Prevalence of &lt;I&gt;Candidatus Rickettsia tarasevichiae&lt;/I&gt;-Like Bacteria in Ixodid Ticks at 13 Sites on the Chinese-Russian Border

Sun, Yi; Hongrong, Jiang; Cao, Wu‐Chun; Fu, Weiming; Ju, Wendong; Wang, Xin

doi:10.1603/me13189

Cited by 12 publications

(8 citation statements)

References 16 publications

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“…Ixodes persulcatus, a tick species distributed widely from the eastern Baltic coast to Japan (22)(23)(24)(25), has been reported to harbour symbionts including the Montezuma agent, now called Candidatus Lariskella arthropodarum (26)(27)(28), Coxiella and Spiroplasma spp. (29), as well as human and livestock pathogens including tick-borne encephalitis virus (TBEV), Kemerovo virus, Alongshan virus, Anaplasma phagocytophilum, Candidatus Neoehrlichia mikurensis, Ehrlichia muris, Rickettsia helvetica, Rickettsia heilongjiangensis, Candidatus Rickettsia tarasevichiae, Borrelia miyamotoi, Borrelia burgdorferi sensu lato, Theileria equi and several species of Babesia (25,(30)(31)(32)(33)(34)(35)(36)(37)(38). Co-infections with multiple pathogens and symbionts are common (29,35,39).…”

Section: Introductionmentioning

confidence: 99%

Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks

et al. 2021

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Spiroplasma are vertically-transmitted endosymbionts of ticks and other arthropods. Field-collected Ixodes persulcatus have been reported to harbour Spiroplasma, but nothing is known about their persistence during laboratory colonisation of this tick species. We successfully isolated Spiroplasma from internal organs of 6/10 unfed adult ticks, belonging to the third generation of an I. persulcatus laboratory colony, into tick cell culture. We screened a further 51 adult male and female ticks from the same colony for presence of Spiroplasma by genus-specific PCR amplification of fragments of the 16S rRNA and rpoB genes; 100% of these ticks were infected and the 16S rRNA sequence showed 99.8% similarity to that of a previously-published Spiroplasma isolated from field-collected I. persulcatus. Our study shows that Spiroplasma endosymbionts persist at high prevalence in colonised I. persulcatus through at least three generations, and confirms the usefulness of tick cell lines for isolation and cultivation of this bacterium.

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Section: Introductionmentioning

confidence: 99%

Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks

et al. 2021

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“…R . tarasevichiae was first reported in northeastern China in 2012 [ 35 ] and is widely distributed in eastern and northeastern China [ 11 , 36 , 37 ]. The forest region in northeast China is close to Russia in terms of geography.…”

Section: Discussionmentioning

confidence: 99%

“…C. R. tarasevichiae was first identified in I. persulcatus ticks from various sites in Russia in 2001 [34]. Human infection with C. R. tarasevichiae was first reported in northeastern China in 2012 [35] and is widely distributed in eastern and northeastern China [11,36,37]. The forest region in northeast China is close to Russia in terms of geography.…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Co-infection of tick-borne bacterial pathogens in ticks in Inner Mongolia, China

et al. 2023

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Tick-borne infectious diseases pose a serious health threat in certain regions of the world. Emerging infectious diseases caused by novel tick-borne pathogens have been reported that are causing particular concern. Several tick-borne diseases often coexist in the same foci, and a single vector tick can transmit two or more pathogens at the same time, which greatly increases the probability of co-infection in host animals and humans and can lead to an epidemic of tick-borne disease. The lack of epidemiological data and information on the specific clinical symptoms related to co-infection with tick-borne pathogens means that it is not currently possible to accurately and rapidly distinguish between a single pathogen infection and co-infection with multiple pathogens, which can have serious consequences. Inner Mongolia in the north of China is endemic for tick-borne infectious diseases, especially in the eastern forest region. Previous studies have found that more than 10% of co-infections were in host-seeking ticks. However, the lack of data on the specific types of co-infection with pathogens makes clinical treatment difficult. In our study, we present data on the co-infection types and the differences in co-infection among different ecological regions through genetic analysis of tick samples collected throughout Inner Mongolia. Our findings may aid clinicians in the diagnosis of concomitant tick-borne infectious diseases.

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“…R. jingxinensis”, were detected in ticks, with an overall infection rate of 6.12 %. Previous reports showed that Rickettsia infection rates ranged from 1.53 to 32.25 % in a certain species, or a certain vector, or a certain origin of northeastern China [ 21 , 34 ]. Intriguingly, the infection rates of rickettsiae in Heilongjiang Province were found to be strikingly higher than those of Jilin Province in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…“ Ca. R. tarasevichiae” was recently found in patients and I. persulcatus in Heilongjiang Province in China, renewing the old concept that members of the ancestral group of Rickettsia were nonpathogenic [ 8 , 34 ]. In this study, the presence of “ Ca.…”

Section: Discussionmentioning

confidence: 99%

Characterization of rickettsiae in ticks in northeastern China

Liu

Zhang

et al. 2016

Parasites Vectors

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BackgroundTick-borne rickettsioses are considered important emerging zoonoses worldwide, but their etiological agents, rickettsiae, remain poorly characterized in northeastern China, where many human cases have been reported during the past several years. Here, we determined the characteristics of Rickettsia spp. infections in ticks in this area.MethodsTicks were collected by flagging vegetation from Jilin and Heilongjiang provinces of northeastern China followed by morphological identification. The presence of Rickettsia spp. in ticks was detected by PCR targeting the 23S-5S ribosomal RNA intergenic spacer, citrate synthase (gltA) gene, and 190-kDa outer membrane protein gene (ompA). The newly-generated sequences were subjected to phylogenetic analysis using the software MEGA 6.0.ResultsThe overall infection rate of Rickettsia spp. was 6.12 %. Phylogenetic analyses based on the partial gltA and ompA genes demonstrated that rickettsiae detected in the ticks belong to four species, including “Candidatus Rickettsia tarasevichiae”, Rickettsia heilongjiangensis, Rickettsia raoultii, and a potential new species isolate. The associated tick species were also identified, i.e. Dermacentor nuttalli and Dermacentor silvarum for R. raoultii, Haemaphysalis concinna and Haemaphysalis longicornis for R. heilongjiangensis, and Ixodes persulcatus for “Ca. R. tarasevichiae”. All Rickettsia spp. showed significantly high infection rates in ticks from Heilongjiang when compared to Jilin Province.ConclusionRickettsia heilongjiangensis, R. raoultii and “Ca. R. tarasevichiae” are widely present in the associated ticks in northeastern China, but more prevalent in Heilongjiang Province. The data of this study increase the information on the distribution of Rickettsia spp. in northeastern China, which have important public health implications in consideration of their recent association with human diseases.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1764-2) contains supplementary material, which is available to authorized users.

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Prevalence of <I>Candidatus Rickettsia tarasevichiae</I>-Like Bacteria in Ixodid Ticks at 13 Sites on the Chinese-Russian Border

Cited by 12 publications

References 16 publications

Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks

Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks

Co-infection of tick-borne bacterial pathogens in ticks in Inner Mongolia, China

Characterization of rickettsiae in ticks in northeastern China

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