Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Food-Producing Animals

Abstract

ABSTRACT. To evaluate the diversity of extended-spectrum -lactamases (ESBL) genes among food-producing animals, 48 isolates of ESBL-producing Escherichia coli isolates were obtained from rectal samples of broilers, layers, beef cattle and pigs, at the slaughterhouse level. ESBL-carrying E. coli were isolated from 60.0% of individual broiler rectal samples, 5.9% of layers, 12.5% of beef cattle and 3% of pigs. One ESBL-producing Klebsiella pneumoniae was isolated from a broiler. The ESBL-positive E. coli isolates from broilers harbored various ESBL genes: bla , bla CTX-M-2 , bla CTX-M-14 , bla CTX-M-15 and bla CTX-M-44 . The plasmid DNAs were analyzed by restriction patterns. Homogeneous band patterns were yielded in those of K. pneumoniae and E. coli isolates harboring the bla CTX-M-2 gene from different farms. No genetic relation between the 2 CTX-M-14 ESBL-producing strains was found by pulsed-field gel electrophoresis, although 2 plasmids in these strains, obtained from different broiler farms, were similar to each other. This study provides evidence that the proliferation of CTX-M-producing E. coli is due to the growth of indigenous CTX-M-producing strains and the possible emergence of strains that acquired CTX-M genes by horizontal transfer in different broiler farms. CTX-M-producing coliforms in broilers should be controlled due to the critical importance of cephalosporins and the zoonotic potential of ESBL-producing bacteria.

Antimicrobial Resistance in ESBL-Producing Escherichia coli Isolated from Layer and Pig Farms in Thailand

“…This is at variance with the results of studies by Hiroi et al and Geser et al, in which layers are recognized as the major reservoir of ESBL-producing E. coli [14,15].…”

MOLECULAR CHARACTERIZATION AND IMMUNOPROTECTIVE ACTIVITY OF CAPSULAR POLYSACCHARIDE OF <I>KLEBSIELLA PNEUMONIAE</I> ISOLATED FROM FARM ANIMALS AT TAIF GOVERNORATE

“…As shown in Table 2 it was concluded that in diseased cows the highest rate of isolation was obtained from faecal samples (32.1%) followed by nasopharyngeal swabs (28.7%), the lowest incidence could be isolated from milk samples (16.0%). These results agreed to great extent with the finding of Hiroi et al (2012) who isolated Klebsiella pneumoniae from nasal swabs and bronchial mucous collected from calves suffering from bronchopneumonia. Moreover, Munoz et al (2007) reported that Klebsiella pneumoniae had been isolated from faecal samples of diarrhoeic calves.. As regard to sheep samples in this study Klebsiella pneumoniae was mainly predominant in faecal samples, nasopharyngeal swabs and milk samples 40.9, 17.4 and 14.3%, respectively.…”

Detection of <i>Escherichia coli</i> Producing CTX-M-1-Group Extended-Spectrum β-Lactamases from Pigs in Aichi Prefecture, Japan, between 2015 and 2016

“…Because the food chain could be a major route of bacterial transmission to humans, we speculated that food-producing animals might also act as reservoirs for these drug-resistant microorganisms in Japan. Some investigations have found that ESBL-producing E. coli have been increasingly isolated from chicken meat samples (7,8). However, epidemiological data describing ESBL-producing E. coli isolated from pigs remain quite limited in Japan.…”