Prevalence of Listeria species in camel sausages from retail markets in Aydin province in Turkey and RAPD analysis of Listeria monocytogenesisolates

Ozbey, Gokben; Ertaş, Hasan; Kök, Filiz

doi:10.1186/2046-0481-59-6-342

Cited by 14 publications

(11 citation statements)

References 13 publications

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“…The organism was detected in camel sausages obtained from a retail market (Ozbey et al . ), and this could be attributed to postharvest contamination. One plausible explanation for the rare occurrence of L. monocytogenes in camels could be explained by feeding behaviour.…”

Section: Discussionmentioning

confidence: 99%

The risk of carriage of Salmonella spp. and Listeria monocytogenes in food animals in dynamic populations

Stipetić

Chang

Peters

et al. 2016

Veterinary Medicine & Sci

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Salmonella spp. and Listeria monocytogenes are foodborne pathogens of global importance. We assessed their risks and associated factors in a highly dynamic population of animals. Animal and environmental samples were collected from dairy cattle, sheep, camel and chickens at either the farms or the abattoirs. The pathogens were detected using a combination of bacterial enrichment culture and real‐time polymerase chain reaction (PCR). Data on putative risk factors were collect and analysed for their significance of association with these pathogens. Salmonella spp. were detected at higher proportions in sheep faeces and sheep carcasses in comparison to cattle faeces (odds ratio = 2.4 and 2.2, respectively). This pathogen was less common in milk or carcasses samples from cattle or chickens. Sheep and camel carcass samples were highly contaminated with Salmonella spp. Faecal samples from cattle had the most diverse serovars of Salmonella enterica including S. Newport, S. Haifa, S. Kedougou, S. Kentucky, S. Mbandaka and S. Goettingen. Exotic serovars in sheep included S. Eastbourne, S. Chester and S. Kottnus. Serovars that were shed in camel faeces included S. Newport, S. Bovismorbificans and S. Infantis. In all sampled populations, detection of Salmonella spp. was more likely during warmer months than cold months. Listeria monocytogenes was not common in the targeted populations and was detected at a rate of 2.4%, mainly from sheep carcasses. The study highlights the role of food animals as reservoirs of pathogens across boundaries since all feed are imported in that population from different parts of the world.

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Section: Discussionmentioning

confidence: 99%

The risk of carriage of Salmonella spp. and Listeria monocytogenes in food animals in dynamic populations

Stipetić

Chang

Peters

et al. 2016

Veterinary Medicine & Sci

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“…Determination of genetic relatedness of L. monocytogenes strains isolated from milk. All L. monocytogenes isolates were genotyped using Random Amplification of Polymorphic DNA (RAPD) with the nonspecific primer OPA-11 (5'-CA AT CG CC GT-3') (Ozbey et al 2006). Reactions were carried out in a mixture of 25 µl, containing: 1 × PCR buffer with 2.0 mM MgCl 2 (Promega, United States), 1.5 mM MgCl 2 (ABO, Poland), 200 µM dNTPs (Promega, United States), 1.0 µM primer OPA-11 (Oligo.pl, Poland), 1.25 U Taq DNA polymerase (Promega, United States), ultrapure water (Merck, Poland), and 3.0 µl DNA.…”

Section: Methodsmentioning

confidence: 99%

Characteristics of Listeria monocytogenes Strains Isolated from Milk and Humans and the Possibility of Milk-Borne Strains Transmission

Skowron

Wałecka-Zacharksa

Grudlewska

et al. 2019

Polish Journal of Microbiology

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Listeria monocytogenes is the etiological factor of listeriosis. The main source of these organisms is food, including dairy products. The aim was to determine the multiple correlations between the drug susceptibility, virulence genes (VGs), and biofilm formation on silicone teat cups of milk-borne and human L. monocytogenes strains. The spread of L. monocytogenes via contaminated teat rubbers was assessed. The L. monocytogenes strains recovered from milk (18), human blood (10), and the reference strain ATCC®19111™ were used in the study. Penicillin resistance was the most prevalent resistance in the milk isolates (n = 8; 44.4%), whereas among clinical strains erythromycin resistance was predominating – (n = 6; 60%). The most frequent VGs among strains isolated from milk were hlyA (100%) and plcB (100%) whereas in strains isolated from blood – hlyA (100%) and prfA (90%). All tested VGs were present in 50% of blood isolates and 11% of milk-borne strains. The strains isolated from milk formed a significantly stronger biofilm. The strains with more numerous virulence genes were resistant to more antibiotics and formed a stronger biofilm. It was shown that contaminated teat cups might contribute to the transmission of L. monocytogenes in the herd. It seems reasonable to monitor the occurrence of L. monocytogenes biofilm in a dairy processing environment.

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“…Furthermore, most cattle and sheep farms in Turkey does not have adequate hygiene precautions and animals live in a natural environment together with people. Ozbey et al, 2006 aimed to study was determine the occurrence of Listeria spp. in food, the environment, in animals and to observe the relationship between these parameters in east.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and antimicrobial resistance of Listeria monocytogenes isolated from raw milk and dairy products

Gohar¹,

Abbas²,

sajid³

et al. 2017

Matrix sci. medica

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Listeriosis is a severe foodborne disease commonly caused by eating contaminated food with the Listeria species. A large variety of foods, especially dairy foods and ready-to-eat products, can support the growth of pathogens. Outbreaks of listeriosis have been related with milk, cheese, vegetable salads, and meat products, and fatality rates are typically around 20% due to listeriosis. The Centers for Disease Control and Prevention evaluates that 2500 infections and more than 500 deaths are related with listeriosis each year in the United States. A total 125 milk and dairy products were included in the study. Isolation and identification of this specie was done and then confirmed it by gram staining. Antimicrobial sensitivity was also checked. Prevalence of Listeria species were 16.8%, Listeria monocytogenes was 13.6%. Listeria monocytogenes was resistant against Ampicillin, Amoxicillin, Penicillin and sensitive Fosfomycin, Ciprofloxacin, Gentamycin. The results of this study showed the low prevalence of Listeria monocytogenes.

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Prevalence of Listeria species in camel sausages from retail markets in Aydin province in Turkey and RAPD analysis of Listeria monocytogenesisolates

Cited by 14 publications

References 13 publications

The risk of carriage of Salmonella spp. and Listeria monocytogenes in food animals in dynamic populations

The risk of carriage of Salmonella spp. and Listeria monocytogenes in food animals in dynamic populations

Characteristics of Listeria monocytogenes Strains Isolated from Milk and Humans and the Possibility of Milk-Borne Strains Transmission

Prevalence and antimicrobial resistance of Listeria monocytogenes isolated from raw milk and dairy products

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