Prevalence of &lt;i&gt;Listeria&lt;/i&gt; species in some foods and their rapid identification

Yehia, Hany M.; Ibraheim, Shimaa M.; Hassanein, Wesam A.

doi:10.4314/tjpr.v15i5.21

Cited by 15 publications

(22 citation statements)

References 15 publications

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“…were less frequent [32,33]. Yehia et al [34] found that 50% of raw beef and chicken samples and 30% of raw fish samples contained listeria. In addition, Listeria spp.…”

Section: The Incidence Of Listeria Spp In Different Samplesmentioning

confidence: 99%

Prevalence and antibiotic resistance of Listeria monocytogenes in camel meat

et al. 2020

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In the present study, a total of 50 raw camel meat samples were analyzed for the presence of Listeria monocytogenes. The isolates were characterized via morphological and culture analyses; identification of isolates was confirmed by polymerase chain reaction (PCR) and sequencing of the listeriolysin O gene. The API Listeria system was used for further chemical identification and verification of the strains. L. monocytogenes was identified in eight raw camel meat samples, which was the highest incidence (16%) of contamination, followed by L. seeligeri 3(6%), L. innocua and L. welshimeri 2 (2% each), and L. grayi 1 (1%). According to Basic Local Alignment Search Tool (BLAST) analysis, isolated strains that were positive for the listeriolysin O gene were >99% similar to the published database sequences for L. monocytogenes strain LM850658 (sequence ID: CP009242.1). We studied the antibiotic resistance profile of the L. monocytogenes strains with common antibiotics used to treat human listeriosis and demonstrated that almost all strains tested were susceptible to the antibiotics.

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“…were less frequent [32,33]. Yehia et al [34] found that 50% of raw beef and chicken samples and 30% of raw fish samples contained listeria. In addition, Listeria spp.…”

Section: The Incidence Of Listeria Spp In Different Samplesmentioning

confidence: 99%

Prevalence and antibiotic resistance of Listeria monocytogenes in camel meat

et al. 2020

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“…These products often include beef and poultry products such as deli meats, frankfurters, canned meats (tuna, chicken, and spam), as well as beef and another meat jerky (Seman et al, 2018). Due to the nature of RTE foods, there is an outstanding concern for the consumption of contaminated food (Yehia et al, 2016). One of the leading foodborne pathogens associated with RTE foods is Listeria monocytogenes (McLauchlin et al, 2004;Zhu et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…One of the leading foodborne pathogens associated with RTE foods is Listeria monocytogenes (McLauchlin et al, 2004;Zhu et al, 2005). L. monocytogenes is ubiquitous in nature and able to grow in both aerobic and anaerobic environments, enabling it to persevere in several different conditions (Saha et al, 2015;Yehia et al, 2016). In addition, L. monocytogenes acts as a saprophytic organism with the capability to adapt to an ever-changing environment because it possess multiple stress response mechanisms such as overcoming varying temperatures (0-45 • C), salt concentrations (up to 10% NaCl) and is non-extremophilic (pH 4.5-9.2) (Ryser et al, 1997;Ryser and Marth, 2007;Wang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Listeriosis has been reported to have a mortality rate of 19-30% in immunocompromised patients in the United States (Swaminathan and Gerner-Smidt, 2007;Goulet et al, 2012; Center for Disease Control Prevention (CDC), 2019). Most listeriosis cases are caused by transmission through fresh produce and RTE food (Yehia et al, 2016;Zhu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Application of an Alternative Inorganic Acid Antimicrobial for Controlling Listeria monocytogenes in Frankfurters

Bodie

Dittoe

Feye

et al. 2019

Front. Sustain. Food Syst.

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The prevalence of Listeria monocytogenes under refrigerated conditions is a concern for ready to eat meats like Frankfurters. The purpose of this study was to evaluate the bactericidal effect of potential antimicrobials, Bisulfate of Soda (SBS), nisin, and their combination on L. monocytogenes in frankfurters. Antimicrobial treatment concentrations of SBS and nisin were indicated by performing minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) test. Results of the MIC indicated the concentrations 0.75 and 1.5% SBS and 0.5, 1, and 2% nisin. Frankfurters were inoculated with L. monocytogenes (initial population: 6.65 log CFU/mL and treated with water, SBS (0.75 and 1.5%) nisin (0.5, 1, and 2%) and combinations (0.75% SBS+0.5% nisin, 0.75% SBS+1% nisin, 1.5% SBS+1% nisin, and 1.5% SBS+2% nisin). After treatment, frankfurters were removed, rinsed in neutralizing buffer, and plated on Oxford agar. Data were analyzed using One-Way ANOVA, linear contrasts and pairwise comparisons in JMP 14.0. Differences were determined using Tukey's protected HSD and considered significant when P ≤ 0.05. The treatment 1.5% SBS + 2% nisin exhibited the greatest reduction of L. monocytogenes (2.66 log CFU/mL; P < 0.001). Although the effectiveness of both SBS and nisin concentrations were similar when compared to (0.75% SBS-2.19 log CFU/mL; 1.5% SBS-2.29 log CFU/mL; 5% nisin-1.99 log CFU/mL; 1% nisin-2.47 log CFU/mL; 2% nisin-2.42 log CFU/mL), there was a linear effect shown by the SBS and nisin treatments compared to the tap water treatment (P < 0.0001; P < 0.0001). The effectiveness of 0.75% SBS compared to other treatments using Pairwise comparisons determined that frankfurters treated with 0.75% SBS and 0.75% SBS+1% nisin resulted in lower L. monocytogenes populations than tap water (TW), whereas 0.75% SBS+0.5% nisin was not different (P < 0.0001). Furthermore, when evaluating the efficacy of 1.5% SBS using Pairwise comparisons, 1.5% SBS, and 1.5% SBS combinations were all significantly different than TW (P < 0.0001). Together, both antimicrobials can reduce Listeria without diminishing the appearance of the frankfurter. The results suggest incorporating SBS and nisin as an alternative antimicrobials for frankfurters can be effective for decreasing L. monocytogenes.

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“…It is transmitted to infect the susceptible individuals via consumption of the contaminated foods. The major risk population groups at risk for invasive listeriosis are the immunocompromised hosts such as pregnant women, unborn or newly delivered infants, organ transplant recipients, cancer and AIDS patients, and the elderly, with fatality rates of 20-30% (Jamali et al, 2013;Yehia et al, 2016;Phraephaisarn et al, 2017). Listeria species are widely distributed in many different environments.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Characterization of Listeria Species from Raw Milk and Dairy Products from Çanakkale Province

Şanlibaba

Tezel

2018

Turkish JAF Sci.Tech.

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The objective of this study was to determine the prevalence of Listeria species, specifically Listeria monocytogenes, in raw milk, pasteurized milk, white cheese, and homemade cheese. A total of 200 food samples were collected and analyzed to examine the presence of Listeria spp. The EN ISO 11290-1 method was used for isolation of Listeria. API Listeria test kit was used for biochemically characterization. Listeria spp. were isolated in 25 of the 200 samples (12.5%). The largest number of Listeria spp. was detected in homemade cheese (24%), followed by raw milk (18%), and white cheese (8%). Listeria spp. were not isolated from the pasteurized milk. The most common species isolated were Listeria innocua (5.5%); the remaining Listeria isolates were Listeria ivanovi (3.5%), Listeria welshimeri (3%), and Listeria monocytogenes (0.5%). Listeria monocytogenes was detected in only raw milk.

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Prevalence of <i>Listeria</i> species in some foods and their rapid identification

Cited by 15 publications

References 15 publications

Prevalence and antibiotic resistance of Listeria monocytogenes in camel meat

Prevalence and antibiotic resistance of Listeria monocytogenes in camel meat

Application of an Alternative Inorganic Acid Antimicrobial for Controlling Listeria monocytogenes in Frankfurters

Prevalence and Characterization of Listeria Species from Raw Milk and Dairy Products from Çanakkale Province

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