sputum (33.9% and 9.7% respectively) in Benin, Nigeria (19). This may have played roles in the high level of resistance observed to these cephalosporins.…”

Section: Discussionmentioning

confidence: 97%

“…This may have played roles in the high level of resistance observed to these cephalosporins. Also, antibiotic abuse is rife in our setting and over-the-counter purchase is rampant (3,19). Practices like this may create selective pressure and ensure the survival of resistant bacterial strains in the hospital and community settings.…”

Section: Discussionmentioning

confidence: 99%

Etiologic agents of lower respiratory tract infections among patients attending tuberculosis clinic in Benin City, Nigeria

Ibadin¹,

Ogefere²,

Ehondor³

2018

Af J Clin Exp Micro

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The emergence of HIV has reawakened the tuberculosis (TB) scourge and infected patients are prone to opportunistic infections, this study was carried out to determine the HIV status and etiologic agents of Lower respiratory tract infections (LRTI) among tuberculosis suspected patients in Benin City, Nigeria. The study was cross sectional. A total of 276 patients attending tuberculosis clinic were recruited. Questionnaires were filled for each patient. Blood and sputum specimens were collected in plain and sterile containers respectively and transported immediately to the laboratory. Blood samples were screened for the presence of HIV antibodies. The sputum specimens were then cultured following standard microbiological procedure, and thereafter processed using the GeneXpert MTB/RIF assay. Emergent bacterial colonies were identified and susceptibility testing was carried out following standard microbiological techniques. A total of 118 (42.8%) non-mycobacterial bacterial agents were recovered from the patients. Patients were more likely to be culture positive for LRTI in comparison with TB infection (p < 0.0001). There was no association between TB and HIV status (OR = 0.6161, 95%CI = 0.226, 1.648, p = 0.4474). HIV positive patients were more likely to be culture positive for bacterial agents and Klebsiella pneumoniae was more likely to be recovered (p = 0.0338). The fluoroquinolones, gentamicin and ceftriaxone-sulbactam were the most active antibacterial agents against bacterial isolates. The prevalence of LRTI in this study was 52.2%. The study draws attention on the need for physicians to request for bacteriological culture (non-mycobacterial) alongside the TB diagnostic algorithm in suspected TB cases.

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“…22 In the present study, Klebsiella spp was found to be the most common ESBL producer which was in concordance with the previous studies of Nepal and other countries. [23][24][25][26] who had also reported that Klebsiella spp were the predominant ESBL producers among the clinical isolates. However, another study from Nepal has reported that Escherichia coli were the predominant ESBL producers among the clinical isolates.…”

Section: Original Research Articlementioning

confidence: 99%

Prevalence of Extended Spectrum Beta Lactamase Producing and Carbapenems Resistance Isolates from Hands of Health Care Workers in a Health Care Setting of Nepal

Sharma

2020

J. Gandaki Med. Coll. Nepal

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Background: Hands of healthcare workers (HCWs) could be colonized by potential drug resistant bacteria like Extended Spectrum Beta Lactamase producers (ESBLs) and Carbapenems-resistant (CR) isolates and could become vectors of nosocomial pathogens in healthcare facilities that are associated with an increase of morbidity, mortality and healthcare costs. This study aimed to investigate the prevalence of ESBLs and CR isolates from hands of HCWs with their antibiotic susceptibility pattern. Materials and methods: This was a cross-sectional study that included a total of 150 hand swabs collected from March, 2018 to September, 2018 in Gandaki Medical College and Teaching Hospital. Isolation, identification and antimicrobial susceptibility tests were done using standard microbiological procedures. Results: Among the total isolates of 219 obtained from growth positive samples 92/219(42.01%) were Gram negative bacteria (GNB) and the most common were Klebsiella spp 32(34.78%) followed by Escherichia coli 17(18.48%), Pseudomonas aeruginosa12 (13.04%), Acenetobacter spp 11(11.96%), Proteus spp 9(9.78%), Citrobacter spp 7(7.61%) and Enterobacter spp 4(4.35%). The prevalence of ESBLs, CR and ESBLs with Co-resistant to Carbapenems were 19.56%,14.13% and 9.78%, respectively. The most effective drugs for isolates were Nitrofurantoin followed by Amikacin, Tetracycline and Gentamycin. Distribution pattern of the ESBLs and CR isolates among doctors, nurses, laboratory technicians, helpers and basic science faculties were not significant (p>0.05). Conclusions: This report revealed the emerging and moderately high prevalence of ESBLs, CR and ESBLs with Co-resistant to Carbapenems GNB with their antibiotic susceptibility patterns found on hands of HCWs in Nepal. Thus, this study could be helpful in developing proper guidelines on hand hygiene and implementation of infection control measures including contact precautions against the spread of infections by such pathogens in healthcare settings.

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Prevalence of Extended Spectrum β-Lactamase, AmpC β-Lactamase and Metallo-β-Lactamase Among Gram Negative Bacilli Recovered From Clinical Specimens in Benin City, Nigeria

Cited by 17 publications

References 16 publications

First phenotypic and molecular characterization of clinical isolates of drug resistant Providencia rettgeri in Imo state, South-Eastern Nigeria.

First phenotypic and molecular characterization of clinical isolates of drug resistant Providencia rettgeri in Imo state, South-Eastern Nigeria.

Etiologic agents of lower respiratory tract infections among patients attending tuberculosis clinic in Benin City, Nigeria

Prevalence of Extended Spectrum Beta Lactamase Producing and Carbapenems Resistance Isolates from Hands of Health Care Workers in a Health Care Setting of Nepal

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