Prevalence of  multidrug resistance patterns of Escherichia coli from suspected urinary tract infection in  Mymensingh city, Bangladesh

Nobel, Fahim; Akter, Sharmin; Jebin, Ruksana; Sarker, Titash; Ms, Rahman; Zamane, Saad; Islam, Khairul; Sabrina, Saima; Akther, Nishat; Hasan, Mohd Shahnaz; Islam, Mohammod

doi:10.5455/jabet.2021.d126

Cited by 8 publications

(6 citation statements)

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“…Furthermore, the age range of studied UTI patients was 7–84 years, with a mean of 45.5 years, and most were females (69%). These outcomes are in line with that found by a study in Bangladesh, which reported the UTI patient’s average age was 6–87 years with a mean age of 43.87 ± 22.8, and a higher rate of infected females (77.27%) ( Hossain et al, 2020 ), while another study found that 66.2% of their studies patients with UTI were females with the mean age of 45.50 years ( Nobel et al, 2021 ).…”

Section: Discussionsupporting

confidence: 89%

“…In the current study, among 400 tested urine patients, only 42.7% were culture positive, close to the outcomes of a Bangladeshi study that detected 453 positive urine cultures among 4,000 suspected UTI patients ( Nobel et al, 2021 ). Furthermore, the age range of studied UTI patients was 7–84 years, with a mean of 45.5 years, and most were females (69%).…”

Section: Discussionsupporting

confidence: 85%

“…( Bagheri-Nesami et al, 2016 ). On the other hand, E. coli was the only uropathogenic bacterial species found in the patient’s urine culture by another group ( Nobel et al, 2021 ).…”

Section: Discussionmentioning

confidence: 98%

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Antimicrobial susceptibility and integrons detection among extended-spectrum β-lactamase producing Enterobacteriaceae isolates in patients with urinary tract infection

Abubaker¹,

Anwar

2023

PeerJ

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Background Integrons are bacterial mobile genetic components responsible for mediating the antibiotic resistance process by carrying and spreading antimicrobial resistance genes among bacteria through horizontal gene transfer. Objectives This cross-sectional hospital-based study aimed to find the prevalence of antibiotic resistance patterns and to detect integrons classes (I, II, and III) among bacterial isolates in patients with urinary tract infections (UTI) in Sulaimani, Iraq. Patients and Methods Mid-stream urine samples (no. = 400) were collected from patients with UTI at three different Hospitals from Sulaimani, Iraq, between September 2021 to January 2022. Urine samples were cultured on various agar media, and grown bacteria were isolated. Antibiotic susceptibility test (AST) and an extended-spectrum β-lactamase (ESBL) screen were done for isolated bacteria. Then, integrons classes were screened using conventional PCR with gene sequencing and uploaded to the National Center for Biotechnology Information (NCBI). Results The frequency rate of Enterobacteriaceae was 67.03% among positive urine cultures. E. coli (no. = 86) and Klebsiella pneumoniae (no. = 32) isolates were identified. The most sensitive antibiotics were the carbapenem group (85.3%) and nitrofurantoin (NFN) (64.2%), while the most resistant antibiotics were nalidixic acid (NA) and 3rd generation cephalosporin. The occurrence rate of ESBL was 56.6% with a predominance of class I integron (54.2%), then class II (15.8%) and no positive record for class III integron were observed. Conclusion Most bacterial isolates from patients with UTI produced class I and II integrons genes with favourable ESBL properties.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 85%

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Antimicrobial susceptibility and integrons detection among extended-spectrum β-lactamase producing Enterobacteriaceae isolates in patients with urinary tract infection

Abubaker¹,

Anwar

2023

PeerJ

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“…The selection of appropriate antibiotics is crucial for the treatment of infection and therefore analysis of the bacterial susceptibility pattern is helpful in this context.The objective of our study was to analyze antibiotic susceptibility pattern of [12][13][14][15], India (susceptibile 50% to 80%) [16], and Nepal (susceptibile 87.8% to 95.3%) [17] also reported carbapenem as the most effective antibiotic against Enterobacteriaceae.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibility patterns of bacterial isolates from routine clinical specimens of a tertiary hospital in Bangladesh

Hossain

Begum

2021

IMC Journal of Medical Science

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Background and objectives: To prevent the emergence and spreading of antimicrobial resistance, especially multidrug resistance in pathogenic bacteria, the selection of appropriate antibiotics is a prerequisite for the effective treatment of infection.This study aimed to analyze the prevalence and antimicrobial resistance patterns of bacterial isolates from various clinical samples in a tertiary care hospital. Methods: This study was conducted at a teaching hospital of Dhaka city, Bangladesh from January 2020 to March 2021. The results of culture and antimicrobial susceptibility of bacterial isolates from various clinical samples were collected and analysed. Identification of bacteria and antimicrobial susceptibility test were performed according to the standard methods. Results: A total of 1277 bacterial isolates was analyzed. Of them, 1072 (83.95%) were Gram-negative, and 205 (16.05%) were Gram-positive bacteria. Among the isolates, Escherichia coli (n=576), Enterobacter spp. (n=150), Klebsiella spp. (n=140), and Staphylococcus aureus (n=117) were predominant.The Enterobacteriaceae showed higher resistance to cephradine (94.3%) and cefuroxime (76.7%), whereas least resistant to imipenem (10.1%) and meropenem (14.8%). Pseudomonas spp. was highly resistant to ceftriaxone (80.2%), and colistin (70.8%), whereas least resistant to piperacillin-tazobactam (15.1%). Colistin was the most effective agent (resistance 6.7%) against Acinetobacter spp. Linezolid (resistance 1%) and vancomycin (resistance 2%) were highly effective against Gram-positive bacteria. Among the Staphylococcus aureus, 95.7% were methicillin-resistant (MRSA). A total of 889 (69.6%) bacterial isolates were identified as multidrug resistant. Multidrug resistance was more prevalent in Gram-positive isolates (79.5%) than that of Gram-negative bacteria (67.7%). Furthermore, 7.5% of Gram-negative bacterial isolates were resistant to all seven classes of antibiotics tested. Conclusions: This study revealed presence of high rate of resistance to several antimicrobial agents in bacteria isolated from various clinical samples.The findings would help healthcare professionals to select appropriate antibiotics for the effective treatment of infections and to develop antibiotic stewardship protocol. IMC J Med Sci 2022; 16(1): 005 *Correspondence: M. Mahboob Hossain, Microbiology Program, Department of Mathematics and Natural Sciences (MNS), BRAC University, 66 Mohakhali, Dhaka 1212. E-mail: mmhossain@bracu.ac.bd

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“…Unfortunately, inappropriate and excessive use of antibiotics has led to the emergence of resistant bacteria. Among antibiotic-resistant E. coli strains, the prevalence of aminoglycosideresistant strains is high [3][4][5] . Gentamicin is an aminoglycoside antibiotic that inhibits bacterial protein synthesis by binding to 30S ribosomal component, thereby inhibiting the growth of dividing bacteria.…”

Section: Introductionmentioning

confidence: 99%

Zinc Oxide Nanoparticles: A Promising Solution for Controlling the Growth of Gentamicin-Resistant Uropathogenic Escherichia coli

Mardani

Fozouni²,

Najafpour³

2022

IEM

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Backgrounds: Uropathogenic Escherichia coli is one of the most important etiological agents of UTI. The aim of this study was to investigate the antibacterial effects of zinc oxide nanoparticles (ZnONPs) on aminoglycoside-resistant E. coli isolates from patients with UTI. Materials & Methods: After identifying E. coli strains in 100 out of 250 urine samples, antibiotic susceptibility was evaluated against six antibiotic classes (with emphasis on aminoglycosides) by disk diffusion method according to CLSI-2020 guidelines. The presence of aac (6')-Ie-aph (2'') gene in isolates was investigated by PCR. Antibacterial properties and minimum inhibitory concentration (MIC) of zinc oxide nanoparticles were evaluated by agar well diffusion and broth microdilution assays, respectively. Findings: Among 100 E. coli isolates, the highest and lowest antibiotic resistance rates were observed against tetracycline (70%) and ofloxacin (10%), respectively. Of 30 gentamicinresistant E. coli isolates, 17 (56.5%) isolates harbored the aac (6')-Ie-aph (2'') gene. In agar well diffusion assay, 22 (74%) gentamicin-resistant isolates were eliminated by zinc oxide nanoparticles at a concentration of 150 mg/L, while ZnONPs at 300 mg/L could eliminate all gentamicin-resistant isolates. Furthermore, ZnONPs could inhibit all bacteria at a concentration of 200 μg/mL (MIC 90 ≥ 100). Conclusions: Spread of the aac(6')-Ie-aph(2'') gene could increase gentamicin resistance among E. coli strains causing UTI. Given the favorable antibacterial effects of zinc oxide nanoparticles in vitro, the clinical application of these nanoparticles in the treatment of UTIs caused by multidrug-resistant E. coli could be investigated in future studies.

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Prevalence of multidrug resistance patterns of Escherichia coli from suspected urinary tract infection in Mymensingh city, Bangladesh

Cited by 8 publications

References 0 publications

Antimicrobial susceptibility and integrons detection among extended-spectrum β-lactamase producing Enterobacteriaceae isolates in patients with urinary tract infection

Antimicrobial susceptibility and integrons detection among extended-spectrum β-lactamase producing Enterobacteriaceae isolates in patients with urinary tract infection

Antimicrobial susceptibility patterns of bacterial isolates from routine clinical specimens of a tertiary hospital in Bangladesh

Zinc Oxide Nanoparticles: A Promising Solution for Controlling the Growth of Gentamicin-Resistant Uropathogenic Escherichia coli

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