Prevalence of multidrug resistance bacterial isolates from infected wound patients in Dhaka, Bangladesh: A cross-sectional study

Abstract

Introduction: Multidrug resistance (MDR) is threatening the adequate coverage of antibiotics. This study aimed to analyze the antimicrobial resistance pattern of bacterial isolates from wound infection and the scenario of multidrug resistance. Methods: Microbiological culture results scripts of Medinova Medical Services LTD, representing non-repetitive various wound samples (post-surgical, trauma, superficial skin, burn) reported between January 2017 and March 2018, were retrieved and analyzed for pathogens and their antimicrobial resistance patterns using R version 3.5.3. Results: Overall, 1266 bacterial isolates were obtained, and 850 (67.1%) were identified as MDR. The percentage of MDR among gram-positive and gram-negative bacterial isolates were 68.8% and 66.0%, respectively. Among isolates Staphylococcus aureus (n = 401), Pseudomonas aeruginosa (n = 200), and Escherichia coli (n = 193) were predominant. Vancomycin followed linezolid showed most activity against gram-positive bacteria. Whereas, Colistin was found to be the most active against most of the gram-negative bacterium except for the Proteus spp in sensitivity test. Although, carbapenem group was determined to be the best against Proteus spp. About 82% Enterococcus spp and 76% Proteus spp were MDR. Isolates from patients aged >60 years (AOR = 1.774 95%CI: 1.089–2.892) were more prone to becoming MDR in compared to other age group and was a significant determinant (P = 0.02) of MDR. Conclusions: Our study revealed that the presence of MDR pathogens in wound infection was noteworthy. The findings of this study would assist in decision making of wound infection treatment. Highlights

Antimicrobial, Antibiofilm, and Antioxidant Properties of Boletus edulis and Neoboletus luridiformis Against Multidrug-Resistant ESKAPE Pathogens

“…The efficacy of antibiotics is influenced by several factors, namely, the wound microorganisms, their antibiotic-resistance profile, and the presence of bacteria-derived biofilm, which significantly enhances their tolerance to antibiotics ( 4 ). The most notorious MDR bacteria was identified as “ESKAPE,” which includes Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa , and Enterobacter species that are among the most prevalent bacteria in cutaneous infections ( 5 ). These ESKAPE pathogens have revived the attention on mushroom extracts as promising agents with antibacterial and antibiofilm activities.…”

Microbiological Profile, Biofilm Formation and Antimicrobial Resistance Pattern of Pathogenic Bacteria Isolated from Chronic Ulcerative Lesions

“…were completely resistant to cefepime, ampicillin, and tetracycline, 53.8% of isolates were resistant to vancomycin, and 23.1% to linezolid. In another study by Alam et al, 28 Enterococcus spp. showed the least resistance to vancomycin (0%), linezolid (3.3%).…”

Detection of blaOXA-145, blaOXA-224, blaOXA-539, and blaOXA-675 Genes and Carbapenem-Hydrolyzing Class D β-Lactamases (CHDLs) in Clinical Isolates of Pseudomonas aeruginosa Collected from West of Iran, Hamadan

“…Hydrophobic drugs enter bacterial cells through the phospholipid layer, while hydrophilic drugs enter through porins in Gram-negative bacteria (GNB) [ 1 , 2 ]. Some bacterial species, such as Pseudomonas aeruginosa , have an outer membrane that is less porous than other species, causing the bacteria to be less susceptible to antimicrobial agents [ 3 , 4 ]. P. aeruginosa is the most common organism isolated among the nonfermenters from the clinical specimens.…”

“…P. aeruginosa is the most common organism isolated among the nonfermenters from the clinical specimens. Resistance to various antibacterial drugs can be acquired through mutations causing loss, reduced size, or decreased expression of outer membrane proteins (OMPs) in bacteria [ 3 , 5 ]. Infections caused by bacterial pathogens with multidrug-resistant (MDR), extremely drug-resistant (XDR), and pan drug-resistant phenotypes (PDR) are challenging and difficult to treat.…”