Prevalence of Genital Human Papilloma Virus Infection and Genotypes among Young Women in Sicily, South Italy

Ammatuna, Pietro; Giovannelli, Lucia; Matranga, Domenica; Ciriminna, Saverio; Perino, Antonio

doi:10.1158/1055-9965.epi-08-0180

Cited by 41 publications

(43 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These findings are consistent with the HPV DNA prevalence rates reported by other Italian groups (1,6,14,51) and match the rates of prevalence from combined DNA-and RNA-based studies (2,7,10,13,24). As reported earlier, the study detected a number of mixed infections ( Table 4).…”

Section: Discussionsupporting

confidence: 92%

Clinical Performance of Human Papillomavirus E6 and E7 mRNA Testing for High-Grade Lesions of the Cervix

et al. 2009

View full text Add to dashboard Cite

Infection with high-risk (HR) human papillomavirus (HPV) is the major cause of cervical cancer. However, relatively few infections progress to malignant disease. Progression to malignancy requires the overexpression of the E6 and E7 genes in the integrated HPV genome. It follows that the E6 and E7 transcripts could be useful markers of disease progression. The study presented here tests this possibility, using data from colposcopy and from cytological and histological tests to compare RNA assays for the E6 and E7 genes with DNA testing. A total of 180 women underwent colposcopy, cytology, and biopsy of suspected lesions (143 cases). Cervical brush specimens were analyzed for HPV DNA and for E6 and E7 mRNA. DNA from HR HPV was found in 57.8% of the specimens; E6 and E7 transcripts were found in 45%. The rates of detection of HPV DNA and of E6 and E7 transcripts were 33.3% and 25%, respectively, for specimens with normal findings; 51.4% and 31.9%, respectively, for specimens with cervical intraepithelial neoplasia grade 1 (CIN1); and 61.1% and 44.2% for specimens with CIN2, respectively. All specimens with CIN3 and 95.5% of specimens from patients with squamous cell carcinoma were positive by both assays. Thirty-seven patients with normal colposcopy findings did not undergo biopsy. HPV DNA and mRNA transcripts were found in 32.4% and 18.9% of these cases, respectively. Comparisons with cytological tests produced similar results. Overall, the mRNA tests showed a higher specificity than the DNA tests for high-grade lesions (72.7% and 56.2%, respectively) and a higher positive predictive value (59.3% and 49.0%, respectively). These findings suggest that mRNA assays could be more powerful than DNA testing for predicting the risk of progression and offer a strong potential as a tool for triage and patient follow-up.Carcinomas of the anogenital tract, particularly cancer of the cervix, represent the second most frequent type of neoplasm worldwide (43, 57). The major cause of these cancers is infection with high-risk (HR) human papillomavirus (HPV). DNA from HPV has been detected in more than 99% of cervical squamous cell carcinomas (SCCs) and a smaller proportion of adenocarcinomas (3,4,31,33). However, most HPV infections regress spontaneously or progress only after a long period of latency. As a result, the number of infections is far higher than the number of women who develop cancer.The most common types of HPV found in cancer patients are types 16, 18, 31, 33, and 45 (5, 10, 40, 53). Persistent infection with these types is regarded as a significant risk factor (40). The role of HPVs in the etiology of cervical cancer is tightly correlated with the overexpression of two oncogenes (E6 and E7) due to a specific opening in the E2 open reading frame in the integrated viral genome (23, 28). Studies of cervical cancer cell lines and cancer biopsy specimens have shown that the continuous expression of the genes is a necessary condition for the transformation and maintenance of neoplastic and dysplastic cells (46,56,57).Ce...

show abstract

Section: Discussionsupporting

confidence: 92%

Clinical Performance of Human Papillomavirus E6 and E7 mRNA Testing for High-Grade Lesions of the Cervix

et al. 2009

View full text Add to dashboard Cite

show abstract

“…This finding is similar with data reported in other Italian areas, Germany and Denmark, 21,26,27,34 reflecting a higher risk in young sexually active women who, however, mostly have a transient and asymptomatic infection, with a spontaneous HPV clearance in 70-90% of cases within 12-13 months 24 …”

Section: Discussionsupporting

confidence: 91%

“…The second most frequent type was HPV-53 (15.7%) in accordance with other Italian 34 and Spanish studies, 28,39,40 both in general population and in females with abnormal smears. In fact, although it is not recurrent among the most prevalent types worldwide, it was found as the second and the fifth, 21,30 most frequent genotype isolated in Italian studies which considered general female population and females with abnormal smears.…”

Section: Discussionsupporting

confidence: 90%

See 1 more Smart Citation

Prevalence and Molecular Epidemiology of Human Papillomavirus Infection in Italian Women with Cervical Cytological Abnormalities

Meloni

Pilia

Campagna

et al. 2014

Journal of Public Health Research

View full text Add to dashboard Cite

BackgroundHuman papillomavirus (HPV) infection is the most common sexually transmitted infection and high-risk HPV types are a necessary cause for the development of cervical cancer. The present study investigated the HPV-type specific prevalence in 650 women, aged 15-76 years, with cytological abnormalities and the association between HPV infection and cervical disease in a subset of 160 women for whom cytological results for Pap-Test were available, during the period 2008-2011 in Cagliari (Southern Italy).Design and MethodsHPV-DNA extraction was performed by lysis and digestion with proteinase K and it was typed by using the INNOLiPA HPV Genotyping Assay.ResultsOverall the HPV prevalence was 52.6%; high-risk genotypes were found in 68.9% of women and multiple-type infection in 36.1% of HPV-positive women. The commonest types were HPV-52 (23.4%), HPV-53 (15.7%), HPV-16 (15.4%) and HPV-6 (12.4%). Among the women with cytological diagnosis, any-type of HPV DNA was found in 49.4% of the samples and out of these 93.7% were high-risk genotypes. Genotype HPV 53 was the commonest type among women affected by ASCUS lesions (21.4%), genotype 52 in positive L-SIL cases (22.5%), genotype 16 H-SIL (27.3%).ConclusionsThis study confirmed the high prevalence of HPV infection and high-risk genotypes among women with cervical abnormalities while, unlike previously published data, genotype HPV-52 was the most common type in our series. These data may contribute to increase the knowledge of HPV epidemiology and designing adequate vaccination strategies.Significance for public healthHuman papillomavirus (HPV) is the most common sexually-transmitted agent, which can cause cervical lesions and cancer in females. Efforts to reduce the burden of cervical cancer with cytology screening in the last years have had limited success. HPV infection and disease imposes a substantial burden of direct costs on the Italian National Health Service that have never been fully quantified. Monitoring HPV prevalence could represent a tool to follow the evolution of the infection in the vaccination and post-vaccination era, to understand the impact of HPV types in cervical diseases in Italy. Our survey shows an high frequency of infections sustained by HPV 52. Given the recent implementation of a widespread immunization program with vaccines not containing HPV 52, it has been relevant to prove the high prevalence of this HPV genotype from the beginning of the vaccination campaign, to avoid ascribing to the vaccination program a possible selection effect and the importance of non-vaccine HPV types in the burden of cervical disease, in order to assess the opportunity to realize new vaccine including other types.

show abstract

“…These risk factors are either biological, such as co-infections with other sexually transmitted diseases (STDs), or behavioral, such as sexual habits. A consistent association has been demonstrated between an increased lifetime number of sexual partners and HPV infection among women [4][5][6]. However, a number of other factors, such as young age at first sexual intercourse, use of oral contraceptives, and smoking have shown inconsistent associations with HPV infection [6][7][8][9].…”

Section: Introductionmentioning

confidence: 90%

Risk factors for human papillomavirus infection among women in Portugal: The CLEOPATRE Portugal Study

Pista

Oliveira

Cunha³

et al. 2012

Intl J Gynecology & Obste

View full text Add to dashboard Cite

show abstract

Prevalence of Genital Human Papilloma Virus Infection and Genotypes among Young Women in Sicily, South Italy

Cited by 41 publications

References 22 publications

Clinical Performance of Human Papillomavirus E6 and E7 mRNA Testing for High-Grade Lesions of the Cervix

Clinical Performance of Human Papillomavirus E6 and E7 mRNA Testing for High-Grade Lesions of the Cervix

Prevalence and Molecular Epidemiology of Human Papillomavirus Infection in Italian Women with Cervical Cytological Abnormalities

Risk factors for human papillomavirus infection among women in Portugal: The CLEOPATRE Portugal Study

Contact Info

Product

Resources

About