Prevalence of Helicobacter pylori vacA, cagA, iceA and oipA genotypes in Tunisian patients

Mansour, K. Ben; Fendri, C.; Zribi, M.; Masmoudi, A.; Labbène, M.N.; Fillali, Azza; Mami, N. Ben; Najjar, Taoufik; Meherzi, A.; Sfar, T.; Burucoa, Christophe

doi:10.1186/1476-0711-9-10

Cited by 71 publications

(63 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…H. pylori strains from human biopsy specimens were cultured, and 24-h cultures were used for DNA extraction using a MagNA Pure compact system and DNA isolation kit (Roche) according to the manufacturer's instructions. A PCR was then performed to determine cagA and cagM status as previously described (18,19). In clinical isolates, the presence of cagA and cagM was used as a marker of the presence of the cag PAI.…”

Section: Methodsmentioning

confidence: 99%

Chemokines and Antimicrobial Peptides Have a cag -Dependent Early Response to Helicobacter pylori Infection in Primary Human Gastric Epithelial Cells

et al. 2014

Self Cite

View full text Add to dashboard Cite

e Helicobacter pylori infection systematically causes chronic gastric inflammation that can persist asymptomatically or evolve toward more severe gastroduodenal pathologies, such as ulcer, mucosa-associated lymphoid tissue (MALT) lymphoma, and gastric cancer. The cag pathogenicity island (cag PAI) of H. pylori allows translocation of the virulence protein CagA and fragments of peptidoglycan into host cells, thereby inducing production of chemokines, cytokines, and antimicrobial peptides. In order to characterize the inflammatory response to H. pylori, a new experimental protocol for isolating and culturing primary human gastric epithelial cells was established using pieces of stomach from patients who had undergone sleeve gastrectomy. Isolated cells expressed markers indicating that they were mucin-secreting epithelial cells. Challenge of primary epithelial cells with H. pylori B128 underscored early dose-dependent induction of expression of mRNAs of the inflammatory mediators CXCL1 to -3, CXCL5, CXCL8, CCL20, BD2, and tumor necrosis factor alpha (TNF-␣). In AGS cells, significant expression of only CXCL5 and CXCL8 was observed following infection, suggesting that these cells were less reactive than primary epithelial cells. Infection of both cellular models with H. pylori B128⌬cagM, a cag PAI mutant, resulted in weak inflammatory-mediator mRNA induction. At 24 h after infection of primary epithelial cells with H. pylori, inflammatory-mediator production was largely due to cag PAI substrate-independent virulence factors. Thus, H. pylori cag PAI substrate appears to be involved in eliciting an epithelial response during the early phases of infection. Afterwards, other virulence factors of the bacterium take over in development of the inflammatory response. Using a relevant cellular model, this study provides new information on the modulation of inflammation during H. pylori infection.

show abstract

Section: Methodsmentioning

confidence: 99%

Chemokines and Antimicrobial Peptides Have a cag -Dependent Early Response to Helicobacter pylori Infection in Primary Human Gastric Epithelial Cells

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…In a study conducted by Mansour and colleagues (17), there was no significant association between cagA gene and sex, but the association was statistically significant among the vacA, iceA and oipA genes (17). …”

Section: H Pylori Genotypesmentioning

confidence: 99%

Prevalence of genotypes in Helicobacter pylori isolates from patients in eastern Turkey and the association of these genotypes with clinical outcome

Ozbey¹,

Aygün²

2012

Braz. J. Microbiol.

View full text Add to dashboard Cite

There is not much information available regarding the prevalence of the genotypes of Helicobacter pylori isolates in Turkey, particularly in eastern Turkey. The aims of this study were to detect the prevalence of different genotypes of H. pylori in Turkish patients with gastrointestinal complaints and to determine the relationship of these genotypes with clinical outcome and sex. One hundred forty H. pylori isolates were examined for the presence of its genotypes by the PCR. We found that the prevalence of vacAs1, vacAs2, cagA, cagE, iceA1, iceA2 and babA2 genes were 88.6%, 11.4%, 71.4%, 35.7%, 41.4%, 58.6%, and 62.1%, respectively. The most predominant vacA subtype was s1a (81.4%). The most vacA allelic combination detected were vacAs1m1 (65.2%) and s1m2 (53.9%) in patients with peptic ulcer and gastritis, respectively.The only vacAs1 isolate was significantly associated with gastritis and peptic ulcer (p<0.05). The vacAs1a, ml, slml and babA2 genes were significantly associated with peptic ulcer (p<0.05), whereas m2 gene was significantly associated with only gastritis (p<0.05). The difference between sex and genotypes was statistically significant among the cagA, vacAs1, iceA2 and babA2 genes. This study reported for the first time the prevalence of H. pylori genotypes in patients with gastrointestinal complaints in eastern Turkey.Further studies are needed to understand epidemiological importance of the genotypes of H. pylori isolates in this region and the association between the virulence genes and clinical outcome in different regions.

show abstract

“…Multiplex PCR was used to study the distribution of vacA (s1a, s1b, s1c, s2, m1a, m1b and m2) and cagA genotypes. List of primers and PCR condition is shown in Table 1 (14,15). All runs were done using a programmable thermal cycler (Eppendorf-Netheler-Hinz GmbH, Germany) PCR device.…”

Section: Genotyping Of Vaca and Caga Genotypesmentioning

confidence: 99%

Prevalence and Genotyping of Helicobacter pylori Isolated From Meat, Milk and Vegetable in Iran

Talimkhani

Mashak

2017

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Despite the considerable clinical role of Helicobacter pylori, its certain routes of transmission and origin have not been reported. Based on the argumentative hypothesis, foods play an imperative role in the spread of H. pylori to humans. Objectives: The current research was done to investigate the prevalence rate and distribution of Vacuolating Cytotoxin A and Cytotoxin Associated Gene A genotypes in the H. pylori strains isolated from meat, milk, and vegetables. Methods: A total of 340 food samples were collected and directly moved to the laboratory. Samples were cultured and H. pylori colonies were approved using the gram staining, urease test, and 16s rRNA-based polymerase chain reaction (PCR) amplification. Positive strains were tested for distribution of vacA and cagA genotypes using the multiplex-PCR. Results: Out of 340 samples, 40 (11.76%) harbored H. pylori. Prevalence of H. pylori in meat, milk, and vegetable samples were 7.33%, 16%, and 12.50%, respectively. Ovine milk (26%) was the most commonly contaminated sample. The most commonly detected genotypes were vacA s1a (87.50%), vacA m1a (87.50%), vacA s2 (82.50%), cagA (80%), and vacA m2 (62.50%). Genotypes of S1am1a (62.50%), s2m1a (55%), s1am2 (50%), s2m2 (45%), and m1am2 (42.50%) were the most commonly detected combined genotypes. Conclusions: Milk, vegetables, and meat, are latent sources of H. pylori. Similarity in the genotyping pattern of H. pylori strains of various samples represents their similar sources of infection. Further studies are required for finding the exact sources of H. pylori strains.

show abstract

Prevalence of Helicobacter pylori vacA, cagA, iceA and oipA genotypes in Tunisian patients

Cited by 71 publications

References 35 publications

Chemokines and Antimicrobial Peptides Have a cag -Dependent Early Response to Helicobacter pylori Infection in Primary Human Gastric Epithelial Cells

Chemokines and Antimicrobial Peptides Have a cag -Dependent Early Response to Helicobacter pylori Infection in Primary Human Gastric Epithelial Cells

Prevalence of genotypes in Helicobacter pylori isolates from patients in eastern Turkey and the association of these genotypes with clinical outcome

Prevalence and Genotyping of Helicobacter pylori Isolated From Meat, Milk and Vegetable in Iran

Contact Info

Product

Resources

About