Prevalence of HPV Infection in Head and Neck Cancer Patients in Egypt: National Cancer Institute Experience.

Abstract

Background: The new classification of Head and Neck Squamous Cell Carcinoma (HNSCC) according to their association with human papillomavirus (HPV) infection into HPV positive tumors with good prognosis and HPV negative tumors with poor prognosis has changed the treatment strategy of HNSCCs dramatically worldwide. However, data concerning HPV infection in HNSCCs patients is still limited and there are no studies regarding HPV genotypes in HNSCC among Egyptians. Therefore, the aim of the current study is to investigate the frequency of HPV and to detect the most prevalent HPV genotypes in the patients with oral cavity and oropharyngeal carcinoma from EgyptMethods: This cross sectional study was conducted from January 2016 to November 2017. Ninety-two oral brushing samples (76 oral cavity and 16 oropharynx) were collected by cytobrushing in CYTO-fast solution. HPV-DNA was subjected to two methods which are the: (1) HPV Direct Flow CHIP system for automatic detection and genotyping (2) conventional PCR for detection of HPV using consensus primers (MYO9/MYO11). SPSS software was used for statistical analysis.Results: Out of the 92 cases assessed, HPV DNA was present in three samples only (3/92; 3.3%) and the HPV66 genotype was the only genotype detected by HPV Direct Flow CHIP System.Conclusion: The Results of this study may (1) indicate that the HPV-associated HNSCC among Egyptian patients is very low and (2) highlight the presence of HPV66 genotype in these malignancies. Other studies from other countries in general and from different governorates in Egypt are still needed to determine the exact frequency of HPV infections in head and neck cancer.

Role of P16, P53 and Cyclin D1 in Detection of HPV Induced Laryngeal Squamous Cell Carcinoma: An Immunohistochemical Study

“…1 Head and Neck Squamous Cell Carcinomas, include cancers of the aero-digestive tract, an estimated annual burden of thousands of deaths, and more than 650,000 incident cases, making it the sixth most common cancer worldwide, studies have shown that Head & Neck Squamous cell carcinomas (HNSCCs) responsible for about 20% of all cancers. 2,3 In Egypt, the Cancer Pathology Registry of the National Cancer Institute of Cairo University declared that during the years 2003/2004 laryngeal cancer was the most frequent among all respiratory cancers (27.84%) and the tenth most frequent among all cancers (1.77%), with most cases diagnosed at advanced stages. 4,5 Laryngeal cancer can result from the combined action of smoking, drinking alcohol, air pollution, and gastroesophageal reflux.…”