Prevalence of Human Papillomavirus DNA in Different Histological Subtypes of Cervical Adenocarcinoma

Abstract

The prevalence of human papilloma virus (HPV) DNA in different histological subtypes of cervical adenocarcinoma and related tumors was examined using formalin-fixed, paraffin-embedded tissue samples from 105 primary cervical adenocarcinomas and adenosquamous carcinomas. Broad-spectrum HPV DNA amplification and genotyping was performed with the SPF10 primer set and line probe assay (LiPA), respectively. HPV DNA was detected in 82 of 90 (91%) mucinous adenocarcinomas, encompassing endocervical, intestinal, and endometrioid histological subtypes, and in nine of nine adenosquamous tumors (100%). HPV DNA was not detected in any nonmucinous adenocarcinomas including clear cell, serous, and mesonephric carcinomas (0/6). The most common viral types detected in adenocarcinoma were HPV 16 (50%) and HPV 18 (40%), followed by HPV 45 (10%), HPV52 (2%), and HPV 35 (1%). Multiple HPV types were detected in 9.7% of the cases. Adenocarcinoma of the cervix (AdCx) accounts for approximately 15% of cervical cancers and has been increasing in incidence during the last few decades, particularly in younger women. 1 The etiology of squamous cell carcinoma of the cervix, the most common type of cervical malignancy, is linked to infection with oncogenic types of human papillomavirus (HPV), but the pathogenesis of AdCx is less well understood. Although HPV DNA is consistently detected in Ͼ90% of squamous cell carcinomas of the cervix, 2 the reported prevalence of HPV DNA in AdCx varies significantly, from 32% to 100%, depending on the detection method used. [3][4][5][6][7][8][9][10][11][12][13]

Prevalence of human papillomavirus DNA in various histological subtypes of cervical adenocarcinoma: a population-based study

“…5,6 However, the recent more sensitive techniques have made it possible to identify the higher rate of HPV infection in adenocarcinoma with a frequency of 85% or more. [19][20][21] In this study, we used a recently developed HPV DNA Chip for the detection of HPV DNA in 82 of 135 cases. This method is based on the polymerase chain reaction with an advantage to detect single and multiple infection of 22 HPV genotypes at once and has reported to be a very sensitive method comparable with Hybrid Capture II assay.…”

“…This result was in accordance with the recent reports by other investigators of Western countries that showed the similar prevalence rate between HPV 16 and HPV 18 in cervical adenocarcinoma. 6,20,23 Accordingly, it is very clear that HPV 18 has a stronger association with cervical adenocarcinoma than with cervical squamous cell carcinoma, where the frequency of HPV 18 (11.4-13.7%) was much less common than that of the cervical adenocarcinoma. Bearing in mind that HPV 18-related cervical carcinomas were associated with poor prognostic factors or poor survival, 24,25 the high frequency of HPV 18 in cervical adenocarcinoma may be implicated in the poorer 5-year survivals of cervical adenocarcinoma compared to squamous cell carcinoma with the same stages as reported by several investigators.…”

Incidence and survival rate of women with cervical cancer in the Greater Amsterdam area

“…Since more than 92% of the adenocarcinomas and its precursors contain hrHPV (Pirog et al, 2000;Pilch et al, 2001), adding hrHPV testing to conventional cytological screening might improve the present screening programme in detecting adenocarcinoma and its precursor lesions. This should be a focus of further research concerning the functioning of the screening programme for cervical cancer.…”

Human Papillomavirus-16 Associated Adenocarcinoma NOS of Base of Tongue

“…This situation resembles the role played by HPV in the carcinogenesis of uterine cervix where HPV is found not only in squamous cell carcinoma [30], but also in adenocarcinomas [31], and neuroendocrine carcinomas [32,33].…”