Prevalence of multidrug-resistant Staphylococcus aureus isolates with strong biofilm formation ability among animal-based food in Shanghai

Ou, Chujun; Shang, Dabao; Yang, Jinming; Chen, Bo; Chang, Jiang; Jin, Fangning; Shi, Chunlei

doi:10.1016/j.foodcont.2020.107106

Cited by 46 publications

(44 citation statements)

References 22 publications

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“…Our study revealed that more chicken samples were positive for Salmonella than duck and pork samples, with no significant difference (p > 0.05), which was in agreement with the results reported by Yang et al (2010) and Ou et al (2020). This indicated that Salmonella contamination in retail meats in Anhui Province was serious, especially in chicken.…”

Section: Discussionsupporting

confidence: 91%

Occurrence and antimicrobial resistance of Salmonella isolated from retail meats in Anhui, China

Chen

Shao

et al. 2021

Food Science & Nutrition

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Section: Discussionsupporting

confidence: 91%

Occurrence and antimicrobial resistance of Salmonella isolated from retail meats in Anhui, China

Chen

Shao

et al. 2021

Food Science & Nutrition

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“…A previous report also documented that 100% MRSA isolates were MDR [ 23 ]. MDR MRSA from retail chicken meat were also reported from different parts of the world, 64.3% in Nigeria [ 6 ], 45.7% in India [ 33 ], and 44.4% in China [ 34 ]. The high occurrence of MDR MRSA and MSSA in frozen chicken meat was related with the type of brands, which might be due to brand level differences in production, handling, processing, and packaging including the type of antimicrobials used.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Multidrug Resistance Pattern of Methicillin Resistant S. aureus Isolated from Frozen Chicken Meat in Bangladesh

et al. 2021

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Infections by methicillin-resistant Staphylococcus aureus (MRSA) are continuously expanding within the community. Chicken meat is usually contaminated by MRSA, and this contaminated chicken meat is an important source of foodborne infections in humans. In this study, a cross-sectional supershop survey was conducted to determine the prevalence and antimicrobial resistance pattern of MRSA in 113 domestic frozen chicken meat samples purchased from nine branded supershops available in five divisional megacities of Bangladesh. The study also focused on the determination of methicillin resistance gene in MRSA isolates. S. aureus was identified by standard culture-based and molecular methods, and subjected to antimicrobial susceptibility testing. MRSA was screened by cefoxitin disk diffusion test. Methicillin resistance gene was identified by PCR. Of samples, 54.9% were positive for S. aureus, and, of these, 37.1% isolates were identified as MRSA. All the isolates were multidrug resistant (MDR): 52.2% were resistant to 6–8 antimicrobial classes, and 47.8% isolates to 9–12 classes. Three (3.2%) isolates of S. aureus were possible extensively drug resistant. The highest rates of resistance were observed against cefoxitin (100%), followed by nalidixic acid, ampicillin and oxacillin (97.7%), colistin (91.3%), amoxicillin-clavulanic acid and amoxicillin (87%), penicillin-G and cloxacillin (82.6%), oxytetracycline (78.3%), and cefixime (73.9%). Screening of methicillin resistance gene revealed that 43.5% isolates of MRSA were positive for mecA gene. The high prevalence of MDR MRSA in frozen chicken meat samples in this study emphasizes the need for better sanitary education of food handlers in hygienic practices focusing on their potential role as reservoirs and spreaders of MRSA.

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“…Based on our results, there was no correlation between the MDR phenotype and the capacity of a given MRSA of producing a biofilm. With this regard, a recent study carried out by Ou et al [ 85 ] shows that there is a certain relationship between the level of antibiotic resistance and the biofilm forming ability in MRSA isolated from food from animals, but that the biofilm capacity of them is somehow impaired in those displaying the highest level of antibiotic resistance. Since MRSA biofilms can become recalcitrant contamination foci and a reservoir of several resistance genes, information about their adhesion capability is especially relevant to assess the potential hazard of a given subpopulation of MRSA, especially in those related with the food production chain.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain

Rodríguez-López

Filipello

Ciccio

et al. 2020

Foods

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The main aim of the present study was to evaluate the level of antibiotic resistance, prevalence and virulence features of methicillin-resistant Staphylococcus aureus (MRSA) isolated from heavy swine at abattoir level and farming environments in Lombardy (Northern Italy). With this scope, 88 different heavy swine farms were surveyed, obtaining a total of n = 440 animal swabs and n = 150 environmental swabs. A total of n = 87 MRSA isolates were obtained, with an overall MRSA incidence of 17.50% (n = 77) among animal samples and a 6.67% (n = 10) among environmental. Molecular characterisation using multilocus sequence typing (MLST) plus spa-typing showed that sequence type ST398/t899 and ST398/t011 were the most commonly isolated genotypes, although other relevant sequence types such as ST1 or ST97 were also found. A lack of susceptibility to penicillins, tetracycline and ceftiofur was detected in >91.95, 85.05 and 48.28% of the isolates, respectively. Resistance to doxycycline (32.18%), enrofloxacin (27.59%) and gentamicin (25.29%) was also observed. Additionally, a remarkable level of antibiotic multiresistance (AMR) was observed representing a 77.01% (n = 67) of the obtained isolates. Genetic analysis revealed that 97.70% and 77.01% of the isolates harboured at least one antibiotic resistance or enterotoxin gene, respectively, pointing out a high isolate virulence potential. Lastly, 55.17% (n = 48) were able to produce measurable amounts of biofilm after 24 h. In spite of the current programmes for antibiotic reduction in intensively farming, a still on-going high level of AMR and virulence potential in MRSA was demonstrated, making this pathogen a serious risk in swine production chain, highlighting once more the need to develop efficient, pathogen-specific control strategies.

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Prevalence of multidrug-resistant Staphylococcus aureus isolates with strong biofilm formation ability among animal-based food in Shanghai

Cited by 46 publications

References 22 publications

Occurrence and antimicrobial resistance of Salmonella isolated from retail meats in Anhui, China

Occurrence and antimicrobial resistance of Salmonella isolated from retail meats in Anhui, China

Prevalence and Multidrug Resistance Pattern of Methicillin Resistant S. aureus Isolated from Frozen Chicken Meat in Bangladesh

Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain

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