Prevalence of extended-spectrum β-lactamase-producing Enterobacteriaceae in meat products sold in Navarra, Spain

Ojer-Usoz, Elena; González, David Herrera; Vitas, Ana Isabel; Leiva, José; García-Jalón, Isabel; Febles-Casquero, Alejandro; Escolano, María de la Soledad

doi:10.1016/j.meatsci.2012.09.009

Cited by 68 publications

(79 citation statements)

References 46 publications

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“…Several factors, such as human and animal antibiotic overuse, human cross-infection, transmission from pets or other animals via the food chain, among others, have contributed to the dissemination outside hospitals (18,20,21). The high circulation of ESBL-producing E. coli in healthy children in our region should alert us to the possibility of an increase of these strains in infected patients.…”

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confidence: 91%

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High Rate of Fecal Carriage of Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Healthy Children in Gipuzkoa, Northern Spain

Fernández‐Reyes

Vicente

Gomariz

et al. 2014

Antimicrob Agents Chemother

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The prevalence of extended-spectrum-␤-lactamase-producing Enterobacteriaceae (ESBLPE) was studied in stool samples from 125 8-to 16-month-old healthy children. Twenty-four percent of them and 10.7% of the 318 fecal samples studied yielded extended-spectrum-␤-lactamase-producing Escherichia coli, with the types being SHV-12, CTX-M-1, CTX-M-14, and TEM-52, the most common types of ␤-lactamases. This high prevalence of ESBLPE in healthy people, which is to our knowledge the highest currently reported in Europe, may represent a risk for increased infections by these organisms in the future.

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confidence: 91%

“…CTX-M-1, CTX-M-14, and SHV-12 were the most prevalent ESBL-producing E. coli strains found in chickens and other animal foods in Spain (18,19), suggesting that foods could be the source of acquisition of these resistant isolates in children.…”

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confidence: 99%

High Rate of Fecal Carriage of Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Healthy Children in Gipuzkoa, Northern Spain

Fernández‐Reyes

Vicente

Gomariz

et al. 2014

Antimicrob Agents Chemother

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“…These data concerns more often the occurrence of the mentioned microorganisms in animals at the farm (3, 4) or the slaughter-house (5, 9), and in less or more processed meat or meat products (8,20,25). The investigations concerning raw meat were performed most often with poultry meat (2,6,28), and more rarely with meat of other animal species (23,32).…”

Section: Discussionmentioning

confidence: 99%

Occurrence of extended spectrum β-lactamaseand AmpC-producing Escherichia coli in meat samples

Wasiński

Różańska

Osek

2013

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was a preliminary determination of occurrence of extended spectrum β-lactamases (ESBL)-and AmpC-producing Escherichia coli (E.coli) in raw meat samples collected from slaughter-houses located in different regions of Poland. A total of 141 samples were tested, comprising 78 pork samples, 44 beef samples, and 19 chicken meat samples. Isolated and identified E. coli strains were examined for the ESBL and/or AmpC β-lactamases production by the use of four disc diffusion and minimum inhibitory concentration tests. All strains positive in one or both tests were examined by PCR for the presence of the bla CTX , bla TEM , bla SHV , and bla CMY-2 group genes. During the study, 154 E. coli strains were isolated from 95 samples. Among these, 18 (11.7%) strains were identified in phenotypic tests as ESBL-producing and seven (4.5%) strains as AmpC-positive. The presence of the genes encoding selected ESBL-s (TEM, CTX, SHV) was identified in 14 of the strains recognised as ESBLpositive in phenotypic tests. All AmpC-positive isolates showed the presence of the CMY-2 group encoding genes. One of these strains had also the CTX-M and TEM genes, and four of them expressed the TEM marker.

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“…A positive association has been found between an increase in antimicrobial usage in veterinary medicine and the increasing prevalence of antimicrobial resistant bacteria detected in foodproducing animals and food products (Altalhi et al, 2010;Borjesson et al, 2013;Castellanos et al, 2017;da Costa et al, 2010;Hammerum et al, 2012;Hasan et al, 2011;Hordijk et al, 2013;Jiang et al, 2011;Nilsson et al, 2014;Ojer-Usoz et al, 2013).…”

Section: Public Health Significance Of Apecmentioning

confidence: 99%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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Prevalence of extended-spectrum β-lactamase-producing Enterobacteriaceae in meat products sold in Navarra, Spain

Cited by 68 publications

References 46 publications

High Rate of Fecal Carriage of Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Healthy Children in Gipuzkoa, Northern Spain

High Rate of Fecal Carriage of Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Healthy Children in Gipuzkoa, Northern Spain

Occurrence of extended spectrum β-lactamaseand AmpC-producing Escherichia coli in meat samples

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

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