Prevalence of extended spectrum β-lactamases (esbls) among enterobacteriaceae in murtala mohammed specialist hospital, Kano, Nigeria

Yusha’u, M; Aliyu, H. M.; Kumurya, Abdulhadi Sale; Suleiman, K M

doi:10.4314/bajopas.v3i1.58756

Cited by 39 publications

(46 citation statements)

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“…The results showed that 37 (9.25%) isolates were ESBL producers based on DDST while only 20 (5.00%) were positive using the NCCLS breakpoints (Yusha'u et al, 2007).…”

Section: Introductionmentioning

confidence: 98%

Prevalence of extended spectrum &#946-lactamases (ESBLs) among clinical <i>Enterobacteriaceae</i> isolates obtained from private diagnostic laboratory in Kano - Nigeria

Yusha’u¹,

Adakawa²,

Kawo³

2011

International Journal of Biological and Chemical Sciences

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One hundred Gram negative bacterial isolates were collected from a private diagnostic center and identified by subjecting the isolates to biochemical tests using standard procedures. Confirmed Enterobacteriaceae isolates were further subjected to screening for extended spectrum β-lactamases (ESBLs) production using Clinical Laboratory Standards Institute (CLSI) breakpoint and Double Disc Synergy Test (DDST). Standard discs of Augmentin (AUG 30 µg) {Optudisc} and Ceftriaxone (CXM 30 µg) {Medireich} and Ceftazidime (CAZ 30 µg) {Glaxo-Smithkline} were used in the screening. The results of CLSI breakpoint test showed that 87% were ESBLs producers viz: Citrobacter fruendii (3), Escherichia coli (46), Klebsiella pneumoniae (13), Morganella morganii (1), Proteus vulgaris (23) and Salmonella typhi (1) while that of confirmed ESBLs producers using DDST was 49%. These included Citrobacter fruendii (2), Escherichia coli (25), Klebsiella pneumoniae (7), Morganella morganii (1), Proteus vulgaris (13) and Salmonella typhi (1). The implication of the results is discussed.

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“…The results showed that 37 (9.25%) isolates were ESBL producers based on DDST while only 20 (5.00%) were positive using the NCCLS breakpoints (Yusha'u et al, 2007).…”

Section: Introductionmentioning

confidence: 98%

Prevalence of extended spectrum &#946-lactamases (ESBLs) among clinical <i>Enterobacteriaceae</i> isolates obtained from private diagnostic laboratory in Kano - Nigeria

Yusha’u¹,

Adakawa²,

Kawo³

2011

International Journal of Biological and Chemical Sciences

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“…However, they are most common in Klebsiella pneumoniae and Escherichia coli [6]. The prevalence varies worldwide even in closely related regions [7]. In Nigeria, an ESBL prevalence of 9.25% was recorded in a study conducted by Yusha'u et al, in Kano to screen for ESBLs production among isolates of Enterobacteriaceae [7].…”

Section: Introductionmentioning

confidence: 99%

“…The prevalence varies worldwide even in closely related regions [7]. In Nigeria, an ESBL prevalence of 9.25% was recorded in a study conducted by Yusha'u et al, in Kano to screen for ESBLs production among isolates of Enterobacteriaceae [7]. In another study conducted in a tertiary health centre in Ogun state, Nigeria to determine ESBL prevalence in Escherichia coli and Klebsiella Species; an ESBL prevalence of 2.5% for Escherichia coli and 5% for Klebsiella pneumoniae were recorded [8].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Extended- Spectrum Beta-lactamase from Escherichia coli and Klebsiella Species from North Eastern Nigeria

Mohammed¹

2016

JCDR

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Introduction: Resistance to antimicrobials has become a serious global health concern complicating treatment strategies and increasing health-care costs. The extended-spectrum betalactamase producing bacteria stand out as bacteria of great epidemic concern among Gram negative bacilli. Control and appropriate interventions for antimicrobial resistance depend on effective surveillance and knowledge of the patterns and determinants of resistance. Aim:The present study was undertaken to detect and characterize ESBLs in Escherichia coli and Klebsiella Species from University of Maiduguri Teaching Hospital, Maiduguri, North-Eastern Nigeria. Materials and Methods:Confirmed variants of Escherichia coli and Klebsiella Species isolated from 439 patients that were admitted in various units of University of Maiduguri Teaching Hospital (UMTH) were screened for ESBL using CLSI breakpoints. Suspected ESBLs producers were subjected to confirmation using double disk synergy method. Detection of ESBL genes was further done by multiplex PCR.Results: Out of the 439 isolates screened; the result shows 147 (33.5%) were ESBL producers but only 121(23.6%) were confirmed by the double disk synergy method. The prevalence of ESBL amongst the organisms were; 41/172 (23.8%) for Escherichia coli and 80/267/(30.0%) for Klebsiella Species. Based on PCR analysis, the various percentage genotypes of the ESBL producers were 44 (36.4%) for SHV gene followed by 38(31.4%) for TEM gene and the lowest of 33(27.3%) for CTX-M gene. Conclusion:ESBLs are prevalent among Species of Escherichia coli and Klebsiella Species in Maiduguri, Borno State, not only are there TEM and SHV but also CTX-M types.

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“…Another study conducted in a medical college hospital, Bangladesh found 41.66% ESBL in Gram-negative bacteria [11]. A number of studies in India have reported as ESBL producers were 40.85%, 51.4% and 53.84% in 2004, 2007 and in 2010, respectively [12][13][14]. However, another two studies in Iran and India documented high prevalence 96% and 97%, respectively [15,16].…”

Section: Introductionmentioning

confidence: 99%

Detection of Extended Spectrum β-lactamases Producing Genes among Third Generation Cephalosporins Sensitive Bacterial Strains from a Medical College Hospital in Bangladesh

T¹

2014

ADT

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Background: Extended Spectrum β-lactamases (ESBLs) are rapidly evolving group of β-lactamase enzymes produced by the gram-negative bacteria which is very important to detect in clinical laboratory for effective treatment. The aim of the present study was to see the ESBL genes among Third Generation Cephalosporins (3GCs) sensitive bacterial strains. Methods:This cross sectional study was undertaken in non-repetitive clinical isolates collected from Mymnesingh Medical College Hospital, Mymensingh, Bangladesh from both the outpatient and inpatient departments over a period of six months from January 2011 to June 2011. The ESBL status was confirmed by double disc diffusion test and minimum inhibitory concentration by agar dilution method as recommended by Clinical and Laboratory Standard Institute 2010 and multiplex polymerase chain reaction for TEM, SHV and CTX-M genes.Results: A total of 300 Gram negative bacilli were included in the study; among them 236 were resistant and 64 were sensitive to 3GCs by disc diffusion test. Multidrug resistant ESBL production was found 75.8% from resistant isolates and 54.6% from sensitive isolates. Rate of TEM, SHV and CTX-M genes present among sensitive strains were 36.4%, 24.2% and 21.1%, respectively. Conclusion:Both common and new ESBLs genes were detected from 3GCs sensitive bacteria of which TEM is most common. Routine ESBLs screening for all Gram-negative isolates both from sensitive and resistant to 3GCs strains might be useful for the physicians in selecting effective antibiotics.

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Prevalence of extended spectrum β-lactamases (esbls) among enterobacteriaceae in murtala mohammed specialist hospital, Kano, Nigeria

Cited by 39 publications

References 10 publications

Prevalence of extended spectrum &#946-lactamases (ESBLs) among clinical <i>Enterobacteriaceae</i> isolates obtained from private diagnostic laboratory in Kano - Nigeria

Prevalence of extended spectrum &#946-lactamases (ESBLs) among clinical <i>Enterobacteriaceae</i> isolates obtained from private diagnostic laboratory in Kano - Nigeria

Characterization of Extended- Spectrum Beta-lactamase from Escherichia coli and Klebsiella Species from North Eastern Nigeria

Detection of Extended Spectrum β-lactamases Producing Genes among Third Generation Cephalosporins Sensitive Bacterial Strains from a Medical College Hospital in Bangladesh

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