Prevalence of human papillomavirus in archival samples obtained from patients with cervical pre-malignant and malignant lesions from Northeast Brazil

Abstract

BackgroundHuman Papillomavirus (HPV) is considered as a necessary, but not sufficient, cause of cervical cancer. In this study, we aimed to assess the prevalence of HPV in a series of pre-malignant and malignant cervical lesion cases, to identify the virus genotypes, and to assess their distribution pattern according to lesion type, age range, and other considered variables. The samples were submitted to histopathological revision examination and analysed by polymerase chain reaction (PCR) for the presence of HPV DNA, followed by HPV typing by dot blot hybridisation.FindingsOf the analysed samples, 53.7% showed pre-malignant cervical lesions, and 46.3% presented with cervical cancer. Most cancer samples (84.1%) were classified as invasive carcinoma. The mean age of these cancer patients was 47.3 years. The overall HPV prevalence was 82.4% in patients with pre-malignant lesions and 92.0% in the cancer patients. HPV 16 was the most prevalent type, followed by HPV 18 and 58, including both single and double infections. Double infection was detected in 11.6% of the samples, and the most common combination was HPV 16+18.ConclusionsCervical cancer appears to occur in women in a lower age range in the studied area, compared to the situation in other Brazilian regions. Furthermore, among the patients with CIN 3 and those with cancer, we observed a higher proportion of married women, women with more than one sexual partner, smokers, and individuals with less than an elementary education, relative to their counterparts.FindingsThe overall HPV prevalence was 82.4% in patients with pre-malignant lesions and 92.0% in the cervical cancer patients from Northeast Brazil. HPV 16 was the most prevalent type, followed by HPV 18 and 58. The most common double infection was HPV 16+18. Cervical cancer appears to occur in women in a lower age range in the Northeast Brazil. Among the patients with CIN 3 and those with cancer, we observed a higher proportion of married women, women with more than one sexual partner, smokers, and individuals with less than an elementary education, relative to their counterparts.

Prevalence of human papillomavirus (HPV), distribution of HPV types, and risk factors for infection in HPV-positive women

“…Several investigations have been carried out on the prevalence and distribution of HPV in different regions of Brazil (Villa et al, 2000;Sichero et al, 2007;de Medeiros Fernandes et al, 2008;Baldez da Silva et al, 2009;Fernandes et al, 2009Fernandes et al, , 2010Castro et al, 2011;Oliveira-Silva et al, 2011;Freitas et al, 2014;Chagas et al, 2015;Gurgel et al, 2013Gurgel et al, , 2015. The results of these studies have shown divergent HR HPV genotype distribution, although most have pointed to HPV16 as the most frequent type (with the exception of the study developed by Chagas et al, 2015).…”

Cervical Precancerous Lesions and Cancer among Patients in the Gynaecology Outpatient Department at a Tertiary Hospital in South Africa

“…Moyer (2012) reported that CC most commonly occurs in women age 35-55 years. A Brazilian study conducted to determine the Prevalence of human papillomavirus from patients with cervical pre-malignant and malignant lesions found the mean age of CC among these women was 47.3 years (Fernandes et al, 2010).…”

Infecção por Papilomavírus Humano de alto risco Oncogênico em mulheres atendidas no Programa de Saúde da Família da Cidade de Serra Talhada, Pernambuco

“…Noronha et al (2011) avaliaram HR-HPV em 1021 amostras de mulheres com citologia negativa, sendo detectado um percentual de 12,7% do HPV 18, seguido do HPV-16 em 7% e do HPV 31 em 5,6%. Fernandes et al (2010) avaliaram o HPV 16 como mais prevalente em 215 mulheres com citologia normal e com lesões intraepiteliais cervical de baixo grau (LSIL) atendidas num serviço de screening de câncer cervical em Natal-RN, Brasil. Outros estudos realizados no Brasil encontraram prevalência semelhante: no sul, 24,6% em 1.500 mulheres; no nordeste de 24,5% em 1020 mulheres; e no norte 25,3% em 265 mulheres (Rosa et al, 2008;Noronha et al, 2011;Vieira et al, 2015).…”