Prevalence of<i>Trypanosoma cruzi</i>in Dogs (<i>Canis familiaris</i>) and Triatomines During 2008 in a Sanitary Region of the State of Mexico, Mexico

Barbabosa‐Pliego, Alberto; Gil, Pablo Campos; Hernández, D. O.; Aparicio-Burgos, José Esteban; Jiménez, Roberto Montes de Oca; Martínez-Castañeda, José Simón; Ochoa-García, Laucel; Guzmán-Bracho, Carmen; Estrada-Franco, José G.; Garg, Nisha; Vázquez-Chagoyán, Juan Carlos

doi:10.1089/vbz.2009.0163

Cited by 24 publications

(32 citation statements)

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“…Dogs were confirmed free of T. cruzi infection by microscopic examination of blood smears and serological evaluation of anti- T. cruzi antibodies using an indirect haemagglutination assay (IHA) and enzyme-linked immunosorbent assay (ELISA) [15], [18]. Before inclusion in experimental studies, dogs were treated with anti-helminthes and vaccines against regional infectious diseases (Canine distemper, Parvovirus infection, Canine hepatitis, Leptospirosis, and Rabies).…”

Section: Methodsmentioning

confidence: 99%

“…We chose dogs for our studies because dogs provide an excellent model for studying the human disease [11]–[13]. Experimentally and naturally infected young dogs (2–3 months) elicit reproducible and comparable acute infection associated with increase in blood parasitemia, IgG and IgM antibodies [14], [15] and T cell response [16]. The presence of myocarditis with a moderate or small number of parasitized cells and extensive and frequent focal necrosis turns the disease in dogs similar to acute Chagas disease in humans [17], [18].…”

Section: Introductionmentioning

confidence: 99%

“…rural Argentina, Chiapas, Mexico), determined by serological procedures and xenodiagnosis [24], [25]. Dogs are also the most frequent blood meal source for the domestic triatomines, i.e., T. barberi and T. pallidipennis in Mexico [15] and T. infestans in Argentina [25], [26]. Likewise, a high prevalence of seropositive dogs and infected triatomines is routinely noted in rural and urban development in the southern US [27], [28], and suggested to maintain T. cruzi transmission in the human habitat.…”

Section: Introductionmentioning

confidence: 99%

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Testing the Efficacy of a Multi-Component DNA-Prime/DNA-Boost Vaccine against Trypanosoma cruzi Infection in Dogs

et al. 2011

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Background Trypanosoma cruzi, the etiologic agent of Chagas Disease, is a major vector borne health problem in Latin America and an emerging infectious disease in the United States.MethodsWe tested the efficacy of a multi-component DNA-prime/DNA-boost vaccine (TcVac1) against experimental T. cruzi infection in a canine model. Dogs were immunized with antigen-encoding plasmids and cytokine adjuvants, and two weeks after the last immunization, challenged with T. cruzi trypomastigotes. We measured antibody responses by ELISA and haemagglutination assay, parasitemia and infectivity to triatomines by xenodiagnosis, and performed electrocardiography and histology to assess myocardial damage and tissue pathology.ResultsVaccination with TcVac1 elicited parasite-and antigen-specific IgM and IgG (IgG2>IgG1) responses. Upon challenge infection, TcVac1-vaccinated dogs, as compared to non-vaccinated controls dogs, responded to T. cruzi with a rapid expansion of antibody response, moderately enhanced CD8+ T cell proliferation and IFN-γ production, and suppression of phagocytes’ activity evidenced by decreased myeloperoxidase and nitrite levels. Subsequently, vaccinated dogs controlled the acute parasitemia by day 37 pi (44 dpi in non-vaccinated dogs), and exhibited a moderate decline in infectivity to triatomines. TcVac1-immunized dogs did not control the myocardial parasite burden and electrocardiographic and histopatholgic cardiac alterations that are the hallmarks of acute Chagas disease. During the chronic stage, TcVac1-vaccinated dogs exhibited a moderate decline in cardiac alterations determined by EKG and anatomo-/histo-pathological analysis while chronically-infected/non-vaccinated dogs continued to exhibit severe EKG alterations.ConclusionsOverall, these results demonstrated that TcVac1 provided a partial resistance to T. cruzi infection and Chagas disease, and provide an impetus to improve the vaccination strategy against Chagas disease.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Testing the Efficacy of a Multi-Component DNA-Prime/DNA-Boost Vaccine against Trypanosoma cruzi Infection in Dogs

et al. 2011

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“…La infección por T. cruzi en perros se ha demostrado en diversos países de Latinoamérica y en los Estados Unidos (13)(14)(15)(16). Los perros se consideran un indicador de la transmisión activa, ya que los cánidos infectados y la presencia de vectores incrementan el riesgo para las personas; de hecho, la presencia de un perro infectado en una vivienda cuadruplica el riesgo de la infección en niños (17,18).…”

Section: Discussionunclassified

Seroprevalencia de la infección por Trypanosoma cruzi en Canis familiaris del estado Sucre, Venezuela

et al. 2012

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Contribución de los autores:Mariolga Berrizbeitia: coordinadora del proyecto a nivel regional, realización del análisis estadístico y escritura del manuscrito. Juan Luis Concepción: coordinador nacional del proyecto, revisión y aprobación del manuscrito. Valentina Cazorla: realización de los ensayos, contribución en la escritura del manuscrito. Jessicca Rodríguez: realización de los ensayos. Ana Cáceres y Wilfredo Quiñones: asesoría en la elaboración del proyecto, revisión del análisis estadístico. . No significant statistic association was found between the T. cruzi infection in dogs and the evaluated epidemiological variables: hunting dogs that slept oudoors roaming freely in the populated center, sex of the animal and eating habits. The T. cruzi infection was associated to the age of canines, being significantly higher in the group of 0 to 3 years, when compared with older dogs. ARTÍCULO ORIGINAL

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“…Additionally reported are the following triatomine vector species: Meccus pallidipennis, T. dimidiata, and T. barberi. Furthermore, Barbabosa-Pliego et al 9 also determined the prevalence of T. cruzi infection in 24% of dogs and 35% of triatomines collected in the sanitary region located in the Southern part of the Estado de Mexico. In the Toluca Valley, Estado de Mexico, Quijano-Hernández et al 10 performed an epidemiological study in non-domiciliary and domiciliary dogs and found no evidence of T. cruzi in local domiciliary dogs or triatomines.…”

mentioning

confidence: 99%

Seroprevalence of human Trypanosoma cruzi infection in the North of Estado de Mexico

González-Guzmán

Pichardo-Ávila²,

Mimbrera-Rodríguez³

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction: Chagas disease is a neglected public health problem in Mexico; however, detailed studies to determine the seroprevalence in some states have not been performed. Methods: A total 1,504 human serum from thirteen communities in Estado de Mexico, were analyzed with three diagnostics techniques. Results: The overall seroprevalence was 9.1%, with high prevalence among people aged 51-60 years, while people aged 0-29 years were seronegative against T. cruzi. Conclusions: Our data demonstrated the seroprevalence of T. cruzi in the North of the Estado de Mexico, an area considered as non-endemic; however, epidemiological conditions necessary for natural transmission were found.

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Prevalence ofTrypanosoma cruziin Dogs (Canis familiaris) and Triatomines During 2008 in a Sanitary Region of the State of Mexico, Mexico

Cited by 24 publications

References 21 publications

Testing the Efficacy of a Multi-Component DNA-Prime/DNA-Boost Vaccine against Trypanosoma cruzi Infection in Dogs

Testing the Efficacy of a Multi-Component DNA-Prime/DNA-Boost Vaccine against Trypanosoma cruzi Infection in Dogs

Seroprevalencia de la infección por Trypanosoma cruzi en Canis familiaris del estado Sucre, Venezuela

Seroprevalence of human Trypanosoma cruzi infection in the North of Estado de Mexico

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