Prevalence of mucosal types of human papillomavirus in skin lesions in north part of Iran

Shahmahmoudi, Shohreh; Mahmoodi, Mahmood; Azad, Talat Mokhtari; Rad, K. Samimi; Tabatabaie, Hamideh; Sarijlou, M; Pour, Yousef Yahya; Yousefi, Maryam; Ghasemi, Maryam; Far, Khalil Jahani; Nategh, R

doi:10.1016/j.canlet.2006.03.023

Cited by 14 publications

(24 citation statements)

References 25 publications

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“…Following our previous study on detection of mucosal HPVs in non-melanoma skin cancer (Shahmahmoudi et al, 2007), the aim of the present survey was to investigate the presence of HPV-DNA and determination of HPV type in breast tissues in a group of Iranian women with and without breast cancer. Paraffin-embedded specimens from 65 malignant breast cancer cases and 65 cases with benign breast lesions were investigated for presence of HPV-DNA by nested-polymerase chain reaction.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that nested PCR using MY09/11 and GP5+/6+ as outer and inner primers, respectively, is much more sensitive than solely MY or GP primers in detecting mucosal HPV types (Husnjak et al, 2000). In our previous study (Shahmahmoudi et al, 2007), we detected mucosal types of HPV in skin lesions by using the above mentioned primers; so we chose to use the same nested PCR protocol for breast tissues as well.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, other authors failed to demonstrate the presence of HPV DNA in samples of breast carcinoma, even using the same methods of detection (Gopalkrishna et al, 1996;Hachana et al, 2010). Considering these controversial data and following our previous study (Shahmahmoudi et al, 2007), our aim was to investigate the presence of HPV-DNA of high and low-risk mucosal types in female breast carcinomas as well as in non-cancerous breast tissues of a group of Iranian women by nested PCR.…”

Section: Introductionmentioning

confidence: 92%

“…Nested-PCR was performed according to protocols mentioned in previous studies (Shahmahmoudi et al, 2007).…”

Section: Dna Extraction and Hpv Detectionmentioning

confidence: 99%

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No Detection of 'High-risk' Human Papillomaviruses in a Group of Iranian Women with Breast Cancer

Ahangar-Oskouee¹,

Shahmahmoodi²,

Jalilvand³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Asian Pac J Cancer Prev, 15 (9), [4061][4062][4063][4064][4065] IntroductionBreast cancer is the second most common cancer in the world, accounts for 25% of all cancers (excluding nonmelanoma skin cancers) in women (GLOBOCAN, 2012). According to recently published data, the overall cancer incidence in Iran has been 110 per 100,000 in men and 98 per 100,000 in women in 2006. Among Iranian women, breast cancer is the leading cause of cancer morbidity and mortality, even though its incidence is among the lowest in the world, i.e., 25 per 100,000 (Mousavi et al., 2008).Breast cancer affects Iranian women at least one decade younger than their counterparts in developed countries (Harirchi et al., 2004). There are many published studies about breast cancer in Iran, but the epidemiological aspects of Iranian breast cancer are uncertain.Well known risk factors of breast cancer are genetic and epigenetic factors including: mutations in breast cancer susceptibility genes BRCA1 and BRCA2, TP53, sex-steroid hormones and lifestyle factors (Hulka and Moorman, 2008;Derks-Smeets et al., 2014).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

“…Nested-PCR was performed according to protocols mentioned in previous studies (Shahmahmoudi et al, 2007).…”

Section: Dna Extraction and Hpv Detectionmentioning

confidence: 99%

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No Detection of 'High-risk' Human Papillomaviruses in a Group of Iranian Women with Breast Cancer

Ahangar-Oskouee¹,

Shahmahmoodi²,

Jalilvand³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…The PM-PCR RHA method has previously been described in detail. 24,26 The method was designed for the identification of 25 established beta-PV types, namely genotypes 5,8,9,12,14,15,17,19,20,21,22,23,24,25,36,37,38,47,49,75,76,80,92,93 and 96.…”

Section: Dna Isolation and Pcr Reactions For Mutation Analysesmentioning

confidence: 99%

CDKN2A but not TP53 mutations nor HPV presence predict poor outcome in metastatic squamous cell carcinoma of the skin

Küsters‐Vandevelde

Leeuwen

Verdijk

et al. 2009

Intl Journal of Cancer

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Genetic alterations in metastatic cutaneous squamous cell carcinoma (CSCC) which might serve as prognostic biomarkers are not well investigated. We investigated the mutation status and protein expression of the CDKN2A (INK4a-ARF) and TP53 genes in metastatic CSCCs and correlated this with clinicopathological variables, HPV presence, and survival data. Sequence analysis was performed on formalin-fixed and paraffin-embedded tissue of 35 metastases and their primary tumors, and was correlated with immunohistochemical stainings for p53, p16 and p14. Beta-PV and alpha-PV DNA was detected using PCRbased assays. Kaplan-Meier and Cox regression methods were used for survival assessment. CDKN2A was mutated in 31% of the metastases and their primary tumors, while the TP53 gene was mutated in 51% of the metastases. P53 protein expression was significantly associated with missense type of mutations (p 5 0.002). No persistent HPV types were detected. CDKN2A mutations were significantly associated with disease-specific death (p 5 0.001). A significant difference was observed in disease-specific survival between patients with or without a CDKN2A mutation (p 5 0.010), while this was not the case for TP53. At univariate Cox's regression analysis tumor size (p 5 0.010), invasion depth (p 5 0.030) and CDKN2A mutations (p 5 0.040) were significantly related to shorter disease-specific survival. At multivariate Cox's regression only tumor size had an adverse effect on survival (p 5 0.002). In conclusion, our study indicates that the CDKN2A mutation status might be of prognostic value in metastatic CSCCs. In most cases, CDKN2A and TP53 mutations are early genetic events in CSCC tumorigenesis. The possible role of HPV in metastatic CSCC needs further exploration.Cutaneous squamous cell carcinoma (CSCC) is the second most common malignancy in Caucasians, after basal cell carcinoma, and its incidence is still rising. 1 Generally the risk of metastasis is reported up to 5%, while immunocompromised patients have a larger tendency to metastasize (5-10%). 1,2 The outcome of these patients once metastasized is poor, with a 5-year survival rate between 25 and 50%. 3,4 Genetic alterations in CSCCs which might serve as prognostic biomarkers are not well investigated.TP53 mutations are the most frequent genetic alterations in CSCC with incidences of up to 50%. 5,6 TP53 mutations are early events in skin carcinogenesis, with a similar mutation frequency of 50% in actinic keratosis, a precancerous skin lesion that potentially can progress to CSCC. 7 Mutations in the CDKN2A (INK4a-ARF) locus are reported in CSCC with frequencies ranging up to 24% in sporadic CSCC. [8][9][10] This gene maps to chromosome 9p21, which by alternative transcripts encodes for 2 cell cycle regulatory proteins, p16 INK4a (Exons 1a, 2 and 3) and p14 ARF (Exons 1b, 2 and 3). 10,11 The exact role of the CDKN2A gene in skin carcinogenesis is not well understood. Especially in metastatic CSCCs alterations in the CDKN2A gene have not been investigated. In a small previous stud...

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Detection and genotyping of human papillomaviruses and their role in the development of ovarian carcinomas

Mališić

Janković

Jakovljevic

2012

Arch Gynecol Obstet

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Prevalence of mucosal types of human papillomavirus in skin lesions in north part of Iran

Cited by 14 publications

References 25 publications

No Detection of 'High-risk' Human Papillomaviruses in a Group of Iranian Women with Breast Cancer

No Detection of 'High-risk' Human Papillomaviruses in a Group of Iranian Women with Breast Cancer

CDKN2A but not TP53 mutations nor HPV presence predict poor outcome in metastatic squamous cell carcinoma of the skin

Detection and genotyping of human papillomaviruses and their role in the development of ovarian carcinomas

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