Prevalence of Listeria monocytogenes in delicatessen food products in China

Guoxiang, Chao; Deng, Yunfei; Zhou, Xiaohui; Xu, Qin; Qian, Xiaoqin; Zhang, Liping; Zhu, Binyang

doi:10.1016/j.foodcont.2005.06.016

Cited by 13 publications

(10 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…L. monocytogenes phospholipases are essential determinants of pathogenicity and thus, we investigated them as well (5). plcA and hlyA existed in all the positive cases (16). In a study by Kaur et al the prevalence of L. monocytogenes in India reported four isolates from 305 samples comprising blood, urine, and placental bits, faecal and vaginal swabs that were collected from 61 patients with spontaneous abortion samples.…”

Section: Discussionmentioning

confidence: 97%

Identification of Listeria monocytogenesVirulence Factors in Women With Abortion by Polymerase Chain Reaction

Eslami¹,

Goudarzi²,

Ohadi³

et al. 2014

Arch Clin Infect Dis

View full text Add to dashboard Cite

Background: Listeria monocytogenes, as one of the foodborne pathogens, is a causative agent of listeriosis. The transfer of L. monocytogenes bacteremia in pregnant women occurs as self-limited flu-like symptoms, but it may result in abortion, stillbirth or premature birth of the infected baby. One of the best methods for detection of this bacterium is polymerase chain reaction (PCR). Objectives: The purpose of this study was detection of virulence factors (hlyA and plcA) of L. monocytogenes in women with abortion, using PCR. Patients and Methods: In this pilot and cross-sectional study, 96 patients with abortion admitted in educational university, Tehran, Iran were surveyed for L. monocytogenes by PCR and culture methods. Some variants like age, occupation, history of abortion and education were considered for all patients. Vaginal swabs and secretions were transferred to trypticase soy broth as the transport media and then all the samples were transferred to a microbiology laboratory. The tubes were incubated in 4 ºC and the specimens were cultured on PALCAM media. The isolates were verified by Gram staining, catalase and oxidase test, methyl red-Voges-Proskauer (MR-VP), sugar fermentations and motility in 20-25ºC. Then, PCR was performed for the extracted DNAs. Data were analyzed by SPSS software version 17, and χ2 (Chisquare test). Results: Out of 96 samples, 16 isolates of L. monocytogenes by PCR (plcA and hlyA) and four isolates by culture were identified. There was a significant difference between PCR and culture methods (P = 0.003). The results of this study showed that PCR was more sensitive and specific than culture method. There was also a significant association between the bacteria and hlyA and plcA genes and human abortion and between patients with abortion precedence and education. Conclusions: Based on our study, plcA and hlyA played a key role in the virulence determination of L. monocytogenes. Data analysis also showed that L. monocytogenes could be a causative agent of abortion in pregnant women.

show abstract

Section: Discussionmentioning

confidence: 97%

Identification of Listeria monocytogenesVirulence Factors in Women With Abortion by Polymerase Chain Reaction

Eslami¹,

Goudarzi²,

Ohadi³

et al. 2014

Arch Clin Infect Dis

View full text Add to dashboard Cite

show abstract

“…19 These risks are of particular concerns in relation to 'ready-to-eat' foods, which will not receive any further processing before consumption. 25 A study by Chao et al 10 revealed that counts of Listeria were 13.4 % higher on delicatessen foods than on cooked foods investigated during their study. Moreover, non-spore-forming bacteria might be able to withstand dry conditions on surfaces for an extensive period.…”

Section: *Corresponding Authormentioning

confidence: 85%

Efficacy of low-pressure foam cleaning compared to conventional cleaning methods in the removal of bacteria from surfaces associated with convenience food

Aa¹,

Human²,

Doughari³

et al. 2014

Afr H. Sci.

View full text Add to dashboard Cite

Background: Food borne illnesses and food poisoning are cause for concern globally. The diseases are often caused by food contamination with pathogenic bacteria due largely to poor sanitary habits or storage conditions. Objectives: Prevalence of some bacteria on cleaned and sanitised food contact surfaces from eight convenience food plants in Gauteng (South Africa) was investigated with the view to evaluate the efficacy of the cleaning methods used with such food contact surfaces. Methods: The microbial load of eight convenience food manufacturing plants was determined by sampling stainless steel food contact surfaces after they had been cleaned and sanitised at the end of a day's shift. Samples were analysed for Total Plate Count (TPC), Escherichia coli, Salmonella species, Staphylococcus aureus and Listeria species. Results: Results showed that 59 % of the total areas sampled for TPC failed to comply with the legal requirements for surfaces, according to the Foodstuffs, Cosmetics and Disinfectants Act (< 100 cfu.cm -2 ). S. aureus and Salmonella were not detected, but Listeria was detected in 23 % and E. coli in 1.3 % of the samples. Fifty percent (50 %) of the plants applied conventional cleaning methods for cleaning and sanitation and 50 % used the low-pressure foam (LPF) method. There was significant difference (P ≤ 0.05) between the mean TPC values of the conventional cleaning method (14 358.82) compared to that of LPF method (2 386.51) but no significant difference (P > 0.05) in terms of Listeria species isolates obtained from both cleaning methods. The LPF method proved to be the superior cleaning option for lowering TPC counts. Conclusion: Regardless of cleaning method used, pathogens continued to flourish on various surfaces, including dry stainless steel, posing a contamination hazard for a considerable period depending on the contamination level and type of pathogen. Intensive training for proper chemical usage and strict procedural compliance among workers for efficient cleaning procedures is recommended.

show abstract

“…Listeria monocytogenes is a ubiquitous bacterium that is responsible for food-borne illnesses in humans. It has been recovered in several countries and from many different foods of animal origin that have been linked to both sporadic cases and outbreaks of listeriosis (Thévenot et al, 2006;Chao et al, 2006). Disease incidence in humans is generally low, but in recent years great attention has been addressed to study the virulence properties of this microorganism due to the severity of the illness and the high mortality rate (20-30%) (Vazquez-Boland et al, 2001;Dussurget et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Isolation of <i>Listeria monocytogenes</i> recovered from some ready-to-eat foods sold in Kano, north-western Nigeria

Aisha¹,

Kawo²

2015

Bayero J. Pure App. Sci.

View full text Add to dashboard Cite

Listeria monocytogenes and other Listeria species were isolated from some ready-to-eat foods sold in Kano metropolis. The method of United States Food and Drug Administration, Center for Food Safety and Applied Nutrition (FDACFSAN) was adopted in the isolation of the organism. A total of 336 food samples were screened. This included 29 beef burger, 30 yoghurt, 32 locally fermented fresh milk (nono), 34 cabbage, 45 grilled meat (tsire), 48 lettuce, 55 meat-pie and 63 roasted meat (balangu) samples. Samples were collected from December 2012 to March 2013. From these samples 38 (11.3%) yielded Listeria species. Six isolates (1.8%) were found to be L. monocytogenes, 31 (9.2%) were found to be L. ivanovii and 1(0.3%) was L. seeligeri. Out of the 38 isolates, 7 (18%) were from 'balangu', 9 (24%) cabbage, 14 (37%) lettuce, 5 (13%) meat pie and 3 (8%) 'tsire' samples. L. monocytogenes was isolated from 3 lettuce samples (50%), 2 'balangu' samples (33%) and 1 meat-pie sample (17%). On the other hand, L. monocytogenes was not isolated from 'tsire', beef burger, 'nono' and yoghourt samples. This study showed the presence of the organism in Kano, which could pose a threat of outbreak or sporadic cases of listeriosis with high morbidity and mortality rates.

show abstract

Prevalence of Listeria monocytogenes in delicatessen food products in China

Cited by 13 publications

References 17 publications

Identification of Listeria monocytogenesVirulence Factors in Women With Abortion by Polymerase Chain Reaction

Identification of Listeria monocytogenesVirulence Factors in Women With Abortion by Polymerase Chain Reaction

Efficacy of low-pressure foam cleaning compared to conventional cleaning methods in the removal of bacteria from surfaces associated with convenience food

Isolation of <i>Listeria monocytogenes</i> recovered from some ready-to-eat foods sold in Kano, north-western Nigeria

Contact Info

Product

Resources

About